Harnessing of Sunflower Stalks by Hydrolysis and Fermentation with to Produce Biofuels

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2025 Jan 8

PMID 39771400

Authors

MA Lourdes Martinez-Cartas

Manuel Cuevas-Aranda

Sebastian Sanchez

Affiliations

Soon will be listed here.

Abstract

A sequential valorization process of sunflower stalks was carried out using nitric acid (0.1-2 mol dm) as a hydrolytic agent and fermenting the hydrolysate of higher sugar concentration in the presence of the non-conventional yeast . Values reached for ethanol yield (0.25 g g) and xylitol yield (0.14 g g) were higher than those achieved after pretreatment with other acids in previous studies. The effect of acid treatment with nitric, phosphoric, and sulfuric acids on the separated solid fractions was evaluated to determine its potential use as solid biofuel by FTIR and SEM determinations. A significant loss of lignin and hemicellulose was found in the solid treated with nitric acid, while a higher HHV was obtained when pretreated with phosphoric acid (19.16 MJ kg) and sulfuric acid (19.12 MJ kg). A subsequent enzymatic hydrolysis of acid-pretreated solids showed that the nitric acid pretreatment increased the availability of glucose from the cellulose fraction to a greater extent than the other two acids, by reducing the hemicellulose fraction to 0.7% and the lignin fraction to 2.5%. This study shows that pretreatment of biomass with nitric acid leads to better fermentation results to obtain biofuels such as ethanol, which could be further increased by additional enzymatic hydrolysis, while pretreatment with the other two acids generates better solid fuels.

References

Li X, Chen Y, Nielsen J . Harnessing xylose pathways for biofuels production. Curr Opin Biotechnol. 2019; 57:56-65. DOI: 10.1016/j.copbio.2019.01.006. View

Ruchala J, Kurylenko O, Dmytruk K, Sibirny A . Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha). J Ind Microbiol Biotechnol. 2019; 47(1):109-132. PMC: 6970964. DOI: 10.1007/s10295-019-02242-x. View

Akerholm M, Hinterstoisser B, Salmen L . Characterization of the crystalline structure of cellulose using static and dynamic FT-IR spectroscopy. Carbohydr Res. 2004; 339(3):569-78. DOI: 10.1016/j.carres.2003.11.012. View

Mills T, Sandoval N, Gill R . Cellulosic hydrolysate toxicity and tolerance mechanisms in Escherichia coli. Biotechnol Biofuels. 2009; 2:26. PMC: 2770041. DOI: 10.1186/1754-6834-2-26. View

Radecka D, Mukherjee V, Mateo R, Stojiljkovic M, Foulquie-Moreno M, Thevelein J . Looking beyond Saccharomyces: the potential of non-conventional yeast species for desirable traits in bioethanol fermentation. FEMS Yeast Res. 2015; 15(6). DOI: 10.1093/femsyr/fov053. View

Mao J, Holtman K, Franqui-Villanueva D . Chemical structures of corn stover and its residue after dilute acid prehydrolysis and enzymatic hydrolysis: insight into factors limiting enzymatic hydrolysis. J Agric Food Chem. 2010; 58(22):11680-7. DOI: 10.1021/jf102514r. View

LINDEGREN C, NAGAI S, Nagai H . Induction of respiratory deficiency in yeast by manganese, copper, cobalt and nickel. Nature. 1958; 182(4633):446-8. DOI: 10.1038/182446a0. View

Galafassi S, Capusoni C, Moktaduzzaman M, Compagno C . Utilization of nitrate abolishes the "Custers effect" in Dekkera bruxellensis and determines a different pattern of fermentation products. J Ind Microbiol Biotechnol. 2013; 40(3-4):297-303. DOI: 10.1007/s10295-012-1229-3. View

Schmidt F . [Enzymatic determination of glucose and fructose simultaneously]. Klin Wochenschr. 1961; 39:1244-7. DOI: 10.1007/BF01506150. View

10.

Cardona C, Sanchez O . Fuel ethanol production: process design trends and integration opportunities. Bioresour Technol. 2007; 98(12):2415-57. DOI: 10.1016/j.biortech.2007.01.002. View

11.

Cockerill S, Martin C . Are biofuels sustainable? The EU perspective. Biotechnol Biofuels. 2008; 1(1):9. PMC: 2390541. DOI: 10.1186/1754-6834-1-9. View

12.

Bratiichuk D, Kurylenko O, Vasylyshyn R, Zuo M, Kang Y, Dmytruk K . Development of new dominant selectable markers for the nonconventional yeasts Ogataea polymorpha and Candida famata. Yeast. 2020; 37(9-10):505-513. DOI: 10.1002/yea.3467. View

13.

Jonsson L, Martin C . Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects. Bioresour Technol. 2015; 199:103-112. DOI: 10.1016/j.biortech.2015.10.009. View

14.

Kurylenko O, Ruchala J, Hryniv O, Abbas C, Dmytruk K, Sibirny A . Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation. Microb Cell Fact. 2014; 13:122. PMC: 4145226. DOI: 10.1186/s12934-014-0122-3. View

15.

Kurylenko O, Ruchala J, Kruk B, Vasylyshyn R, Szczepaniak J, Dmytruk K . The role of Mig1, Mig2, Tup1 and Hap4 transcription factors in regulation of xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha. FEMS Yeast Res. 2021; 21(4). DOI: 10.1093/femsyr/foab029. View

16.

Manfrao-Netto J, Gomes A, Skorupa Parachin N . Advances in Using as Chassis for Recombinant Protein Production. Front Bioeng Biotechnol. 2019; 7:94. PMC: 6504786. DOI: 10.3389/fbioe.2019.00094. View

17.

Cheng H, Whang L . Resource recovery from lignocellulosic wastes via biological technologies: Advancements and prospects. Bioresour Technol. 2021; 343:126097. DOI: 10.1016/j.biortech.2021.126097. View

18.

Woiciechowski A, Neto C, Porto de Souza Vandenberghe L, de Carvalho Neto D, Sydney A, Junior Letti L . Lignocellulosic biomass: Acid and alkaline pretreatments and their effects on biomass recalcitrance - Conventional processing and recent advances. Bioresour Technol. 2020; 304:122848. DOI: 10.1016/j.biortech.2020.122848. View

19.

Keating J, Panganiban C, Mansfield S . Tolerance and adaptation of ethanologenic yeasts to lignocellulosic inhibitory compounds. Biotechnol Bioeng. 2006; 93(6):1196-206. DOI: 10.1002/bit.20838. View

20.

Martinez-Cartas M, Olivares M, Sanchez S . Production of bioalcohols and antioxidant compounds by acid hydrolysis of lignocellulosic wastes and fermentation of hydrolysates with . Eng Life Sci. 2020; 19(7):522-536. PMC: 6999450. DOI: 10.1002/elsc.201900011. View