In Vitro Antitumor Effects of Methanolic Extracts of Three Ganoderema Mushrooms

Overview

Journal Sci Rep

Date 2025 Jan 17

PMID 39824924

Authors

Elshahat A Toson

Amira A El-Fallal

Marwa A Oransa

Hoda M El-Gharabawy

Affiliations

Soon will be listed here.

Abstract

Ganoderma mushrooms have a variety of pharmacological activities and may have antitumor effects. Therefore, the antitumor activity of the methanolic fruiting body extracts of three Ganoderma spp. will be evaluated by estimating cell viability, cell cycle parameters and the mode of cellular death. In this regard, Sulfo-rhodamine B staining and flow cytometry were used. Hepatocellular carcinoma (HepG2) and breast ductal carcinoma (T-47D) cell lines were used as cancer models, while mouse normal liver (BNL) and oral epithelial cell (OEC) lines were used as respective controls. The results revealed that Ganoderma resinaceum extract decreased the viability of BNL at an IC > 100 µg/mL but not that of HepG2 at an IC of 72.32 µg/mL. Additionally, Ganoderma australe and Ganoderma mbrekobenum decreased the viability of OEC cell line at an IC of 328.29 and 271.56 µg/ mL, respectively. On the other hand, the IC of T-47D were 221.95 and 236.45 µg/mL, respectively. The three extracts arrested the cell life cycle at the G1 phase in each case. G. resinaceum extract stimulated total apoptosis (Q2 + Q4) of 19.99% with low necrosis (Q1). However, the percentages of total cell necrosis in the T-47D cell line treated with the other two extracts were 31.10% and 18.28%, respectively while the percentages of total cell apoptosis were 6.83% and 1.78%, respectively. Thus, G. resinaceum significantly inhibited the viability of the HepG2 cell line, while both the G. australe and G. mbrekobenum extracts significantly decreased the viability of the T-47D cell line. These results may encourage speculation about their possible use for the therapeutic management of hepatocellular carcinoma and breast ductal carcinoma after further investigation.

References

Hanyu X, Lanyue L, Miao D, Wentao F, Cangran C, Hui S . Effect of Ganoderma applanatum polysaccharides on MAPK/ERK pathway affecting autophagy in breast cancer MCF-7 cells. Int J Biol Macromol. 2020; 146:353-362. DOI: 10.1016/j.ijbiomac.2020.01.010. View

Jiang J, Slivova V, Harvey K, Valachovicova T, Sliva D . Ganoderma lucidum suppresses growth of breast cancer cells through the inhibition of Akt/NF-kappaB signaling. Nutr Cancer. 2004; 49(2):209-16. DOI: 10.1207/s15327914nc4902_13. View

Elmore S . Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007; 35(4):495-516. PMC: 2117903. DOI: 10.1080/01926230701320337. View

Jemal A, Bray F, Center M, Ferlay J, Ward E, Forman D . Global cancer statistics. CA Cancer J Clin. 2011; 61(2):69-90. DOI: 10.3322/caac.20107. View

DeSantis C, Fedewa S, Sauer A, Kramer J, Smith R, Jemal A . Breast cancer statistics, 2015: Convergence of incidence rates between black and white women. CA Cancer J Clin. 2015; 66(1):31-42. DOI: 10.3322/caac.21320. View

McGuire S . World Cancer Report 2014. Geneva, Switzerland: World Health Organization, International Agency for Research on Cancer, WHO Press, 2015. Adv Nutr. 2016; 7(2):418-9. PMC: 4785485. DOI: 10.3945/an.116.012211. View

El-Sherif N, Ahmed S, Ibrahim A, Habib E, El-Fallal A, El-Sayed A . Ergosterol Peroxide from the Egyptian Red Lingzhi or Reishi Mushroom, Ganoderma resinaceum (Agaricomycetes), Showed Preferred Inhibition of MCF-7 over MDA-MB-231 Breast Cancer Cell Lines. Int J Med Mushrooms. 2020; 22(4):389-396. DOI: 10.1615/IntJMedMushrooms.2020034223. View

Wu X, Jiang L, Zhang Z, He Y, Teng Y, Li J . Pancreatic cancer cell apoptosis is induced by a proteoglycan extracted from . Oncol Lett. 2020; 21(1):34. PMC: 7693130. DOI: 10.3892/ol.2020.12295. View

Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D . New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990; 82(13):1107-12. DOI: 10.1093/jnci/82.13.1107. View

10.

