Experimental Evaluation of the Effect of Degradation on the Mechanical Behavior and Morphometric Characteristics of Functionally Graded Polymer Scaffolds

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2025 Jan 8

PMID 39771326

Authors

Nataliya Elenskaya

Ilia Vindokurov

Evgeniy Sadyrin

Andrey Nikolaev

Mikhail Tashkinov

Affiliations

Soon will be listed here.

Abstract

Bone transplantation ranks second worldwide among tissue prosthesis surgeries. Currently, one of the most promising approaches is regenerative medicine, which involves tissue engineering based on polymer scaffolds with biodegradable properties. Once implanted, scaffolds interact directly with the surrounding tissues and in a fairly aggressive environment, which causes biodegradation of the scaffold material. The aim of this work is to experimentally investigate the changes in the effective mechanical properties of polylactide scaffolds manufactured using additive technologies. The mechanism and the rate of the degradation process depend on the chosen material, contact area, microstructural features, and overall architecture of sample. To assess the influence of each of these factors, solid samples with different dimensions and layers orientation as well as prototypes of functionally graded scaffolds were studied. The research methodology includes the assessment of changes in the mechanical properties of the samples, as well as their structural characteristics. Changes in the mechanical properties were measured in compression tests. Microcomputed tomography (micro-CT) studies were conducted to evaluate changes in the microstructure of scaffold prototypes. Changes caused by surface erosion and their impact on degradation were assessed using morphometric analysis. Nonlinear changes in mechanical properties were observed for both solid samples and lattice graded scaffold prototypes depending on the duration of immersion in NaCl solution and exposure to different temperatures. At the temperature of 37 °C, the decrease in the elastic modulus of solid specimens was no more than 16%, while for the lattice scaffolds, it was only 4%. For expedited degradation during a higher temperature of 45 °C, these ratios were 47% and 16%, respectively. The decrease in compressive strength was no more than 32% for solid specimens and 17% for scaffolds. The results of this study may be useful for the development of optimal scaffolds considering the impact of the degradation process on their structural integrity.

Citing Articles

Development of Recombinant Human Collagen-Based Porous Scaffolds for Skin Tissue Engineering: Enhanced Mechanical Strength and Biocompatibility.

Yang Y, Yu T, Tao M, Wang Y, Yao X, Zhu C Polymers (Basel). 2025; 17(3).

PMID: 39940505 PMC: 11820873. DOI: 10.3390/polym17030303.

References

Hosseini H, Maquer G, Zysset P . μCT-based trabecular anisotropy can be reproducibly computed from HR-pQCT scans using the triangulated bone surface. Bone. 2017; 97:114-120. DOI: 10.1016/j.bone.2017.01.016. View

Du J, Li S, Silberschmidt V . Remodelling of trabecular bone in human distal tibia: A model based on an in-vivo HR-pQCT study. J Mech Behav Biomed Mater. 2021; 119:104506. DOI: 10.1016/j.jmbbm.2021.104506. View

Schwiedrzik J, Gross T, Bina M, Pretterklieber M, Zysset P, Pahr D . Experimental validation of a nonlinear μFE model based on cohesive-frictional plasticity for trabecular bone. Int J Numer Method Biomed Eng. 2015; 32(4):e02739. DOI: 10.1002/cnm.2739. View

Poh P, Lingner T, Kalkhof S, Mardian S, Baumbach J, Dondl P . Enabling technologies towards personalization of scaffolds for large bone defect regeneration. Curr Opin Biotechnol. 2022; 74:263-270. DOI: 10.1016/j.copbio.2021.12.002. View

Nampoothiri K, Nair N, John R . An overview of the recent developments in polylactide (PLA) research. Bioresour Technol. 2010; 101(22):8493-501. DOI: 10.1016/j.biortech.2010.05.092. View

Burghardt A, Kazakia G, Sode M, de Papp A, Link T, Majumdar S . A longitudinal HR-pQCT study of alendronate treatment in postmenopausal women with low bone density: Relations among density, cortical and trabecular microarchitecture, biomechanics, and bone turnover. J Bone Miner Res. 2010; 25(12):2558-71. PMC: 3179276. DOI: 10.1002/jbmr.157. View

