Sarcocystis Cymruensis in the Brown Rat (Rattus Norvegicus) from an Urban District in Kuwait: Detailed Morphologic and Molecular Characterization

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2025 Jan 8

PMID 39775129

Authors

Fatemah A M Aryan

Petras Prakas

Evelina Juozaityte-Ngugu

Donatas Sneideris

Zein Abd-Al-Aal

Abd Allah A Alhoot

Amira I El-Kabbany

Laila M A Tahrani

Osama M E El-Azazy

Affiliations

Soon will be listed here.

Abstract

Purpose: The objective of the study was to establish the prevalence of Sarcocystis (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.

Methods: Ninety-eight brown rats (Rattus norvegicus) were examined for Sarcocystis spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy. The detected Sarcocystis species was characterised at nuclear 18S and 28S ribosomal RNA (rRNA), internal transcribed spacers 1 and 2, (ITS1 and ITS2), mitochondrial cytochrome c oxidase subunit I (cox1) and cytochrome b (cytb), and apicoplast RNA polymerase beta subunit (rpoB).

Results: Sarcocysts were found in thigh muscles of 13.3% of the animals examined, while no oocysts/sporocysts were detected in faecal samples. Under a light microscope, sarcocysts were spindle-shaped, 850-3152 × 73-125 μm (1781 ± 763 × 99 ± 15 μm) in size and had thin (up to 0.9 μm) and apparently smooth cyst wall. By transmission electron microscopy, sarcocyst wall was 0.7-1.1 μm in thickness with numerous osmiophilic bleb-like protrusions. Based on DNA sequencing the sarcocysts examined were identified as S. cymruensis. Notably, ITS2 and rpoB sequences of S. cymruensis were obtained for the first time. No intraspecific variation was detected comparing 28S rRNA, ITS1, cox1 and cytb sequences of S. cymruensis isolated from Kuwait, Grenada and China. According to phylogenetic analysis, S. cymruensis was most closely related to S. muris, S. myodes and S. ratti using rodents as their intermediate hosts and cats as their identified or predatory mammals as their presumed definitive hosts.

Conclusion: We present the first report of S. cymruensis in Kuwait and in Persian Gulf area. The study shed light on the usefulness of different genetic loci for the characterization of Sarcocystis spp. from rodents.

Citing Articles

Morphological and molecular characterisation of Sarcocystis capracanis, Sarcocystis cornagliai and Sarcocystis rossii n. sp. infecting the Alpine ibex (Capra ibex).

Rudaityte-Lukosiene E, Rehbein S, Calero-Bernal R, Butkauskas D, Prakas P Parasit Vectors. 2025; 18(1):96.

PMID: 40065382 PMC: 11892209. DOI: 10.1186/s13071-025-06737-8.

References

Pacsa A, Elbishbishi E, Chaturvedi U, Chu K, Mustafa A . Hantavirus-specific antibodies in rodents and humans living in Kuwait. FEMS Immunol Med Microbiol. 2002; 33(2):139-42. DOI: 10.1111/j.1574-695X.2002.tb00583.x. View

Mehlhorn H, Heydorn A . The sarcosporidia (Protozoa, Sporozoa): life cycle and fine structure. Adv Parasitol. 1978; 16:43-91. DOI: 10.1016/s0065-308x(08)60572-2. View

Ashford R . Sarcocystis cymruensis n. sp., a parasite of rats Rattus norvegicus and cats Felis catus. Ann Trop Med Parasitol. 1978; 72(1):37-43. DOI: 10.1080/00034983.1978.11719277. View

Zaman V, Colley F . Light and electron microscopic observations of the life cycle of Sarcocystis orientalis sp. n. in the rat (Rattus norvegicus) and the Malaysian reticulated python (Python reticulatus). Z Parasitenkd. 1975; 47(3):169-85. DOI: 10.1007/BF00418200. View

Munday B . An isosporan parasite of masked owls producing sarcocysts in rats. J Wildl Dis. 1983; 19(2):146-7. DOI: 10.7589/0090-3558-19.2.146. View

Hu J, Meng Y, Guo Y, Liao J, Song J . Completion of the life cycle of Sarcocystis zuoi , a parasite from the Norway rat, Rattus norvegicus. J Parasitol. 2011; 98(3):550-3. DOI: 10.1645/GE-2831.1. View

