Complete Mitochondrial Genomes of and Biting Midge Vectors of Bluetongue Virus

Biting midges ( spp.) are important vectors of several insect borne arboviruses but are underrepresented in terms of availability of high-resolution genomic resources. We assembled and annotated complete mitochondrial genomes for two species, namely and which are proven vectors for Bluetongue Virus (BTV). We used both long read and short read sequencing technologies to assemble the circular genomes. The genome sizes are 17,100 bp and 17,031 bp, respectively, all comprising 37 genes, including 13 protein, 22 tRNA, two rRNA coding genes, and one non-coding AT rich control region. The gene organizations and orientations are comparable to other available mitogenomes, except for a translocation of and six tRNA genes in both and . Eleven protein-coding genes encode a full TAA stop codon, while two (, ) are completed by mRNA polyadenylation. Phylogenetic analysis of the mitogenomes showed and form a monophyletic group. The sequences of these mitogenomes contribute to a baseline of molecular tools for diagnostics and surveillance for use by World Organisation for Animal Health (WOAH) reference laboratories for monitoring vectors of emerging diseases.