Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry

Overview

Journal Molecules

Publisher MDPI

Date 2025 Jan 11

PMID 39795196

Authors

Tomasz Tuzimski

Szymon Szubartowski

Janusz Stazka

Kamil Baczewski

Daria Janiszewska

Viorica Railean

Boguslaw Buszewski

Malgorzata Szultka-Mlynska

Affiliations

Soon will be listed here.

Abstract

Bisphenols may negatively impact human health. In this study, we propose the use of HPLC-FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and "green" DLLME method was used, achieving mean recovery values in the range of 62%-98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC-MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time.

References

Sosa E, Scanavacca M, Davila A, Pilleggi F . A new technique to perform epicardial mapping in the electrophysiology laboratory. J Cardiovasc Electrophysiol. 1996; 7(6):531-6. DOI: 10.1111/j.1540-8167.1996.tb00559.x. View

Bocato M, Aparecida Cesila C, Lataro B, de Oliveira A, Campiglia A, Barbosa Jr F . A fast-multiclass method for the determination of 21 endocrine disruptors in human urine by using vortex-assisted dispersive liquid-liquid microextraction (VADLLME) and LC-MS/MS. Environ Res. 2020; 189:109883. DOI: 10.1016/j.envres.2020.109883. View

Dualde P, Pardo O, Fernandez S, Pastor A, Yusa V . Determination of four parabens and bisphenols A, F and S in human breast milk using QuEChERS and liquid chromatography coupled to mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2019; 1114-1115:154-166. DOI: 10.1016/j.jchromb.2019.03.004. View

Sullivan A, Dennis A, Rathod K, Jones D, Rosmini S, Manisty C . Pericardial Fluid Analysis in Diagnosis and Prognosis of Patients Who Underwent Pericardiocentesis. Am J Cardiol. 2023; 198:79-87. DOI: 10.1016/j.amjcard.2023.04.034. View

Tuzimski T, Szubartowski S, Stupak A, Kwasniewski W, Szultka-Mlynska M, Kwasniewska A . The Association between the Bisphenols Residues in Amniotic Fluid and Fetal Abnormalities in Polish Pregnant Women-Its Potential Clinical Application. Int J Mol Sci. 2023; 24(1). PMC: 9821541. DOI: 10.3390/ijms24010730. View

Contreras M, Gonzalez M, Gonzalez S, Ventura R, Valverde J, Hernandez A . Validation of a procedure for the gas chromatography-mass spectrometry analysis of cocaine and metabolites in pericardial fluid. J Anal Toxicol. 2007; 31(2):75-80. DOI: 10.1093/jat/31.2.75. View

Rezaee M, Assadi Y, Hosseini M, Aghaee E, Ahmadi F, Berijani S . Determination of organic compounds in water using dispersive liquid-liquid microextraction. J Chromatogr A. 2006; 1116(1-2):1-9. DOI: 10.1016/j.chroma.2006.03.007. View

Tuzimski T, Szubartowski S, Gadzala-Kopciuch R, Miturski A, Wojtowicz-Marzec M, Kwasniewski W . Comparison of DAD and FLD Detection for Identification of Selected Bisphenols in Human Breast Milk Samples and Their Quantitative Analysis by LC-MS/MS. J AOAC Int. 2020; 103(4):1029-1042. PMC: 8370428. DOI: 10.1093/jaoacint/qsaa027. View

Shivkumar K . Catheter Ablation of Ventricular Arrhythmias. N Engl J Med. 2019; 380(16):1555-1564. DOI: 10.1056/NEJMra1615244. View

10.

Fellahi J, Leger P, Philippe E, Arthaud M, Riou B, Gandjbakhch I . Pericardial cardiac troponin I release after coronary artery bypass grafting. Anesth Analg. 1999; 89(4):829-34. DOI: 10.1097/00000539-199910000-00004. View

11.

Valderrabano M, Cesario D, Ji S, Shannon K, Wiener I, Swerdlow C . Percutaneous epicardial mapping during ablation of difficult accessory pathways as an alternative to cardiac surgery. Heart Rhythm. 2005; 1(3):311-6. DOI: 10.1016/j.hrthm.2004.03.073. View

12.

Ranciere F, Lyons J, Loh V, Botton J, Galloway T, Wang T . Bisphenol A and the risk of cardiometabolic disorders: a systematic review with meta-analysis of the epidemiological evidence. Environ Health. 2015; 14:46. PMC: 4472611. DOI: 10.1186/s12940-015-0036-5. View

13.

Zhang B, He Y, Zhu H, Huang X, Bai X, Kannan K . Concentrations of bisphenol A and its alternatives in paired maternal-fetal urine, serum and amniotic fluid from an e-waste dismantling area in China. Environ Int. 2020; 136:105407. DOI: 10.1016/j.envint.2019.105407. View

14.

Szubartowski S, Tuzimski T . Application of High-Performance Liquid Chromatography Combined with Fluorescence Detector and Dispersive Liquid-Liquid Microextraction to Quantification of Selected Bisphenols in Human Amniotic Fluid Samples. Int J Environ Res Public Health. 2023; 20(1). PMC: 9819170. DOI: 10.3390/ijerph20010297. View

15.

Shivkumar K . Percutaneous epicardial ablation of atrial fibrillation. Heart Rhythm. 2007; 5(1):152-4. DOI: 10.1016/j.hrthm.2007.08.033. View

16.

Rocha B, de Oliveira A, Barbosa Jr F . A fast and simple air-assisted liquid-liquid microextraction procedure for the simultaneous determination of bisphenols, parabens, benzophenones, triclosan, and triclocarban in human urine by liquid chromatography-tandem mass spectrometry. Talanta. 2018; 183:94-101. DOI: 10.1016/j.talanta.2018.02.052. View

17.

Zhou J, Chen X, Zhang D, Jin M, Zhuang L, Du Y . Determination of multiple bisphenol analogues and their metabolites in human serum by liquid chromatography tandem mass spectrometry. Environ Pollut. 2022; 312:120092. DOI: 10.1016/j.envpol.2022.120092. View

18.

Vela-Soria F, Jimenez-Diaz I, Diaz C, Perez J, Iribarne-Duran L, Serrano-Lopez L . Determination of endocrine-disrupting chemicals in human milk by dispersive liquid-liquid microextraction. Bioanalysis. 2016; 8(17):1777-91. DOI: 10.4155/bio-2016-0073. View

19.

Tambara K, Fujita M, Miyamoto S, Doi K, Nishimura K, Komeda M . Pericardial fluid level of heart-type cytoplasmic fatty acid-binding protein (H-FABP) is an indicator of severe myocardial ischemia. Int J Cardiol. 2004; 93(2-3):281-4. DOI: 10.1016/S0167-5273(03)00219-5. View

20.

Alerhand S, Adrian R, Long B, Avila J . Pericardial tamponade: A comprehensive emergency medicine and echocardiography review. Am J Emerg Med. 2022; 58:159-174. DOI: 10.1016/j.ajem.2022.05.001. View