Pathological, Hematological, and Biochemical Alteration in Broiler Chickens Infected with Mycotoxin in Babylon Province

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2024 Dec 31

PMID 39737046

Authors

Hayder Abd Al-Emier Almremdhy

Ghusoon Abdul Kareem Neamha

Zina Bakir Al-Hilli

Rafal Jalil Awadh

Affiliations

Soon will be listed here.

Abstract

Background: Mycotoxins are considered one of the most important problems and threats that face poultry producers.

Aim: This study was conducted to investigate the pathological, hematological, and biochemical alterations in chickens fed on mycotoxins contamination ration.

Methods: 434 feed samples were collected from poultry farms operating in Babil Governorate/Iraq, where feed samples were collected over the course of 2023, and these samples were tested by direct competitive enzyme-linked immunosorbent assay to determine the level of mycotoxins. (Aflatoxin, Ochratoxin, and Trichothecin T2) in poultry feed rations, after that, the chickens that were fed on feeds contaminated with mycotoxins were carefully examined to observe clinical signs. Then, blood samples were collected from chickens fed on feeds contaminated with mycotoxins, as well as from chickens fed on uncontaminated feeds (control group). These blood samples were divided to two parts one was put in a tube contain anticoagulant Ethylenediaminetetraacetic acid (EDTA) to examination the complete blood count, while the other part was put in a gel test tube for separating the serum which used in the biochemical tests which included the total protein and total cholesterol, uric acid and the liver enzymes [Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST)]. Then, the chickens fed on mycotoxins contaminated feed were humanly sacrificed to observe the gross lesions after that, tissue samples were collected from internal organs (liver, kidneys, bursa of Fabricius, and spleen) to examine the microscopic lesions.

Results: The results showed the percentage of feed samples contaminated with mycotoxins above the limit of quantification values were 22% (94), whereas the percentage of feed samples contaminated with aflatoxin, ochratoxin, and trechothesen T2 was 12% (54), 8% (34), and 2% (6), respectively. The gross lesions were showed on the internal organs of chickens fed on mycotoxins contaminated feed paleness, enlargement and friable liver, pale, enlarged, and lobulated kidneys, hemorrhage in skeletal muscle as well as showed ascites. Microscopically, the kidneys showed necrosis in some renal tubules and glomeruli. In the liver, there was congestion of the portal vein and periportal necrosis with inflammatory cells infiltration. The results showed a significant decrease in means values of HB, PCV, RBCS, WBCS, total protein, and cholesterol while there was a significant increase in means values of AST, ALT, and Uric acid in infected chickens group compared with the healthy chicken group.

Conclusion: Contamination of feed with mycotoxins is one of the most prominent challenges facing poultry producers in Babylon province. In addition, it is important to do pathological, hematological, and biochemical examinations for the diagnosis of mycotoxins in broilers.

References

Streit E, Schatzmayr G, Tassis P, Tzika E, Marin D, Taranu I . Current situation of mycotoxin contamination and co-occurrence in animal feed--focus on Europe. Toxins (Basel). 2012; 4(10):788-809. PMC: 3496989. DOI: 10.3390/toxins4100788. View

Wang A, Hogan N . Performance effects of feed-borne mycotoxins on broiler chickens: Influences of timing and duration of exposure. Anim Nutr. 2019; 5(1):32-40. PMC: 6407085. DOI: 10.1016/j.aninu.2018.08.001. View

Antonissen G, Van Immerseel F, Pasmans F, Ducatelle R, Haesebrouck F, Timbermont L . The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. PLoS One. 2014; 9(9):e108775. PMC: 4182565. DOI: 10.1371/journal.pone.0108775. View

Raju M, Devegowda G . Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). Br Poult Sci. 2001; 41(5):640-50. DOI: 10.1080/713654986. View

Ortatatli M, Oguz H, Hatipoglu F, Karaman M . Evaluation of pathological changes in broilers during chronic aflatoxin (50 and 100 ppb) and clinoptilolite exposure. Res Vet Sci. 2004; 78(1):61-8. DOI: 10.1016/j.rvsc.2004.06.006. View

