Nitrogen-Fixing and : Two Novel Species from Plant Rhizospheres

Overview

Journal Microorganisms

Specialty Microbiology

Date 2025 Jan 8

PMID 39770764

Authors

Weilong Zhang

Miao Gao

Rui Hu

Yimin Shang

Minzhi Liu

Peichun Lan

Shuo Jiao

Gehong Wei

Sanfeng Chen

Affiliations

Soon will be listed here.

Abstract

Two strains, M1 and H32 with nitrogen-fixing ability, were isolated from the rhizospheres of different plants. Genome sequence analysis showed that a (trogen ixation) gene cluster composed of nine genes () was conserved in the two strains. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strains M1 and H32 are members of the genus . Strains M1 and H32 had 97% similarity in the 16S rRNA gene sequences. Strain M1 had the highest similarity (97.25%) with LAM 0504T in the 16S rRNA gene sequences. Strain H32 had the highest similarity (97.48%) with TCIP 101062T in the 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain M1 and its closest member were 78.17% and 22.3%, respectively. ANI and dDDH values between strain H32 and its closest member were 88.94% and 66.02%, respectively. The predominant fatty acid of both strains is anteiso-C15:0. The major polar lipids of both strains are DPG (diphosphatidylglycerol) and PG (phosphatidylglycerol). The predominant isoprenoid quinone of both strains is MK-7. With all the phylogenetic and phenotypic divergency, two novel species sp. nov and sp. nov are proposed with the type strain M1 [=GDMCC (Guangdong Culture Collection Centre of Microbiology) 1.4871 = JCM (Japan Collection of Microorganisms) 37487] and with type strain H32 (=GDMCC 1.4872 = JCM37488).

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