Phage VB_SpuM_X5: A Novel Approach to Reducing Biofilms with Implications for Food Safety

Overview

Journal Microorganisms

Specialty Microbiology

Date 2025 Jan 8

PMID 39770603

Authors

Xinxin Jin

Xiuxiu Sun

Qin Lu

Zui Wang

Zhenggang Zhang

Xiaochun Ling

Yunpeng Xu

Ruiqin Liang

Junjie Yang

Li Li

Tengfei Zhang

Qingping Luo

Guofu Cheng

Affiliations

Soon will be listed here.

Abstract

, a prevalent foodborne pathogen, poses a significant social and economic strain on both food safety and public health. The application of phages in the control of foodborne pathogens represents an emerging research area. In this study, phage vB_SpuM_X5 (phage X5) was isolated from chicken farm sewage samples. The results revealed that phage X5 is a novel phage. Phage X5 has adequate temperature tolerance (28 °C-60 °C), pH stability (4-12), and a broad host range of bacteria (87.50% of tested strains). The addition of phage X5 (MOI of 100 and 1000) to milk inoculated with reduced the number of by 0.72 to 0.93 log CFU/mL and 0.66 to 1.06 log CFU/mL at 4 °C and 25 °C, respectively. The addition of phage X5 (MOI of 100 and 1000) to chicken breast inoculated with reduced bacterial numbers by 1.13 to 2.42 log CFU/mL and 0.81 to 1.25 log CFU/mL at 4 °C and 25 °C, respectively. Phage X5 has bactericidal activity against and can be used as a potential biological bacteriostatic agent to remove mature biofilms of or for the prevention and control of .

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