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Long Noncoding RNA DHRS4 Antisense RNA 1 Suppresses Osteosarcoma Cell Proliferation and Promotes Apoptosis Through a Competitive Endogenous RNA Mechanism

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Journal Sci Rep
Date 2025 Jan 22
PMID 39843945
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Abstract

Osteosarcoma (OS) is the most common primary malignant bone tumor. Recent evidence suggests that the novel long noncoding RNA DHRS4 antisense RNA 1 (DHRS4-AS1) serves an important role in cancer progression and metastasis. However, its function and molecular mechanism in OS remain largely unknown. In the present study, DHRS4-AS1 expression was detected in OS cells by quantitative PCR. Gain- and loss-of-function experiments were conducted to study the effects of DHRS4-AS1 on the proliferation and apoptosis of OS cells. The potential mechanism of DHRS4-AS1 was examined through bioinformatics analysis and rescue experiments. DHRS4-AS1 was downregulated in OS cell lines. DHRS4-AS1 depletion promoted proliferation and inhibited apoptosis in OS cells, whereas DHRS4-AS1 overexpression had the opposite effects. Further research suggested that DHRS4-AS1 inhibited OS progression by regulating the microRNA-362-5p/aminopeptidase puromycin sensitive axis. The present findings suggested that DHRS4-AS1 may serve as a potential therapeutic target for OS.

References
1.
Yang P, Ni F, Deng R, Qiang G, Zhao H, Yang M . MiR-362-5p promotes the malignancy of chronic myelocytic leukaemia via down-regulation of GADD45α. Mol Cancer. 2015; 14:190. PMC: 4636774. DOI: 10.1186/s12943-015-0465-3. View

2.
Wu K, Yang L, Chen J, Zhao H, Wang J, Xu S . miR-362-5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3-kinase-C2β. FEBS Lett. 2015; 589(15):1911-9. DOI: 10.1016/j.febslet.2015.05.056. View

3.
Park J, Cheung N . Promise and Challenges of T Cell Immunotherapy for Osteosarcoma. Int J Mol Sci. 2023; 24(15). PMC: 10419531. DOI: 10.3390/ijms241512520. View

4.
Luan F, Chen W, Chen M, Yan J, Chen H, Yu H . An autophagy-related long non-coding RNA signature for glioma. FEBS Open Bio. 2019; 9(4):653-667. PMC: 6443865. DOI: 10.1002/2211-5463.12601. View

5.
Sadykova L, Ntekim A, Muyangwa-Semenova M, Rutland C, Jeyapalan J, Blatt N . Epidemiology and Risk Factors of Osteosarcoma. Cancer Invest. 2020; 38(5):259-269. DOI: 10.1080/07357907.2020.1768401. View