Investigating the Cytotoxic Effects of Extract on Oral Carcinoma Cell Line

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2025 Jan 8

PMID 39767582

Authors

Ioannis Tsamesidis

Aliki Papadimitriou-Tsantarliotou

Athanasios Christodoulou

Dionysia Amanatidou

Chrysostomos Avgeros

Evangelia Stalika

Maria Bousnaki

Georgia Michailidou

Anastasia Beketova

Phaedra Eleftheriou

Dimitrios N Bikiaris

Ioannis S Vizirianakis

Eleana Kontonasaki

Affiliations

Soon will be listed here.

Abstract

, commonly known as absinthe, is a perennial plant with distinctive broad ovate pointed leaves of a silvery-gray color, reaching a height of 1.5 m. The utilization of this herb as a source of natural compounds and as the primary ingredient in the alcoholic beverage absinthe has recently seen a resurgence following a period of prohibition. This study investigates the biological effects of extract on healthy human periodontal ligament stem cells (hPDLSCs) and the human tongue squamous carcinoma cell line (HSC-3). element characterization was performed using High-Performance Liquid Chromatography (HPLC) and the Folin method. Alizarin assays evaluated the osteogenic capacity of human periodontal ligament cells (hPDLSCs) while CCK-8 and MTT determined the cytotoxicity of the extract against HSC-3 and hPDLSCs. High artemisinin levels were detected, revealing a concentration of 89 μM (25 μg/mL). The total phenolic concentration of the extract was 1.07 mM +/- 0.11. The in vitro cytotoxicity assays revealed the biocompatible profile of the extract in hPDLSCs without exhibiting any osteogenic potential. After 24 h of incubation with HSC-3, extract (10 µM) decreased cancer cell viability by 99% and artemisinin by 64%, and increased the expression of Caspase 3 and 9 almost six and two times, respectively. In summary, our preliminary findings suggest that extract exhibits a toxic effect against carcinoma cell lines without affecting healthy human periodontal ligament stem cells.

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