Kinetics of Reformation of the S State Capable of Progressing to the S State After the O Release by Photosystem II

Overview

Journal Photosynth Res

Publisher Springer

Date 2025 Jan 14

PMID 39810006

Authors

Alain Boussac

Julien Selles

Miwa Sugiura

Affiliations

Soon will be listed here.

Abstract

The active site for water oxidation in photosystem II (PSII) comprises a MnCaO cluster adjacent to a redox-active tyrosine residue (Tyr). During the water-splitting process, the enzyme transitions through five sequential oxidation states (S to S), with O evolution occurring during the STyr· to STyr transition. Chloride also plays a role in this mechanism. Using PSII from Thermosynechococcus vestitus, where Ca and Cl were replaced with Sr and Br to slow the STyr· to STyr + O transition (t ~ 5 ms at room temperature), it was observed that the recovery of a S state, defined as the state able to progress to S, exhibits similar kinetics (t ~ 5 ms). This suggests that in CaCl-PSII, the reformation of the functional S state directly follows the STyr· to STyr + O transition, with no additional delay required for the insertion of a new substrate water molecule (O5) and associated protons.

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