Prakas P, Kirillova V, Gavarane I, Gravele E, Butkauskas D, Rudaityte-Lukosiene E . Morphological and molecular description of Sarcocystis ratti n. sp. from the black rat (Rattus rattus) in Latvia. Parasitol Res. 2019; 118(9):2689-2694. DOI: 10.1007/s00436-019-06393-9. View

Zeng H, Guo Y, Ma C, Deng S, Hu J, Zhang Y . Redescription and molecular characterization of sarcocysts of Sarcocystis cymruensis from Norway rats (Rattus norvegicus) and Sarcocystis ratti from black rats (R. rattus) in China. Parasitol Res. 2020; 119(11):3785-3791. DOI: 10.1007/s00436-020-06883-1. View

Jakel T, Raisch L, Richter S, Wirth M, Birenbaum D, Ginting S . Morphological and molecular phylogenetic characterization of sp. nov. and other novel, closely related spp. infecting small mammals and colubrid snakes in Asia. Int J Parasitol Parasites Wildl. 2023; 22:184-198. PMC: 10615900. DOI: 10.1016/j.ijppaw.2023.10.005. View

10.

Qin T, Ortega-Perez P, Wibbelt G, Lakim M, Ginting S, Khoprasert Y . A cyst-forming coccidian with large geographical range infecting forest and commensal rodents: Sarcocystis muricoelognathis sp. nov. Parasit Vectors. 2024; 17(1):135. PMC: 10943850. DOI: 10.1186/s13071-024-06230-8. View

11.

Abu-Madi M, Behnke J, Mikhail M, Lewis J, Al-Kaabi M . Parasite populations in the brown rat Rattus norvegicus from Doha, Qatar between years: the effect of host age, sex and density. J Helminthol. 2005; 79(2):105-11. DOI: 10.1079/joh2005274. View

12.

Dryden M, Payne P, Ridley R, Smith V . Comparison of common fecal flotation techniques for the recovery of parasite eggs and oocysts. Vet Ther. 2005; 6(1):15-28. View

13.

Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth B, Remm M . Primer3--new capabilities and interfaces. Nucleic Acids Res. 2012; 40(15):e115. PMC: 3424584. DOI: 10.1093/nar/gks596. View

14.

Kutkiene L, Prakas P, Sruoga A, Butkauskas D . The mallard duck (Anas platyrhynchos) as intermediate host for Sarcocystis wobeseri sp. nov. from the barnacle goose (Branta leucopsis). Parasitol Res. 2010; 107(4):879-88. DOI: 10.1007/s00436-010-1945-4. View

15.

Gjerde B . Molecular characterisation of Sarcocystis rileyi from a common eider (Somateria mollissima) in Norway. Parasitol Res. 2014; 113(9):3501-9. DOI: 10.1007/s00436-014-4062-y. View

16.

Gjerde B . Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol. 2013; 43(7):579-91. DOI: 10.1016/j.ijpara.2013.02.004. View

17.

Hu J, Sun J, Guo Y, Zeng H, Zhang Y, Tao J . Infection of the Asian gray shrew Crocidura attenuata (Insectivora: Soricidae) with Sarcocystis attenuati n. sp. (Apicomplexa: Sarcocystidae) in China. Parasit Vectors. 2022; 15(1):13. PMC: 8751003. DOI: 10.1186/s13071-021-05136-z. View

18.

Wendte J, Miller M, Lambourn D, Magargal S, Jessup D, Grigg M . Self-mating in the definitive host potentiates clonal outbreaks of the apicomplexan parasites Sarcocystis neurona and Toxoplasma gondii. PLoS Genet. 2011; 6(12):e1001261. PMC: 3009688. DOI: 10.1371/journal.pgen.1001261. View

19.

Altschul S, Gish W, Miller W, Myers E, Lipman D . Basic local alignment search tool. J Mol Biol. 1990; 215(3):403-10. DOI: 10.1016/S0022-2836(05)80360-2. View

20.

Mugridge N, Morrison D, Heckeroth A, Johnson A, Tenter A . Phylogenetic analysis based on full-length large subunit ribosomal RNA gene sequence comparison reveals that Neospora caninum is more closely related to Hammondia heydorni than to Toxoplasma gondii. Int J Parasitol. 1999; 29(10):1545-56. DOI: 10.1016/s0020-7519(99)00150-2. View