NtLPA1 Overexpression Regulates the Growth of Tobacco and Enhances Resistance to Blight

Overview

Journal Transgenic Res

Date 2025 Jan 9

PMID 39786624

Authors

Guiqin Shi

Yanxiao Bu

Lei Chi

Xifeng Zhang

Yuqing Meng

Shijie Zhang

Geng Tian

Affiliations

Soon will be listed here.

Abstract

The involvement of Loose Plant Architecture 1 (LPA1) in regulating plant growth and leaf angle has been previously demonstrated. However, the fundamental genetic background remains unidentified. To further understand the tissue expression profile of the NtLPA1 gene, an overexpression vector (pBI121-NtLPA1) was developed and employed to modify tobacco using the leaf disc method genetically. Validation confirmed the generation of transgenic tobacco plants with NtLPA1 overexpression. The findings indicated that increased NtLPA1 overexpression substantially decreased plant auxin sensitivity and modulated signal transduction and polar transport, significantly reducing leaf angle, diminished leaf area during early and late growth stages, and shortened root length. In summary, NtLPA1 augmented tobacco resistance to severe shin disease by modulating the expression of disease-associated genes PBZ1, PR1b, and the growth regulator auxin polar transport factor PIN1.

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