Molecular Markers in Cell Cycle Visualisation During Development and Stress Conditions in

Overview

Journal Quant Plant Biol

Date 2025 Jan 8

PMID 39777029

Authors

Olivia S Hazelwood

M Arif Ashraf

Affiliations

Soon will be listed here.

Abstract

Plant growth and development are tightly regulated by cell division, elongation, and differentiation. A visible plant phenotype at the tissue or organ level is coordinated at the cellular level. Among these cellular regulations (cell division, elongation and differentiation), cell division in plants follows the same universal mechanisms across kingdoms of life, and involves conserved cell cycle regulatory proteins (cyclins, cyclin-dependent kinase and cell cycle inhibitors). Cell division is regulated through distinct cell cycle steps (G1, S, G2 and M), and these individual steps are visualised using transgenic marker lines. As a result, a quantitative cell cycle approach in plants during development and stress conditions relies on the accuracy of cell cycle markers. In this perspective article, we highlight the available cell cycle marker lines in plants, common practices within plant biology communities based on existing literature and provide a road map to a thorough quantitative approach of cell cycle regulation in plants.

References

Halat L, Gyte K, Wasteneys G . The Microtubule-Associated Protein CLASP Is Translationally Regulated in Light-Dependent Root Apical Meristem Growth. Plant Physiol. 2020; 184(4):2154-2167. PMC: 7723079. DOI: 10.1104/pp.20.00474. View

Uzbekov R, Prigent C . A Journey through Time on the Discovery of Cell Cycle Regulation. Cells. 2022; 11(4). PMC: 8870340. DOI: 10.3390/cells11040704. View

Wang Z, Wang X, Xie B, Hong Z, Yang Q . Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) regulates cell cycling potentially by cooperating with nucleosome assembly protein AtNAP1;1. BMC Plant Biol. 2018; 18(1):99. PMC: 5984758. DOI: 10.1186/s12870-018-1289-2. View

Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H . Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 2008; 132(3):487-98. DOI: 10.1016/j.cell.2007.12.033. View

Nishitani H, Lygerou Z, Nishimoto T, Nurse P . The Cdt1 protein is required to license DNA for replication in fission yeast. Nature. 2000; 404(6778):625-8. DOI: 10.1038/35007110. View

Caicedo J, Goodman A, Karhohs K, Cimini B, Ackerman J, Haghighi M . Nucleus segmentation across imaging experiments: the 2018 Data Science Bowl. Nat Methods. 2019; 16(12):1247-1253. PMC: 6919559. DOI: 10.1038/s41592-019-0612-7. View

Benmaamar R, Pagano M . Involvement of the SCF complex in the control of Cdh1 degradation in S-phase. Cell Cycle. 2005; 4(9):1230-2. DOI: 10.4161/cc.4.9.2048. View

Reid B, Culotti J, Nash R, Pringle J . Forty-five years of cell-cycle genetics. Mol Biol Cell. 2015; 26(24):4307-12. PMC: 4666127. DOI: 10.1091/mbc.E14-10-1484. View

Olatunji D, Clark N, Kelley D . The class VIII myosin ATM1 is required for root apical meristem function. Development. 2023; 150(20). PMC: 10357013. DOI: 10.1242/dev.201762. View

10.

Buschmann H, Holtmannspotter M, Borchers A, ODonoghue M, Zachgo S . Microtubule dynamics of the centrosome-like polar organizers from the basal land plant Marchantia polymorpha. New Phytol. 2015; 209(3):999-1013. DOI: 10.1111/nph.13691. View

11.

Maga G, Hubscher U . Proliferating cell nuclear antigen (PCNA): a dancer with many partners. J Cell Sci. 2003; 116(Pt 15):3051-60. DOI: 10.1242/jcs.00653. View

12.

Ubeda-Tomas S, Federici F, Casimiro I, Beemster G, Bhalerao R, Swarup R . Gibberellin signaling in the endodermis controls Arabidopsis root meristem size. Curr Biol. 2009; 19(14):1194-9. DOI: 10.1016/j.cub.2009.06.023. View

13.

Allsman L, Bellinger M, Huang V, Duong M, Contreras A, Romero A . Subcellular positioning during cell division and cell plate formation in maize. Front Plant Sci. 2023; 14:1204889. PMC: 10360171. DOI: 10.3389/fpls.2023.1204889. View

14.

Moldovan G, Pfander B, Jentsch S . PCNA, the maestro of the replication fork. Cell. 2007; 129(4):665-79. DOI: 10.1016/j.cell.2007.05.003. View

15.

Schnittger A, De Veylder L . The Dual Face of Cyclin B1. Trends Plant Sci. 2018; 23(6):475-478. DOI: 10.1016/j.tplants.2018.03.015. View

16.

Wolpert L . The evolution of 'the cell theory'. Curr Biol. 1996; 6(3):225-8. DOI: 10.1016/s0960-9822(02)00463-3. View

17.

Desvoyes B, Arana-Echarri A, Barea M, Gutierrez C . A comprehensive fluorescent sensor for spatiotemporal cell cycle analysis in Arabidopsis. Nat Plants. 2020; 6(11):1330-1334. DOI: 10.1038/s41477-020-00770-4. View

18.

Caro E, Gutierrez C . A green GEM: intriguing analogies with animal geminin. Trends Cell Biol. 2007; 17(12):580-5. DOI: 10.1016/j.tcb.2007.09.008. View

19.

Kutashev K, Meschichi A, Reeck S, Fonseca A, Sartori K, White C . Differences in RAD51 transcriptional response and cell cycle dynamics reveal varying sensitivity to DNA damage among Arabidopsis thaliana root cell types. New Phytol. 2024; 243(3):966-980. DOI: 10.1111/nph.19875. View

20.

Echevarria C, Gutierrez C, Desvoyes B . Tools for Assessing Cell-Cycle Progression in Plants. Plant Cell Physiol. 2021; 62(8):1231-1238. PMC: 8579159. DOI: 10.1093/pcp/pcab066. View