In Silico Prediction of Maize MicroRNA As a Xanthine Oxidase Inhibitor: A New Approach to Treating Hyperuricemia Patients

Overview

Journal Noncoding RNA

Date 2025 Jan 23

PMID 39846684

Authors

Manas Joshi

Mohd Mabood Khan

Affiliations

Soon will be listed here.

Abstract

Introduction: Hyperuricemia is characterized by increased uric acid (UA) in the body. The ability to block xanthine oxidase (XO) is a useful way to check how different bioactive molecules affect hyperuricemia. Previous reports showed the significant effect of corn against hyperuricemia disorder with its anti-XO activity. The identification of stable Zea mays miRNA (zma-miR) in humans has opened up a new avenue for speculation about its part in regulating novel human gene targets.

Aims: The aim of this study was to investigate the prospects of zma-miRs in XO gene regulation, the possible mechanism, and the interaction analysis of the zma-miR-XO mRNA transcript.

Method: Significant features of miRNA-mRNA interaction were revealed using two popular miRNA target prediction software-intaRNA (version 3.3.1) and RNA hybrid (version 2.2.1) Results: Only 12 zma-miR-156 variants, out of the 325 zma-miR's sequences reported in the miRNA database, efficiently interact with the 3'UTR of the XO gene. Characteristics of miRNA-mRNA interaction were as follows: the positioning of zma-miR-156 variants shows that they all have the same 11-mer binding sites, guanine (G), and uracil (U) loops at the 13th and 14th positions from the 5' end, and no G: U wobble pairing. These factors are related to the inhibition of functional mRNA expression. Additionally, the zma-miR-156 variants exhibit a single-base variation (SBV), which leads to distinct yet highly effective alterations in their interaction pattern with the XO mRNA transcript and the corresponding free energy values.

Conclusion: Therefore, we propose that zma-miR-156 variants may be a promising new bioactive compound against hyperuricemia and related diseases.

References

Teng Y, Ren Y, Sayed M, Hu X, Lei C, Kumar A . Plant-Derived Exosomal MicroRNAs Shape the Gut Microbiota. Cell Host Microbe. 2018; 24(5):637-652.e8. PMC: 6746408. DOI: 10.1016/j.chom.2018.10.001. View

Maziere P, Enright A . Prediction of microRNA targets. Drug Discov Today. 2007; 12(11-12):452-8. DOI: 10.1016/j.drudis.2007.04.002. View

Diez-Sainz E, Lorente-Cebrian S, Aranaz P, Amri E, Riezu-Boj J, Milagro F . miR482f and miR482c-5p from edible plant-derived foods inhibit the expression of pro-inflammatory genes in human THP-1 macrophages. Front Nutr. 2023; 10:1287312. PMC: 10719859. DOI: 10.3389/fnut.2023.1287312. View

Huddleston E, Gaffo A . Emerging strategies for treating gout. Curr Opin Pharmacol. 2022; 65:102241. DOI: 10.1016/j.coph.2022.102241. View

Kruger J, Rehmsmeier M . RNAhybrid: microRNA target prediction easy, fast and flexible. Nucleic Acids Res. 2006; 34(Web Server issue):W451-4. PMC: 1538877. DOI: 10.1093/nar/gkl243. View

Bardin T . Current management of gout in patients unresponsive or allergic to allopurinol. Joint Bone Spine. 2004; 71(6):481-5. DOI: 10.1016/j.jbspin.2004.07.006. View

Dong Q, Hu B, Zhang C . microRNAs and Their Roles in Plant Development. Front Plant Sci. 2022; 13:824240. PMC: 8895298. DOI: 10.3389/fpls.2022.824240. View

Nishino H, Murakoshi M, Tokuda H, Satomi Y . Cancer prevention by carotenoids. Arch Biochem Biophys. 2008; 483(2):165-8. DOI: 10.1016/j.abb.2008.09.011. View

Dubchak N, Falasca G . New and improved strategies for the treatment of gout. Int J Nephrol Renovasc Dis. 2011; 3:145-66. PMC: 3108771. DOI: 10.2147/IJNRD.S6048. View

10.

Saiyed A, Vasavada A, Johar S . Recent trends in miRNA therapeutics and the application of plant miRNA for prevention and treatment of human diseases. Futur J Pharm Sci. 2022; 8(1):24. PMC: 8972743. DOI: 10.1186/s43094-022-00413-9. View

11.

Gallo A, Tandon M, Alevizos I, Illei G . The majority of microRNAs detectable in serum and saliva is concentrated in exosomes. PLoS One. 2012; 7(3):e30679. PMC: 3302865. DOI: 10.1371/journal.pone.0030679. View

12.

Beta T, Hwang T . Influence of heat and moisture treatment on carotenoids, phenolic content, and antioxidant capacity of orange maize flour. Food Chem. 2018; 246:58-64. DOI: 10.1016/j.foodchem.2017.10.150. View

13.

Diez-Sainz E, Aranaz P, Amri E, Riezu-Boj J, Lorente-Cebrian S, Milagro F . Plant miR8126-3p and miR8126-5p Decrease Lipid Accumulation through Modulation of Metabolic Genes in a Human Hepatocyte Model That Mimics Steatosis. Int J Mol Sci. 2024; 25(3). PMC: 10855419. DOI: 10.3390/ijms25031721. View

14.

Hosoya T, Ogawa Y, Hashimoto H, Ohashi T, Sakamoto R . Comparison of topiroxostat and allopurinol in Japanese hyperuricemic patients with or without gout: a phase 3, multicentre, randomized, double-blind, double-dummy, active-controlled, parallel-group study. J Clin Pharm Ther. 2016; 41(3):290-7. DOI: 10.1111/jcpt.12391. View

15.

Gawlik-Dziki U, Dziki D, Swieca M, Nowak R . Mechanism of action and interactions between xanthine oxidase inhibitors derived from natural sources of chlorogenic and ferulic acids. Food Chem. 2017; 225:138-145. DOI: 10.1016/j.foodchem.2017.01.016. View

16.

Luo Y, Wang P, Wang X, Wang Y, Mu Z, Li Q . Detection of dietetically absorbed maize-derived microRNAs in pigs. Sci Rep. 2017; 7(1):645. PMC: 5428504. DOI: 10.1038/s41598-017-00488-y. View

17.

Georgakopoulos-Soares I, Parada G, Wong H, Medhi R, Furlan G, Munita R . Alternative splicing modulation by G-quadruplexes. Nat Commun. 2022; 13(1):2404. PMC: 9065059. DOI: 10.1038/s41467-022-30071-7. View

18.

Cavalieri D, Rizzetto L, Tocci N, Rivero D, Asquini E, Si-Ammour A . Plant microRNAs as novel immunomodulatory agents. Sci Rep. 2016; 6:25761. PMC: 4863160. DOI: 10.1038/srep25761. View

19.

Khan M, Sharma V, Serajuddin M, Kirabo A . Integrated grade-wise profiling analysis reveals potential plasma miR-373-3p as prognostic indicator in Prostate Cancer & its target KPNA2. Noncoding RNA Res. 2024; 9(3):954-963. PMC: 11063115. DOI: 10.1016/j.ncrna.2024.04.004. View

20.

Nile S, Park S . Antioxidant, α-glucosidase and xanthine oxidase inhibitory activity of bioactive compounds from maize (Zea mays L.). Chem Biol Drug Des. 2013; 83(1):119-25. DOI: 10.1111/cbdd.12205. View