Pathway of Typical β-Lactam Antibiotics Degradation by Black Soldier Fly and Response Characteristic of Its Intestinal Microbes

To effectively address the contamination caused by antibiotic misuse, this study was conducted to enhance the removal of amoxicillin (AMX) and penicillin sodium (PEN) by incorporating black soldier fly larvae (BSFL). The results showed that BSFL increased the degradation rates of AMX and PEN to 71.00 % and 80.89 %, respectively, and shortened their half-lives to 238 h and 160 h. Proteobacteria (26.2 %-82.0 %), Firmicutes (13.3 %-54.0 %), Acinobacteriota (2.1 %-23.4 %), and Bacteroidota (1.3 %-10.1 %) were the intestinal dominant microorganisms during transformation. Five bacteria with β-lactam antibiotic resistance in the BSFL gut were isolated, among which Morganella morganii demonstrated strong antibiotic tolerance and high removal rates of AMX and PEN in both in vitro and in vivo experiments, ranging from 58.99 % to 95.87 %. BSFL intestinal bacteria disrupted the quaternary pharmacophore of AMX and PEN, breaking them down into at least seven and five metabolites, respectively.