Method for Long-term Room Temperature Storage of Mouse Freeze-dried Sperm

Overview

Journal Sci Rep

Specialty Science

Date 2025 Jan 2

PMID 39747170

Authors

Yuko Kamada

Kango Yamaji

Natsuki Ushigome

Daiyu Ito

Sayaka Wakayama

Kenichiro Hiraoka

Masaru Hayashi

Kiyotaka Kawai

Teruhiko Wakayama

Affiliations

Soon will be listed here.

Abstract

Permanent preservation of genetic resources may be indispensable for the future of humanity. This requires liquid nitrogen, as is the case for preserving animal sperm. However, this technique is expensive and poses a risk of irrecoverable sample loss on non-replenishment of liquid nitrogen in case of natural disasters. In this study, we demonstrate that lyophilization may be used as a reliable method for long-term preservation of mouse sperm at room temperature. Sperm from four mouse strains were freeze-dried and stored in a non-temperature controlled room for 5-6 years. Although the ability of the stored sperm to activate oocytes had diminished slightly, healthy offspring were obtained by artificially activating the oocytes after sperm injection. Moreover, the birth rate did not decrease even after ≤ 6 years of storage. Furthermore, owing to its low cost, safety, and ease of storage at any location, we believe that this method could be a major mode of preserving mammalian genetic resources in the future.

Citing Articles

Can Humanity Thrive Beyond the Galaxy?.

Wakayama S, Wakayama T J Reprod Dev. 2025; 71(1):10-16.

PMID: 39756865 PMC: 11808306. DOI: 10.1262/jrd.2024-099.

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