In Vitro Anticancer Effects of Aqueous Leaf Extract from L. Ssp.

Overview

Journal Life (Basel)

Specialty Biology

Date 2025 Jan 8

PMID 39768248

Authors

Zlatina Gospodinova

Georgi Antov

Svetozar Stoichev

Miroslava Zhiponova

Affiliations

Soon will be listed here.

Abstract

Despite significant efforts, cancer remains the second leading cause of mortality worldwide. The medicinal plant L. represents a valuable source of biologically active compounds with pharmacological activities including antioxidant, anti-inflammatory, antimicrobial, and antiviral. This study aimed to assess the antiproliferative potential and mechanisms of action of aqueous extract from the leaves of wild-grown . Cancer cell lines, MDA-MB-231, MCF7 (breast), HT29, Colon 26 (colon), and HepG2 (liver cancer), and a non-cancerous skin cell line, BJ, were assessed for antiproliferative activity by MTT assay and observation of cell morphological alterations. The cancer cell line that was most sensitive to the extract was further studied for apoptotic alterations by Annexin V/propidium iodide staining, colony-forming assay, and qRT-PCR analysis. The results revealed that the plant extract inhibited the proliferation of all investigated cancer cell lines with the strongest cytostatic effect on Colon 26 cells with a half maximal inhibitory concentration (IC) value of 380.2 μg/mL and a selectivity index (SI) of 3.5. The extract significantly inhibited the ability of cells to form colonies, exhibited considerable proapoptotic potential involving the participation of the gene, and increased the expression levels of and the gene, which suggests a role of autophagic cell death in the antitumor action.

References

Petrova D, Gasic U, Yocheva L, Hinkov A, Yordanova Z, Chaneva G . Catmint ( L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions. Front Plant Sci. 2022; 13:866777. PMC: 9150856. DOI: 10.3389/fpls.2022.866777. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

Al-Oqail M, Al-Sheddi E, Siddiqui M, Musarrat J, Al-Khedhairy A, Farshori N . Anticancer Activity of Chloroform Extract and Sub-fractions of Nepeta deflersiana on Human Breast and Lung Cancer Cells: An In vitro Cytotoxicity Assessment. Pharmacogn Mag. 2016; 11(Suppl 4):S598-605. PMC: 4787095. DOI: 10.4103/0973-1296.172968. View

Dodurga Y, Eroglu C, Secme M, Elmas L, Biray Avci C, Lale Satiroglu-Tufan N . Anti-proliferative and anti-invasive effects of ferulic acid in TT medullary thyroid cancer cells interacting with URG4/URGCP. Tumour Biol. 2015; 37(2):1933-40. DOI: 10.1007/s13277-015-3984-z. View

Anicic N, Matekalo D, Skoric M, Gasic U, Nestorovic Zivkovic J, Dmitrovic S . Functional iridoid synthases from iridoid producing and non-producing species (subfam. Nepetoidae, fam. Lamiaceae). Front Plant Sci. 2024; 14:1211453. PMC: 10792066. DOI: 10.3389/fpls.2023.1211453. View

Rahbardar M, Amin B, Mehri S, Mirnajafi-Zadeh S, Hosseinzadeh H . Rosmarinic acid attenuates development and existing pain in a rat model of neuropathic pain: An evidence of anti-oxidative and anti-inflammatory effects. Phytomedicine. 2018; 40:59-67. DOI: 10.1016/j.phymed.2018.01.001. View

Kang R, Zeh H, Lotze M, Tang D . The Beclin 1 network regulates autophagy and apoptosis. Cell Death Differ. 2011; 18(4):571-80. PMC: 3131912. DOI: 10.1038/cdd.2010.191. View

Zahirnia A, Boroomand M, Nasirian H, Soleimani-Asl S, Salehzadeh A, Dastan D . The cytotoxicity of malathion and essential oil of (lamiales: lamiaceae) against vertebrate and invertebrate cell lines. Pan Afr Med J. 2019; 33:285. PMC: 6815490. DOI: 10.11604/pamj.2019.33.285.18776. View

Fong Y, Tang C, Hu H, Fang H, Chen B, Wu C . Inhibitory effect of -ferulic acid on proliferation and migration of human lung cancer cells accompanied with increased endogenous reactive oxygen species and β-catenin instability. Chin Med. 2016; 11:45. PMC: 5045596. DOI: 10.1186/s13020-016-0116-7. View

10.

