Formation and Stability of Green and Low-cost Magnetoliposomes of the Soy Lecithin, Stigmasterol, and β-sitosterol for Hyperthermia Treatments

Overview

Journal Sci Rep

Date 2025 Jan 22

PMID 39843908

Authors

Rosangela Maria Ferreira da Costa E Silva

Angela Leao Andrade

Erico Tadeu Fraga Freitas

Thalita Marcolan Valverde

Luciano Roni Silva Lara

Darliane Aparecida Martins

Jorge Luis Lopez

Humberto Osorio Stumpf

Clesia Cristina Nascentes

Alfredo Miranda de Goes

Rosana Zacarias Domingues

Affiliations

Soon will be listed here.

Abstract

Magnetoliposomes containing magnetite, soy lecithin, stigmasterol, and beta-sitosterol of the mean size minor than 160 nm were obtained by a scalable and green process using autoclave and sonication without organic solvents. The formation, size of the liposome, linkage, and encapsulation of the magnetite were evaluated by Cryo-TEM. The stability of magnetoliposomes after storage for 6 months at 4 °C was improved by liposome size, the ability of soy lecithin to preserve the magnetite phase against oxidation, pH, polydispersity index, and zeta potential. The iron oxide phase stability was assessed using no conventional X-ray diffraction (high-resolution transmission electron microscopy), energy loss electron spectroscopy, and selected area electron diffraction) in time zero (fresh sample) and 6 months. The high zeta potential measured for magnetoliposomes, │53│ mV, indicated a low tendency to agglomerate. Lip-FeO@lecithin with concentrations of 0.58 mg mL of liposome showed high cell viability and are potential candidates for drug delivery and hyperthermia treatments in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays.

References

Chen L, Chen Y, Su C, Wong W, Sheu M, Ho H . Development and Characterization of Lecithin-based Self-assembling Mixed Polymeric Micellar (saMPMs) Drug Delivery Systems for Curcumin. Sci Rep. 2016; 6():37122. PMC: 5111064. DOI: 10.1038/srep37122. View

Kayode Sodipo B, Abdul Aziz A . Non-seeded synthesis and characterization of superparamagnetic iron oxide nanoparticles incorporated into silica nanoparticles via ultrasound. Ultrason Sonochem. 2014; 23:354-9. DOI: 10.1016/j.ultsonch.2014.09.011. View

Golla-Schindler U, Hinrichs R, Bomati-Miguel O, Putnis A . Determination of the oxidation state for iron oxide minerals by energy-filtering TEM. Micron. 2006; 37(5):473-7. DOI: 10.1016/j.micron.2005.11.002. View

Phan T, Moorthy M, Kang H, Nam S, Lee Y, Oh J . Coating Chitosan Thin Shells: A Facile Technique to Improve Dispersion Stability of Magnetoliposomes. J Nanosci Nanotechnol. 2018; 18(1):583-590. DOI: 10.1166/jnn.2018.13934. View

Meure L, Foster N, Dehghani F . Conventional and dense gas techniques for the production of liposomes: a review. AAPS PharmSciTech. 2008; 9(3):798-809. PMC: 2977034. DOI: 10.1208/s12249-008-9097-x. View

Rocas P, Fernandez Y, Garcia-Aranda N, Foradada L, Calvo P, Aviles P . Improved pharmacokinetic profile of lipophilic anti-cancer drugs using ανβ3-targeted polyurethane-polyurea nanoparticles. Nanomedicine. 2017; 14(2):257-267. DOI: 10.1016/j.nano.2017.10.009. View

Chen Y, Van Minh L, Liu J, Angelov B, Drechsler M, Garamus V . Baicalin loaded in folate-PEG modified liposomes for enhanced stability and tumor targeting. Colloids Surf B Biointerfaces. 2016; 140:74-82. DOI: 10.1016/j.colsurfb.2015.11.018. View

Tan Y, Qi J, Lu Y, Hu F, Yin Z, Wu W . Lecithin in mixed micelles attenuates the cytotoxicity of bile salts in Caco-2 cells. Toxicol In Vitro. 2012; 27(2):714-20. DOI: 10.1016/j.tiv.2012.11.018. View

Mitchell D . DiffTools: electron diffraction software tools for DigitalMicrograph. Microsc Res Tech. 2008; 71(8):588-93. DOI: 10.1002/jemt.20591. View

10.

Harashima H, Sakata K, Funato K, Kiwada H . Enhanced hepatic uptake of liposomes through complement activation depending on the size of liposomes. Pharm Res. 1994; 11(3):402-6. DOI: 10.1023/a:1018965121222. View

11.

Clares B, Gallardo V, Medina M, Ruiz M . Multilamellar liposomes of triamcinolone acetonide: preparation, stability, and characterization. J Liposome Res. 2009; 19(3):197-206. DOI: 10.1080/08982100902736571. View

12.

Al-Jamal W, Al-Jamal K, Bomans P, Frederik P, Kostarelos K . Functionalized-quantum-dot-liposome hybrids as multimodal nanoparticles for cancer. Small. 2008; 4(9):1406-15. DOI: 10.1002/smll.200701043. View

13.

Perez S, Gandola Y, Carlucci A, Gonzalez L, Turyn D, Bregni C . Formulation Strategies, Characterization, and In Vitro Evaluation of Lecithin-Based Nanoparticles for siRNA Delivery. J Drug Deliv. 2012; 2012:986265. PMC: 3335242. DOI: 10.1155/2012/986265. View

14.

Andrade A, Fabris J, Domingues R, Pereira M . Current Status of Magnetite-Based Core@Shell Structures for Diagnosis and Therapy in Oncology Short running title: Biomedical Applications of Magnetite@Shell Structures. Curr Pharm Des. 2015; 21(37):5417-33. DOI: 10.2174/1381612821666150917093543. View

15.

Danaei M, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A . Impact of Particle Size and Polydispersity Index on the Clinical Applications of Lipidic Nanocarrier Systems. Pharmaceutics. 2018; 10(2). PMC: 6027495. DOI: 10.3390/pharmaceutics10020057. View

16.

Woodle M, Engbers C, Zalipsky S . New amphipatic polymer-lipid conjugates forming long-circulating reticuloendothelial system-evading liposomes. Bioconjug Chem. 1994; 5(6):493-6. DOI: 10.1021/bc00030a001. View

17.

Ali F, Reinert L, Leveque J, Duclaux L, Muller F, Saeed S . Effect of sonication conditions: solvent, time, temperature and reactor type on the preparation of micron sized vermiculite particles. Ultrason Sonochem. 2013; 21(3):1002-9. DOI: 10.1016/j.ultsonch.2013.10.010. View

18.

Allen T, Chonn A . Large unilamellar liposomes with low uptake into the reticuloendothelial system. FEBS Lett. 1987; 223(1):42-6. DOI: 10.1016/0014-5793(87)80506-9. View

19.

Fenske D, Cullis P . Entrapment of small molecules and nucleic acid-based drugs in liposomes. Methods Enzymol. 2005; 391:7-40. DOI: 10.1016/S0076-6879(05)91001-X. View

20.

Walde P, Ichikawa S . Enzymes inside lipid vesicles: preparation, reactivity and applications. Biomol Eng. 2001; 18(4):143-77. DOI: 10.1016/s1389-0344(01)00088-0. View