Williams J, Phillips T, Jolly P, Stiles J, Jolly C, Aggarwal D . Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions. Am J Clin Nutr. 2004; 80(5):1106-22. DOI: 10.1093/ajcn/80.5.1106. View

Gowda N, Ledoux D, Rottinghaus G, Bermudez A, Chen Y . Efficacy of turmeric (Curcuma longa), containing a known level of curcumin, and a hydrated sodium calcium aluminosilicate to ameliorate the adverse effects of aflatoxin in broiler chicks. Poult Sci. 2008; 87(6):1125-30. DOI: 10.3382/ps.2007-00313. View

Kubena L, Harvey R, Phillips T, Clement B . Effect of hydrated sodium calcium aluminosilicates on aflatoxicosis in broiler chicks. Poult Sci. 1993; 72(4):651-7. DOI: 10.3382/ps.0720651. View

Perrone G, Susca A, Cozzi G, Ehrlich K, Varga J, Frisvad J . Biodiversity of Aspergillus species in some important agricultural products. Stud Mycol. 2008; 59:53-66. PMC: 2275197. DOI: 10.3114/sim.2007.59.07. View

10.

Hashem M, Mohamed M . Haemato-biochemical and pathological studies on aflatoxicosis and treatment of broiler chicks in Egypt. Vet Ital. 2010; 45(2):323-37. View

11.

Abd El-Hack M, Samak D, Noreldin A, El-Naggar K, Abdo M . Probiotics and plant-derived compounds as eco-friendly agents to inhibit microbial toxins in poultry feed: a comprehensive review. Environ Sci Pollut Res Int. 2018; 25(32):31971-31986. DOI: 10.1007/s11356-018-3197-2. View

12.

Gholami-Ahangaran M, Rangsaz N, Azizi S . Evaluation of turmeric (Curcuma longa) effect on biochemical and pathological parameters of liver and kidney in chicken aflatoxicosis. Pharm Biol. 2015; 54(5):780-7. DOI: 10.3109/13880209.2015.1080731. View

13.

Pappas A, Tsiplakou E, Tsitsigiannis D, Georgiadou M, Iliadi M, Sotirakoglou K . The role of bentonite binders in single or concomitant mycotoxin contamination of chicken diets. Br Poult Sci. 2016; 57(4):551-8. DOI: 10.1080/00071668.2016.1187712. View

14.

Kumar A, Jindal N, Shukla C, Asrani R, Ledoux D, Rottinghaus G . Pathological changes in broiler chickens fed ochratoxin A and inoculated with Escherichia coli. Avian Pathol. 2004; 33(4):413-7. DOI: 10.1080/03079450410001724021. View

15.

Magnoli A, Monge M, Miazzo R, Cavaglieri L, Magnoli C, Merkis C . Effect of low levels of aflatoxin B₁ on performance, biochemical parameters, and aflatoxin B₁ in broiler liver tissues in the presence of monensin and sodium bentonite. Poult Sci. 2010; 90(1):48-58. DOI: 10.3382/ps.2010-00971. View

16.

Dutta T, Das P . Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxin B1 from feeds in India. Mycopathologia. 2001; 151(1):29-33. DOI: 10.1023/a:1010960402254. View

17.

Zhao J, Shirley R, Dibner J, Uraizee F, Officer M, Kitchell M . Comparison of hydrated sodium calcium aluminosilicate and yeast cell wall on counteracting aflatoxicosis in broiler chicks. Poult Sci. 2010; 89(10):2147-56. DOI: 10.3382/ps.2009-00608. View

18.

Ma R, Zhang L, Liu M, Su Y, Xie W, Zhang N . Individual and Combined Occurrence of Mycotoxins in Feed Ingredients and Complete Feeds in China. Toxins (Basel). 2018; 10(3). PMC: 5869401. DOI: 10.3390/toxins10030113. View

19.

Olalekan Adeyeye S . Aflatoxigenic fungi and mycotoxins in food: a review. Crit Rev Food Sci Nutr. 2019; 60(5):709-721. DOI: 10.1080/10408398.2018.1548429. View