Messeha S, Zarmouh N, Asiri A, Soliman K . Rosmarinic acid-induced apoptosis and cell cycle arrest in triple-negative breast cancer cells. Eur J Pharmacol. 2020; 885:173419. PMC: 7541730. DOI: 10.1016/j.ejphar.2020.173419. View

11.

Fang D, Xie H, Hu T, Shan H, Li M . Binding Features and Functions of ATG3. Front Cell Dev Biol. 2021; 9:685625. PMC: 8255673. DOI: 10.3389/fcell.2021.685625. View

12.

Sin Singer Brugiolo A, Carvalho Gouveia A, Alves C, de Castro E Silva F, de Oliveira E, Ferreira A . Ferulic acid supresses Th2 immune response and prevents remodeling in ovalbumin-induced pulmonary allergy associated with inhibition of epithelial-derived cytokines. Pulm Pharmacol Ther. 2017; 45:202-209. DOI: 10.1016/j.pupt.2017.07.001. View

13.

Zaharieva A, Rusanov K, Rusanova M, Paunov M, Yordanova Z, Mantovska D . Uncovering the Interrelation between Metabolite Profiles and Bioactivity of In Vitro- and Wild-Grown Catmint ( L.). Metabolites. 2023; 13(10). PMC: 10609352. DOI: 10.3390/metabo13101099. View

14.

Jang Y, Hwang K, Choi K . Rosmarinic Acid, a Component of Rosemary Tea, Induced the Cell Cycle Arrest and Apoptosis through Modulation of HDAC2 Expression in Prostate Cancer Cell Lines. Nutrients. 2018; 10(11). PMC: 6266655. DOI: 10.3390/nu10111784. View

15.

Amirzadeh M, Soltanian S, Mohamadi N . Chemical composition, anticancer and antibacterial activity of Nepeta mahanensis essential oil. BMC Complement Med Ther. 2022; 22(1):173. PMC: 9233784. DOI: 10.1186/s12906-022-03642-w. View

16.

Hu F, Li G, Huang C, Hou Z, Yang X, Luo X . The autophagy-independent role of BECN1 in colorectal cancer metastasis through regulating STAT3 signaling pathway activation. Cell Death Dis. 2020; 11(5):304. PMC: 7195408. DOI: 10.1038/s41419-020-2467-3. View

17.

Nouri A, Ghatreh-Samani K, Amini-Khoei H, Mohammadi A, Heidarian E, Najafi M . Ferulic acid prevents cyclosporine-induced nephrotoxicity in rats through exerting anti-oxidant and anti-inflammatory effects via activation of Nrf2/HO-1 signaling and suppression of NF-κB/TNF-α axis. Naunyn Schmiedebergs Arch Pharmacol. 2022; 395(4):387-395. DOI: 10.1007/s00210-022-02212-8. View

18.

Formisano C, Rigano D, Senatore F . Chemical constituents and biological activities of Nepeta species. Chem Biodivers. 2011; 8(10):1783-818. DOI: 10.1002/cbdv.201000191. View

19.

Bray F, Laversanne M, Sung H, Ferlay J, Siegel R, Soerjomataram I . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024; 74(3):229-263. DOI: 10.3322/caac.21834. View

20.

Liu Y, Shi L, Qiu W, Shi Y . Ferulic acid exhibits anti-inflammatory effects by inducing autophagy and blocking NLRP3 inflammasome activation. Mol Cell Toxicol. 2022; 18(4):509-519. PMC: 8744019. DOI: 10.1007/s13273-021-00219-5. View