Occurrence of Antibiotic-Resistant Bacteria in Urban Surface Water Sources in Bangladesh

Overview

Journal Curr Microbiol

Date 2025 Jan 20

PMID 39833477

Authors

M M Majedul Islam

Md Atikul Islam

Farzana Ashrafi Neela

Abul Farah Md Hasanuzzaman

Affiliations

Soon will be listed here.

Abstract

Infections caused by antibiotic-resistant bacteria (ARB) result in an estimated 1.27 million human deaths annually worldwide. Surface waters are impacted by anthropogenic factors, which contribute to the emergence and spread of ARB in the aquatic environment. While research on antibiotic resistance in surface waters has increased recently in developing nations, including Bangladesh, especially in aquaculture, such studies are still limited in Bangladesh compared to developed nations. In this study, bacteria strains isolated from three rivers and two lakes in Khulna city, Bangladesh were characterized for their antibiotic resistance using disk diffusion method. Of the 106 bacterial isolates from 180 surface water samples, the majority exhibited resistance to Ciprofloxacin (75.0-87.5%) and Ceftriaxone (65.6-78.1%), while resistance to Ampicillin was comparatively lower (9.4-18.8%). Notably, the prevalence of ARB was observed to be higher during the wet seasons compared to the dry seasons. The 16S rRNA gene analysis showed that Shigella flexneri was the most dominant (17.9%) bacterium among the ARB cultured from surface waters, followed by Escherichia fergusonii (12.5%), Proteus mirabilis (10.7%), and Enterobacter quasiroggenkampii (8.9%). At the genus level, Enterobacter (23.5%), Shigella (20.6%), and Escherichia spp. (14.7%) were the most abundant among the ARB in both river and lake samples. The findings of this study highlight the prevalence of antimicrobial resistance in surface water sources and suggest the need for enhanced monitoring and improved disposal practices to mitigate potential public health risks.

References

Yuan Q, Guo M, Yang J . Fate of antibiotic resistant bacteria and genes during wastewater chlorination: implication for antibiotic resistance control. PLoS One. 2015; 10(3):e0119403. PMC: 4349789. DOI: 10.1371/journal.pone.0119403. View

Bockelmann U, Dorries H, Ayuso-Gabella M, Salgot de Marcay M, Tandoi V, Levantesi C . Quantitative PCR monitoring of antibiotic resistance genes and bacterial pathogens in three European artificial groundwater recharge systems. Appl Environ Microbiol. 2008; 75(1):154-63. PMC: 2612202. DOI: 10.1128/AEM.01649-08. View

Ben Y, Fu C, Hu M, Liu L, Wong M, Zheng C . Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review. Environ Res. 2018; 169:483-493. DOI: 10.1016/j.envres.2018.11.040. View

Lynch 3rd J, Clark N, Zhanel G . Evolution of antimicrobial resistance among Enterobacteriaceae (focus on extended spectrum β-lactamases and carbapenemases). Expert Opin Pharmacother. 2013; 14(2):199-210. DOI: 10.1517/14656566.2013.763030. View

Ahmed I, Rabbi M, Sultana S . Antibiotic resistance in Bangladesh: A systematic review. Int J Infect Dis. 2019; 80:54-61. DOI: 10.1016/j.ijid.2018.12.017. View

Williams A, Rousham E, Neal A, Amin M, Hobman J, Stekel D . Impact of contrasting poultry exposures on human, poultry, and wastewater antibiotic resistomes in Bangladesh. Microbiol Spectr. 2023; 11(6):e0176323. PMC: 10714819. DOI: 10.1128/spectrum.01763-23. View

Ahmed H, Zolfo M, Williams A, Ashubwe-Jalemba J, Tweya H, Adeapena W . Antibiotic-Resistant Bacteria in Drinking Water from the Greater Accra Region, Ghana: A Cross-Sectional Study, December 2021-March 2022. Int J Environ Res Public Health. 2022; 19(19). PMC: 9566567. DOI: 10.3390/ijerph191912300. View

