The Use of a Novel Container for Secured Transport and Storage of Biological Material for Quantitative Human RNA Analysis

Overview

Journal Int J Mol Sci

Publisher MDPI

Date 2025 Jan 11

PMID 39796092

Authors

Dorota Kostrzewa-Nowak

Alicja Trzeciak-Ryczek

Klaudyna Lewandowska

Thierry van de Wetering

Andrzej Ciechanowicz

Robert Nowak

Affiliations

Soon will be listed here.

Abstract

The transport of biological materials must protect samples from degradation and ensure courier safety. The main goal of this study was to evaluate the usefulness of a new type of container designed for the secured transport of biological material for storing samples for quantitative RNA analyses. This was achieved by analyzing changes in the expression of selected human leucocyte housekeeping genes (, , and ) using reverse transcription quantitative PCR (RT-qPCR) and digital PCR (RT-dPCR) techniques. Digital PCR analysis evidenced that the novel type of container retains a higher count of analyzed gene copies per µL of samples during 5 h of incubation time. The container ensures a low maintenance temperature for several hours, making it useful for sustaining the conditions for transporting biological samples. This novel container can be used to store and transport biological material to be analyzed by molecular techniques and can retain the stability of total RNA over several hours.

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