Gene Expression Profiling in PBMCs for Acute Rejection in Lung Transplant Recipients Reveals Myeloid Responses

Overview

Journal Front Transplant

Specialty General Surgery

Date 2025 Jan 2

PMID 39744543

Authors

Siqi Liu

Johanna Westra

Shixian Hu

Erik A M Verschuuren

Leon C van Kempen

Debbie van Baarle

Nico A Bos

Affiliations

Soon will be listed here.

Abstract

The acute rejection (AR) diagnosis depends on transbronchial biopsy, which is semi-invasive and not easily performed Our study used the Nanostring gene expression technology on PBMCs obtained from lung transplant recipients (LTRs) to search for biomarkers. We identified distinct differential gene profiles between patients with stable status (STA) and AR. Subsequently, we independently evaluated monocyte compositions in PBMCs using flow cytometry and assessed the levels of 7 chemokines in serum using Luminex. The 48 top differentially expressed genes (DEGs) were identified, utilizing a criterion of at least a 1.5-fold change between two groups, with a false discovery rate (FDR) -Adj < 0.05. Of these 48 genes, the top 10 genes with the highest fold changes and significant -values were selected for qPCR validation. CD68, ANXA1, ITGB, and IFI30 can be confirmed among the validated genes. A significantly lower percentage of CD14 + CD16- classical monocytes was observed in AR than in STA patients, which aligns with downregulated DEGs. Many of the DEGs were related to monocytes-macrophages and chemokines. Although these results still need to be confirmed in larger cohorts, they suggest that gene profiling of PBMC can help to identify markers related to AR in LTRs.

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