Ozonized Water As a Promising Strategy to Remove Biofilm Formed by Spp. on Polyethylene and Polystyrene Surfaces

The dairy industry faces challenges in controlling spoilage microorganisms, particularly , known to form resilient biofilms. Conventional disinfection methods have limitations, prompting the exploration of eco-friendly alternatives like ozone. This study focused on biofilms on polystyrene and polyethylene surfaces, evaluating ozone efficacy when incorporated into different water sources and applied under static and dynamic conditions. Biofilm formation and removal were assessed with conventional microbiological and microscopic techniques. Despite variations in physicochemical properties, ozonized water from different sources showed similar effectiveness in removing biofilms. Dynamic ozone application was more efficient, achieving a 2.35 log CFU/coupon reduction on polyethylene surfaces, compared to a 1.05 log CFU/coupon reduction under static conditions. These findings highlight the potential of ozonized water for removing biofilms, especially under dynamic application. This eco-friendly approach could serve as an effective strategy to mitigate biofilm-related challenges in the dairy industry.