Genome-Wide Identification, Functional Characterization, and Stress-Responsive Expression Profiling of Subtilase () Gene Family in Peanut ( L.)

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2025 Jan 8

PMID 39769126

Authors

Shipeng Li

Huiwen Fu

Yasir Sharif

Sheidu Abdullaziz

Lihui Wang

Yongli Zhang

Yuhui Zhuang

Affiliations

Soon will be listed here.

Abstract

Subtilases (SBTs), known as serine proteases or phytoproteases in plants, are crucial enzymes involved in plant development, growth, and signaling pathways. Despite their recognized importance in other plant species, information regarding their functional roles in cultivated peanut ( L.) remains sparse. We identified 122 genes in the STQ peanut genome, classifying them into six subgroups based on phylogenetic analysis. Detailed structural and motif analyses revealed the presence of conserved domains, highlighting the evolutionary conservation of . The collinearity results indicate that the gene family has 17, 5, and 1 homologous gene pairs with , , and , respectively. Furthermore, the prediction of -elements in promoters indicates that they are mainly associated with hormones and abiotic stress. GO and KEGG analyses showed that many are important in stress response. Based on transcriptome datasets, some genes, such as , , , , , and , showed remarkably higher expression in diverse tissues/organs, i.e., embryo, root, and leaf, potentially implicating them in seed development. Likewise, only a few genes, including , , , , and , were upregulated under abiotic stress (drought and cold) and phytohormone (ethylene, abscisic acid, paclobutrazol, brassinolide, and salicylic acid) treatments. Upon inoculation with , the expression levels of , , , and were upregulated in disease-resistant and downregulated in disease-susceptible varieties. qRT-PCR-based expression profiling presented the parallel expression trends as generated from transcriptome datasets. The comprehensive dataset generated in the study provides valuable insights into understanding the functional roles of , paving the way for potential applications in crop improvement. These findings deepen our understanding of peanut molecular biology and offer new strategies for enhancing stress tolerance and other agronomically important traits.

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