Fekry M, Ezzat S, Salama M, Alshehri O, Al-Abd A . Bioactive glycoalkaloides isolated from Solanum melongena fruit peels with potential anticancer properties against hepatocellular carcinoma cells. Sci Rep. 2019; 9(1):1746. PMC: 6370831. DOI: 10.1038/s41598-018-36089-6. View

11.

Sedky N, El Gammal Z, Wahba A, Mosad E, Waly Z, El-Fallal A . The molecular basis of cytotoxicity of α-spinasterol from Ganoderma resinaceum: Induction of apoptosis and overexpression of p53 in breast and ovarian cancer cell lines. J Cell Biochem. 2017; 119(5):3892-3902. DOI: 10.1002/jcb.26515. View

12.

Smina T, Nitha B, Devasagayam T, Janardhanan K . Ganoderma lucidum total triterpenes induce apoptosis in MCF-7 cells and attenuate DMBA induced mammary and skin carcinomas in experimental animals. Mutat Res Genet Toxicol Environ Mutagen. 2016; 813:45-51. DOI: 10.1016/j.mrgentox.2016.11.010. View

13.

Bhardwaj M, Leli N, Koumenis C, Amaravadi R . Regulation of autophagy by canonical and non-canonical ER stress responses. Semin Cancer Biol. 2019; 66:116-128. PMC: 7325862. DOI: 10.1016/j.semcancer.2019.11.007. View

14.

Liu H, Qiao J, Shangguan J, Zhu J . Effects of glutamate oxaloacetate transaminase on reactive oxygen species in Ganoderma lucidum. Appl Microbiol Biotechnol. 2023; 107(5-6):1845-1861. DOI: 10.1007/s00253-023-12417-3. View

15.

Chen C, Li P, Li Y, Yao G, Xu J . Antitumor effects and mechanisms of extracts and spores oil. Oncol Lett. 2016; 12(5):3571-3578. PMC: 5104053. DOI: 10.3892/ol.2016.5059. View

16.

Ren F, Cao K, Gong R, Yu M, Tao P, Xiao Y . The role of post-transcriptional modification on a new tRNA identified from Ganoderma lucidum in its fragments' cytotoxicity on cancer cells. Int J Biol Macromol. 2023; 229:885-895. DOI: 10.1016/j.ijbiomac.2022.12.327. View

17.

Cristiano M, Mancuso A, Fresta M, Torella D, De Gaetano F, Ventura C . Topical Unsaturated Fatty Acid Vesicles Improve Antioxidant Activity of Ammonium Glycyrrhizinate. Pharmaceutics. 2021; 13(4). PMC: 8070842. DOI: 10.3390/pharmaceutics13040548. View

18.

Alaufi O, Noorwali A, Zahran F, Al-Abd A, Al-Attas S . Cytotoxicity of thymoquinone alone or in combination with cisplatin (CDDP) against oral squamous cell carcinoma in vitro. Sci Rep. 2017; 7(1):13131. PMC: 5640598. DOI: 10.1038/s41598-017-13357-5. View

19.

Hsu S, Ou C, Li J, Chuang T, Kuo H, Liu J . Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G(2)/M cell cycle arrest. J Ethnopharmacol. 2008; 120(3):394-401. DOI: 10.1016/j.jep.2008.09.025. View

20.

Goel P, Alam O, Naim M, Nawaz F, Iqbal M, Alam M . Recent advancement of piperidine moiety in treatment of cancer- A review. Eur J Med Chem. 2018; 157:480-502. DOI: 10.1016/j.ejmech.2018.08.017. View