Tay-Sachs and Sandhoff Diseases: Diffusion Tensor Imaging and Correlational Fiber Tractography Findings Differentiate Late-onset GM2 Gangliosidosis

Overview

Journal medRxiv

Date 2025 Jan 13

PMID 39802759

Authors

Connor J Lewis

Selby I Chipman

Jean M Johnston

Maria T Acosta

Camilo Toro

Cynthia J Tifft

Affiliations

Soon will be listed here.

Abstract

GM2 gangliosidosis is lysosomal storage disorder caused by deficiency of the heterodimeric enzyme β-hexosaminidase A. Tay-Sachs disease is caused by variants in encoding the α-subunit and Sandhoff disease is caused by variants in encoding the β-subunit. Due to shared clinical and biochemical findings, the two have been considered indistinguishable. We applied diffusion tensor imaging (DTI) and correlational fiber tractography to assess phenotypic differences in these two diseases. 40 DTI scans from 16 Late-Onset GM2 patients (NCT00029965) with either Sandhoff (n = 4), or Tay-Sachs (n = 12) disease. DTI metrics including fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), axial diffusivity (AD), and quantitative anisotropy (QA) were calculated in fiber tracts throughout the whole brain, arcuate fasciculus, corpus callosum, and cerebellum. Correlational tractography was also performed to identify fiber tracts with group wide differences in DTI metrics between Tay-Sachs and Sandhoff patients. A linear mixed effects model was used to analyze the differences between Tay-Sachs and Sandhoff patients. Tay-Sachs patients had higher MD in the left cerebellum ( = 0.003703), right cerebellum ( = 0.003435), superior cerebellar peduncle (SCP, = 0.007332), and vermis ( = 0.01007). Sandhoff patients had higher FA in the left cerebellum ( = 0.005537), right cerebellum ( = 0.01905), SCP ( = 0.02844), and vermis ( = 0.02469). Correlational fiber tractography identified fiber tracts almost exclusively in cerebellar pathways with higher FA and QA, and lower MD, AD, and RD in Sandhoff patients compared to Tay-Sachs patients. Our study shows neurobiological differences between these two related disorders. To our knowledge, this is the first study using correlational tractography in a lysosomal storage disorder demonstrating these differences. This result indicates a greater burden of cerebellar pathology in Tay-Sachs patients compared with Sandoff patients.

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