Deciphering Pathogenicity and Virulence of the First Isolate from Diabetic Foot Osteomyelitis

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2025 Jan 1

PMID 39742337

Authors

Cynthia N Abi Najem

Chloe Magnan

Lucile Plumet

Nour Ahmad-Mansour

Cassandra Pouget

Madjid Morsli

Alix Pantel

Karima Kissa

Albert Sotto

Jean-Philippe Lavigne

Virginie Molle

Affiliations

Soon will be listed here.

Abstract

Introduction: This study identifies as a new coagulase-negative staphylococcal species isolated from diabetic foot osteomyelitis (DFOM) and provides an in-depth analysis of its pathogenic and virulence profile, as well as demonstrating its potential to cause infection.

Methods: The NSD001 strain was examined for its planktonic growth, biofilm production, and phagocytosis rates in murine macrophages compared to NSA739. Additionally, persistence and replication within human osteoblasts were investigated, while the zebrafish embryo model was employed to assess virulence. Genomic sequencing and bioinformatic analysis were also conducted to identify genes associated with virulent potential.

Results And Discussion: NSD001 exhibited robust planktonic growth and significant biofilm production, highlighting its capacity to initiate and maintain an infection, and demonstrated similar rates of phagocytosis as NSA739 in murine macrophages, suggesting a mechanism for evading initial host defenses. The strain persisted and replicated within human osteoblasts, indicative of a strategy for intracellular survival and facilitation of chronic osteomyelitis. The zebrafish embryo model revealed a slower, yet fatal, virulence profile for NSD001 compared to the rapid lethality induced by NSA739. Genomic sequencing and bioinformatic analysis uncovered various genes corroborating its virulence. NSD001 poses a significant concern in DFOM due to its ability to form biofilms and survive within host cells, presenting challenges for current treatment strategies. This underscores the need for updated clinical protocols and increased awareness among healthcare professionals to effectively manage infections caused by this emerging pathogen.

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