The Phytochemical Composition and Antioxidant Activity of Blossoms and Rhizome Ethanol Extracts

Overview

Journal Nutrients

Date 2025 Jan 11

PMID 39796439

Authors

Anca Elena But

Raluca Maria Pop

Georg Friedrich Binsfeld

Floricuta Ranga

Meda Sandra Orasan

Andra Diana Cecan

Iulia Ioana Morar

Elisabeta Ioana Chera

Teodora Irina Bonci

Lia Oxana Usatiuc

Madalina Ticolea

Florinela Adriana Catoi

Alina Elena Parvu

Mircea Constantin Dinu Ghergie

Affiliations

Soon will be listed here.

Abstract

Background: Inflammation-induced oxidative stress is a pathophysiological mechanism of inflammatory diseases. Treatments targeting oxidative stress can reduce inflammatory tissue damage.

Objectives: This study aimed to conduct phytochemical analysis and evaluate the antioxidant effects of the hydroalcoholic extract of blossoms () and rhizomes ().

Materials And Methods: The phytochemical analysis was carried out by measuring the total polyphenol content, total flavonoid content, and polyphenolic compounds' HPLC-ESI MS. The antioxidant activity was evaluated in vitro through HO DPPH, FRAP, and NO scavenging assays. An in vivo experiment was performed on rats with turpentine oil-induced acute inflammation. Treatments were administrated orally for 10 days, with three dilutions of each extract (100%, 50%, 25%), and compared to the CONTROL, inflammation, Diclofenac, and Trolox groups. In vivo, the antioxidant activity was evaluated by measuring the total antioxidant capacity (TAC), total oxidative status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein products (AOPP), and total thiols (SH).

Results: The phytochemical analysis found a high content of phenolic compounds in both extracts, and the in vitro antioxidant activity was significant. In vivo, and extracts proved to be effective in increasing TAC and lowering oxidative stress markers, respectively, the TOS, OSI, MDA, and NO levels. The effects were dose-dependent, with the lower concentrations being more efficient antioxidants. and extract effects were as good as those of trolox and diclofenac.

Conclusions: Treatment with and alleviated inflammation-induced oxidative stress. These findings suggest that and extracts could be a promising adjuvant antioxidant therapy in inflammatory diseases.

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