Lo L, Lin H . Applications of three-dimensional imaging techniques in craniomaxillofacial surgery: A literature review. Biomed J. 2023; 46(4):100615. PMC: 10339193. DOI: 10.1016/j.bj.2023.100615. View

Mustafa N, Akhmal N, Izman S, Ab Talib M, Shaiful A, Omar M . Application of Computational Method in Designing a Unit Cell of Bone Tissue Engineering Scaffold: A Review. Polymers (Basel). 2021; 13(10). PMC: 8156807. DOI: 10.3390/polym13101584. View

Winarso R, Anggoro P, Ismail R, Jamari J, Bayuseno A . Application of fused deposition modeling (FDM) on bone scaffold manufacturing process: A review. Heliyon. 2022; 8(11):e11701. PMC: 9699973. DOI: 10.1016/j.heliyon.2022.e11701. View

10.

Vafaeefar M, Moerman K, Kavousi M, Vaughan T . A morphological, topological and mechanical investigation of gyroid, spinodoid and dual-lattice algorithms as structural models of trabecular bone. J Mech Behav Biomed Mater. 2022; 138:105584. DOI: 10.1016/j.jmbbm.2022.105584. View

11.

Elenskaya N, Tashkinov M, Vindokurov I, Pirogova Y, Silberschmidt V . Understanding of trabecular-cortical transition zone: Numerical and experimental assessment of multi-morphology scaffolds. J Mech Behav Biomed Mater. 2023; 147:106146. DOI: 10.1016/j.jmbbm.2023.106146. View

12.

Elenskaya N, Koryagina P, Tashkinov M, Silberschmidt V . Effect of degradation in polymer scaffolds on mechanical properties: Surface vs. bulk erosion. Comput Biol Med. 2024; 174:108402. DOI: 10.1016/j.compbiomed.2024.108402. View

13.

Asbai-Ghoudan R, Nasello G, Perez M, Verbruggen S, Ruiz de Galarreta S, Rodriguez-Florez N . In silico assessment of the bone regeneration potential of complex porous scaffolds. Comput Biol Med. 2023; 165:107381. DOI: 10.1016/j.compbiomed.2023.107381. View

14.

Liu Y, Rath B, Tingart M, Eschweiler J . Role of implants surface modification in osseointegration: A systematic review. J Biomed Mater Res A. 2019; 108(3):470-484. DOI: 10.1002/jbm.a.36829. View

15.

Kazantseva N, Ilinikh M, Kuznetsov V, Koemets Y, Bakhrunov K, Karabanalov M . Design and Structural Factors' Influence on the Fatigue Life of Steel Products with Additive Manufacturing. Materials (Basel). 2023; 16(23). PMC: 10707552. DOI: 10.3390/ma16237315. View

16.

Zhang H, Zhou L, Zhang W . Control of scaffold degradation in tissue engineering: a review. Tissue Eng Part B Rev. 2014; 20(5):492-502. DOI: 10.1089/ten.TEB.2013.0452. View

17.

Wu L, Ding J . Effects of porosity and pore size on in vitro degradation of three-dimensional porous poly(D,L-lactide-co-glycolide) scaffolds for tissue engineering. J Biomed Mater Res A. 2005; 75(4):767-77. DOI: 10.1002/jbm.a.30487. View

18.

Smotrova E, Li S, Silberschmidt V . Mechanoregulated trabecular bone adaptation: Progress report on approaches. Biomater Biosyst. 2023; 7:100058. PMC: 9934474. DOI: 10.1016/j.bbiosy.2022.100058. View

19.

Du J, Brooke-Wavell K, Paggiosi M, Hartley C, Walsh J, Silberschmidt V . Characterising variability and regional correlations of microstructure and mechanical competence of human tibial trabecular bone: An in-vivo HR-pQCT study. Bone. 2019; 121:139-148. DOI: 10.1016/j.bone.2019.01.013. View

20.

Hussein K, Park K, Kang K, Woo H . Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application. Mater Sci Eng C Mater Biol Appl. 2016; 67:766-778. DOI: 10.1016/j.msec.2016.05.068. View