Testing the Kinetic Tradeoff Between Bicarbonate Versus Phosphoenolpyruvate Affinity and Glucose-6 Phosphate Response of Phosphoenolpyruvate Carboxylase from Two C grasses

Overview

Journal Photosynth Res

Publisher Springer

Date 2025 Jan 15

PMID 39812731

Authors

Ryan L Wessendorf

Matt Stata

Asaph B Cousins

Affiliations

Soon will be listed here.

Abstract

Phosphoenolpyruvate (PEP) carboxylase (PEPC) has an anaplerotic role in central plant metabolism but also initiates the carbon concentrating mechanism during C photosynthesis. The C PEPC has different binding affinities (K) for PEP (K) and HCO (K), and allosteric regulation by glucose-6-phosphate (G6-P) compared to non-photosynthetic isoforms. These differences are linked to specific changes in amino acids within PEPC. For example, region II (residues 302-433, Zea mays numbering) has been identified as important for G6-P regulation and within this region residue 353 may be conserved in C PEPC enzymes. Additionally, residue 780 influences the C PEPC kinetic properties and may interact with region II as well as residue 353 to influence G6-P regulation. We test the hypothesis that variation within region II, including residue 353, and their interactions with residue 780 influence the kinetic and allosteric regulation by G6-P of two C PEPC isozymes from two C grasses. The data does not support a kinetic tradeoff between K and K in these PEPC isozymes. Additionally, these enzymes had different response to G6-P that was only partially attributed to region II, residue 353 and residue 780. This data provides new insights into factors influencing the kinetic variation of C PEPC isozymes.

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