Thornber K, Bashar A, Ahmed M, Bell A, Trew J, Hasan M . Antimicrobial Resistance in Aquaculture Environments: Unravelling the Complexity and Connectivity of the Underlying Societal Drivers. Environ Sci Technol. 2022; 56(21):14891-14903. PMC: 9631993. DOI: 10.1021/acs.est.2c00799. View

Asaduzzaman M, Rousham E, Unicomb L, Islam M, Amin M, Rahman M . Spatiotemporal distribution of antimicrobial resistant organisms in different water environments in urban and rural settings of Bangladesh. Sci Total Environ. 2022; 831:154890. DOI: 10.1016/j.scitotenv.2022.154890. View

10.

Reynolds K, Watt P, Boone S, Gerba C . Occurrence of bacteria and biochemical markers on public surfaces. Int J Environ Health Res. 2005; 15(3):225-34. DOI: 10.1080/09603120500115298. View

11.

Chowdhury S, Rheman S, Debnath N, Delamare-Deboutteville J, Akhtar Z, Ghosh S . Antibiotics usage practices in aquaculture in Bangladesh and their associated factors. One Health. 2022; 15:100445. PMC: 9582543. DOI: 10.1016/j.onehlt.2022.100445. View

12.

Adomako L, Yirenya-Tawiah D, Nukpezah D, Abrahamya A, Labi A, Grigoryan R . Reduced Bacterial Counts from a Sewage Treatment Plant but Increased Counts and Antibiotic Resistance in the Recipient Stream in Accra, Ghana-A Cross-Sectional Study. Trop Med Infect Dis. 2021; 6(2). PMC: 8163181. DOI: 10.3390/tropicalmed6020079. View

13.

Rahman M, Nonaka L, Tago R, Suzuki S . Occurrence of two genotypes of tetracycline (TC) resistance gene tet(M) in the TC-resistant bacteria in marine sediments of Japan. Environ Sci Technol. 2008; 42(14):5055-61. DOI: 10.1021/es702986y. View

14.

Frank J, Reich C, Sharma S, Weisbaum J, Wilson B, Olsen G . Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol. 2008; 74(8):2461-70. PMC: 2293150. DOI: 10.1128/AEM.02272-07. View

15.

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

16.

Navarro A, Sanseverino I, Cappelli F, Lahm A, Niegowska M, Fabbri M . Study of antibiotic resistance in freshwater ecosystems with low anthropogenic impact. Sci Total Environ. 2022; 857(Pt 3):159378. DOI: 10.1016/j.scitotenv.2022.159378. View

17.

Neyestani Z, Khademi F, Teimourpour R, Amani M, Arzanlou M . Prevalence and mechanisms of ciprofloxacin resistance in Escherichia coli isolated from hospitalized patients, healthy carriers, and wastewaters in Iran. BMC Microbiol. 2023; 23(1):191. PMC: 10351176. DOI: 10.1186/s12866-023-02940-8. View

18.

Di Cesare A, Eckert E, Rogora M, Corno G . Rainfall increases the abundance of antibiotic resistance genes within a riverine microbial community. Environ Pollut. 2017; 226:473-478. DOI: 10.1016/j.envpol.2017.04.036. View

19.

Crettels L, Champon L, Burlion N, Delree E, Saegerman C, Thiry D . Antimicrobial resistant prevalence in freshwaters in Belgium and human exposure risk assessment. Heliyon. 2023; 9(6):e16538. PMC: 10241855. DOI: 10.1016/j.heliyon.2023.e16538. View

20.

Elbehiry A, Al Shoaibi M, Alzahrani H, Ibrahem M, Moussa I, Alzaben F . Enterobacter cloacae from urinary tract infections: frequency, protein analysis, and antimicrobial resistance. AMB Express. 2024; 14(1):17. PMC: 10853136. DOI: 10.1186/s13568-024-01675-7. View