γ-Aminobutyric Acid Alleviates Programmed Cell Death in Two Species Under Cadmium Stress

Overview

Journal Int J Mol Sci

Publisher MDPI

Date 2025 Jan 11

PMID 39795987

Authors

Zhong-Wei Zhang

Tao-Tao Dang

Xin-Yue Yang

Lin-Bei Xie

Yang-Er Chen

Ming Yuan

Guang-Deng Chen

Jian Zeng

Shu Yuan

Affiliations

Soon will be listed here.

Abstract

Previous studies have demonstrated that γ-Aminobutyric acid (GABA) effectively alleviates heavy metal stresses by maintaining the redox balance and reducing the accumulation of reactive oxygen species (ROS). However, little is known about the role of GABA on programmed cell death (PCD) under Cd treatments in plants. The present study investigated the effects of GABA on Cd-induced PCD in two species, oilseed rape (, ), and black mustard (, ). We observed that GABA significantly alleviated Cd-induced PCD by enhancing antioxidant systems, inhibiting chromatin condensation in the nucleus, and reducing DNA fragmentation under Cd stress. Moreover, GABA may not only reduce caspase-3-like activity by repressing gene expression, but also regulate transcription of PCD-related genes. showed lower Cd accumulation and lower tolerance, with more pronounced PCD, compared with . Our results provide new insights into the mechanism that GABA enhances Cd tolerance in plants.

References

Chen Z, Feng Y, Guo Z, Han M, Yan X . Zinc oxide nanoparticles alleviate cadmium toxicity and promote tolerance by modulating programmed cell death in alfalfa (Medicago sativa L.). J Hazard Mater. 2024; 469:133917. DOI: 10.1016/j.jhazmat.2024.133917. View

Chen Y, Cui J, Yang J, Zhang Z, Yuan M, Song C . Biomonitoring heavy metal contaminations by moss visible parameters. J Hazard Mater. 2015; 296:201-209. DOI: 10.1016/j.jhazmat.2015.04.060. View

Cui W, Wang H, Song J, Cao X, Rogers H, Francis D . Cell cycle arrest mediated by Cd-induced DNA damage in Arabidopsis root tips. Ecotoxicol Environ Saf. 2017; 145:569-574. DOI: 10.1016/j.ecoenv.2017.07.074. View

Kampfenkel K, Van Montagu M, Inze D . Extraction and determination of ascorbate and dehydroascorbate from plant tissue. Anal Biochem. 1995; 225(1):165-7. DOI: 10.1006/abio.1995.1127. View

Guo B, Liang Y, Zhu Y . Does salicylic acid regulate antioxidant defense system, cell death, cadmium uptake and partitioning to acquire cadmium tolerance in rice?. J Plant Physiol. 2008; 166(1):20-31. DOI: 10.1016/j.jplph.2008.01.002. View

Pattanayak G, Venkataramani S, Hortensteiner S, Kunz L, Christ B, Moulin M . Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis. Plant J. 2011; 69(4):589-600. PMC: 3274588. DOI: 10.1111/j.1365-313X.2011.04814.x. View

Yuan S, Lin H . Role of salicylic acid in plant abiotic stress. Z Naturforsch C J Biosci. 2008; 63(5-6):313-20. DOI: 10.1515/znc-2008-5-601. View

Krzyzak J, Rusinowski S, Szada-Borzyszkowska A, Pogrzeba M, Stec R, Janota P . A novel agrosinters support growth, photosynthetic efficiency and reduce trace metal elements accumulation in oilseed rape growing on metalliferous soil. Environ Pollut. 2024; 363(Pt 1):125095. DOI: 10.1016/j.envpol.2024.125095. View

Dai S, Wang B, Song Y, Xie Z, Li C, Li S . Astaxanthin and its gold nanoparticles mitigate cadmium toxicity in rice by inhibiting cadmium translocation and uptake. Sci Total Environ. 2021; 786:147496. DOI: 10.1016/j.scitotenv.2021.147496. View

10.

Jiang H, Zhang Y, Li J, Tang R, Liang F, Tang R . Genome-wide identification of SIMILAR to RCD ONE (SRO) gene family in rapeseed ( L.) reveals their role in drought stress response. Plant Signal Behav. 2024; 19(1):2379128. PMC: 11249032. DOI: 10.1080/15592324.2024.2379128. View

11.

Huang G, Ding C, Li Y, Zhang T, Wang X . Selenium enhances iron plaque formation by elevating the radial oxygen loss of roots to reduce cadmium accumulation in rice (Oryza sativa L.). J Hazard Mater. 2020; 398:122860. DOI: 10.1016/j.jhazmat.2020.122860. View

12.

Ye Y, Li Z, Xing D . Sorting out the role of nitric oxide in cadmium-induced Arabidopsis thaliana programmed cell death. Plant Signal Behav. 2012; 7(11):1493-4. PMC: 3548877. DOI: 10.4161/psb.21893. View

13.

Carlile G, Smith D, Wiedmann M . Caspase-3 has a nonapoptotic function in erythroid maturation. Blood. 2004; 103(11):4310-6. DOI: 10.1182/blood-2003-09-3362. View

14.

Li Z, Ding B, Zhou X, Wang G . The Rice Dynamin-Related Protein OsDRP1E Negatively Regulates Programmed Cell Death by Controlling the Release of Cytochrome c from Mitochondria. PLoS Pathog. 2017; 13(1):e1006157. PMC: 5266325. DOI: 10.1371/journal.ppat.1006157. View

15.

Liang X, Qian R, Ou Y, Wang D, Lin X, Sun C . Lipid peroxide-derived short-chain aldehydes promote programmed cell death in wheat roots under aluminum stress. J Hazard Mater. 2022; 443(Pt A):130142. DOI: 10.1016/j.jhazmat.2022.130142. View

16.

Zhao Y, Song X, Zhong D, Yu L, Yu X . γ-Aminobutyric acid (GABA) regulates lipid production and cadmium uptake by Monoraphidium sp. QLY-1 under cadmium stress. Bioresour Technol. 2019; 297:122500. DOI: 10.1016/j.biortech.2019.122500. View

17.

Wang G, Kong J, Cui D, Zhao H, Niu Y, Xu M . Resistance against Ralstonia solanacearum in tomato depends on the methionine cycle and the γ-aminobutyric acid metabolic pathway. Plant J. 2018; 97(6):1032-1047. DOI: 10.1111/tpj.14175. View

18.

Lombardi L, Ceccarelli N, Picciarelli P, Lorenzi R . DNA degradation during programmed cell death in Phaseolus coccineus suspensor. Plant Physiol Biochem. 2007; 45(3-4):221-7. DOI: 10.1016/j.plaphy.2007.01.014. View

19.

Supel P, Sliwa-Cebula M, Miszalski Z, Kaszycki P . Cadmium-Tolerant Rhizospheric Bacteria of the C/CAM Intermediate Semi-Halophytic Common Ice Plant ( L.) Grown in Contaminated Soils. Front Plant Sci. 2022; 13:820097. PMC: 8957870. DOI: 10.3389/fpls.2022.820097. View

20.

Li Y, Li Y, Cui Y, Xie Y, Shi Y, Shang Y . GABA-mediated inhibition of cadmium uptake and accumulation in apples. Environ Pollut. 2022; 300:118867. DOI: 10.1016/j.envpol.2022.118867. View