Reduce Electrical Overload Via Threaded Chinese Acupuncture in Nerve Electrical Therapy

Overview

Journal Bioact Mater

Date 2025 Jan 24

PMID 39850020

Authors

Yupu Liu

Yawei Du

Juan Wang

Longxi Wu

Feng Lin

Wenguo Cui

Affiliations

Soon will be listed here.

Abstract

Bioelectrical stimulation is a powerful technique used to promote tissue regeneration, but it can be hindered by an "electrical overload" phenomenon in the core region of stimulation. We develop a threaded microneedle electrode system that protects against "electrical overload" by delivering medicinal hydrogel microspheres into the core regions. The threaded needle body is coated with polydopamine and chitosan to enhance the adhesion of microspheres, which are loaded into the threaded grooves, allowing for their stereoscopic release in the core regions. After the electrode is inserted, the microspheres can be delivered three-dimensionally through physical swelling and the shear-thinning effect of chitosan, mitigating the electrical damage. Microspheres are designed to release alkylated vitamin B12 and vitamin E, providing antioxidant and cell protection effects upon activation, reducing reactive oxygen species (ROS) by 72.8 % and cell death by 59.5 %. In the model of peripheral nerve injury, the electrode system improves the overall antioxidant capacity by 78.5 % and protects the surrounding cells. Additionally, it leads to an improved nerve conduction velocity ratio of 41.9 % and sciatic nerve function index of 12.1 %, indicating enhanced neuroregeneration. The threaded microneedle electrode system offers a promising approach for nerve repair by inhibiting "electrical overload", potentially improving outcomes for tissue regeneration.

References

Kim S, Roy R, Zhong H, Suzuki H, Ambartsumyan L, Haddad F . Electromechanical stimulation ameliorates inactivity-induced adaptations in the medial gastrocnemius of adult rats. J Appl Physiol (1985). 2007; 103(1):195-205. DOI: 10.1152/japplphysiol.01427.2006. View

Lopes B, Sousa P, Alvites R, Branquinho M, Sousa A, Mendonca C . Peripheral Nerve Injury Treatments and Advances: One Health Perspective. Int J Mol Sci. 2022; 23(2). PMC: 8779751. DOI: 10.3390/ijms23020918. View

Hu C, Liu B, Huang X, Wang Z, Qin K, Sun L . Sea Cucumber-Inspired Microneedle Nerve Guidance Conduit for Synergistically Inhibiting Muscle Atrophy and Promoting Nerve Regeneration. ACS Nano. 2024; 18(22):14427-14440. DOI: 10.1021/acsnano.4c00794. View

Wu G, Wen X, Kuang R, Lui K, He B, Li G . Roles of Macrophages and Their Interactions with Schwann Cells After Peripheral Nerve Injury. Cell Mol Neurobiol. 2023; 44(1):11. PMC: 11407145. DOI: 10.1007/s10571-023-01442-5. View

Dong H, Wu H, Tang Y, Huang Y, Lin R, Zhao J . [AMPK regulates mitochondrial oxidative stress in C2C12 myotubes induced by electrical stimulations of different intensities]. Nan Fang Yi Ke Da Xue Xue Bao. 2018; 38(6):742-747. PMC: 6765703. View

Guan Y, Ren Z, Yang B, Xu W, Wu W, Li X . Dual-bionic regenerative microenvironment for peripheral nerve repair. Bioact Mater. 2023; 26:370-386. PMC: 10024190. DOI: 10.1016/j.bioactmat.2023.02.002. View

Tian M, Yang Y, Qin W, Liu B, Mou F, Zhu J . Electroacupuncture Promotes Nerve Regeneration and Functional Recovery Through Regulating lncRNA GAS5 Targeting miR-21 After Sciatic Nerve Injury. Mol Neurobiol. 2023; 61(2):935-949. PMC: 10861712. DOI: 10.1007/s12035-023-03613-3. View

Xu D, Zhang H, Wang Y, Zhang Y, Ye F, Lu L . Piezoelectric biomaterials for neural tissue engineering. Smart Med. 2024; 2(2):e20230002. PMC: 11235970. DOI: 10.1002/SMMD.20230002. View

Fan A . Anti-inflammatory mechanism of electroacupuncture involves the modulation of multiple systems, levels and targets and is not limited to "driving the vagus-adrenal axis". J Integr Med. 2023; 21(4):320-323. DOI: 10.1016/j.joim.2023.06.001. View

10.

Zhuang Y, Cui W . Biomaterial-based delivery of nucleic acids for tissue regeneration. Adv Drug Deliv Rev. 2021; 176:113885. DOI: 10.1016/j.addr.2021.113885. View

11.

Cheow W, Hadinoto K . Factors affecting drug encapsulation and stability of lipid-polymer hybrid nanoparticles. Colloids Surf B Biointerfaces. 2011; 85(2):214-20. DOI: 10.1016/j.colsurfb.2011.02.033. View

12.

Zhang R, Lao L, Ren K, Berman B . Mechanisms of acupuncture-electroacupuncture on persistent pain. Anesthesiology. 2013; 120(2):482-503. PMC: 3947586. DOI: 10.1097/ALN.0000000000000101. View

13.

Chen Y, Cai M, Shen B, Fan C, Zhou X . Electroacupuncture at Zusanli regulates the pathological phenotype of inflammatory bowel disease by modulating the NLRP3 inflammasome pathway. Immun Inflamm Dis. 2024; 12(8):e1366. PMC: 11310853. DOI: 10.1002/iid3.1366. View

14.

Lin F, Wang Z, Xiang L, Wu L, Liu Y, Xi X . Transporting Hydrogel via Chinese Acupuncture Needles for Lesion Positioning Therapy. Adv Sci (Weinh). 2022; 9(17):e2200079. PMC: 9189641. DOI: 10.1002/advs.202200079. View

15.

Li Y, Zheng J, Zhu Y, Qu Y, Suo R, Zhu Y . Neuroprotective effects of methylcobalamin in cerebral ischemia/reperfusion injury through activation of the ERK1/2 signaling pathway. Int Immunopharmacol. 2021; 99:108040. DOI: 10.1016/j.intimp.2021.108040. View

16.

Mahajan S, Fitzgerald M, Kenton K, Shott S, Brubaker L . Concentric needle electrodes are superior to perineal surface-patch electrodes for electromyographic documentation of urethral sphincter relaxation during voiding. BJU Int. 2005; 97(1):117-20. DOI: 10.1111/j.1464-410X.2006.05890.x. View

17.

Chen X, Ren L, Zhang H, Hu Y, Liao M, Shen Y . Basic fibroblast growth factor-loaded methacrylate gelatin hydrogel microspheres for spinal nerve regeneration. Smart Med. 2024; 2(2):e20220038. PMC: 11235853. DOI: 10.1002/SMMD.20220038. View

18.

Sun M, Wang X, Li Y, Yao W, Gu W . Mechanical effects of needle texture on acupoint tissue. J Integr Med. 2023; 21(3):254-267. DOI: 10.1016/j.joim.2023.03.005. View

19.

Oliveira J, Yanick C, Wein N, Gomez Limia C . Neuron-Schwann cell interactions in peripheral nervous system homeostasis, disease, and preclinical treatment. Front Cell Neurosci. 2023; 17:1248922. PMC: 10600466. DOI: 10.3389/fncel.2023.1248922. View

20.

Liu X, Zhuang Y, Huang W, Wu Z, Chen Y, Shan Q . Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy. Nat Commun. 2023; 14(1):4106. PMC: 10336067. DOI: 10.1038/s41467-023-39759-w. View