Occurrence of Taenia Species and Toxoplasma Gondii in Pigs Slaughtered in Bujumbura City, Kayanza and Ngozi Provinces, Burundi

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2024 Dec 30

PMID 39736692

Authors

Salvator Minani

Emma Spiessens

Alyssa Labarriere

Pascal Niyokwizera

Anastasie Gasogo

Jean-Bosco Ntirandekura

Chiara Trevisan

Sarah Gabriel

Affiliations

Soon will be listed here.

Abstract

Background: Taenia spp. and Toxoplasma gondii are foodborne parasites affecting humans and pigs. The magnitude of the burden of these parasites in pigs in Burundi is not known. Therefore, this study aimed to estimate the prevalence of Taenia spp. infections in pigs by meat inspection, partial carcass dissection and molecular confirmation and estimate the prevalence of Toxoplasma gondii infection in pigs by serology. A cross-sectional study was conducted in pig slaughter slabs located in Bujumbura city, Kayanza and Ngozi provinces. Multisampling strategies were used to sample 576 pigs. Upon pig slaughter, blood samples were collected to perform indirect ELISA for detecting antibodies against the T. gondii P30 protein in the serum. Routine meat inspection was carried out to detect T. solium and T. hydatigena cysticerci. The tongue, heart and masseter muscles were dissected by making slices less than 5 mm thick to estimate the intensity and stages of T. solium cysticerci. A subset of cysticerci and suspected lesions per infected pig were examined using PCR-RFLP to differentiate Taenia spp.

Results: Of the 576 pigs, 14 (2.4%) were positive for T. solium cysticercosis by meat inspection and 67 (11.6%) by partial carcass dissection. After molecular analysis, 66 (11.5%) samples were confirmed to be T. solium infections. The average of T. solium cysticerci in the dissected organs was estimated at 80 cysticerci. Most cysticerci (76.1%) were counted in the masseter muscles, followed by the tongue (18.8%) and the heart (5.1%). The majority of cysticerci (88.3%) were viable, 6.4% were calcified and 5.3% were degenerated. Approximately 69% of the pigs infected with T. solium had light infections, 13.4% had moderate infections and 17.9% had heavy infections. Thirty-two out of 576 pigs (5.5%) were suspected of being infected with T. hydatigena by meat inspection, but 24 pigs (4.2%) were confirmed molecularly to be positive for T. hydatigena infection. The seroprevalence of T. gondii infection in pigs was 17.7%.

Conclusions: This study indicates that T. solium and T. gondii parasites are endemic in Burundi and provides evidence of potential public health risks for the local population. Effective control strategies, including improved pig farming practices, better hygiene and sanitation, increased meat inspection, monitoring of infected pigs, risk-free culinary practices, and treatment of tapeworm carriers, should be implemented to avoid the perpetual contamination of pigs and humans with these zoonotic parasites.

References

Sarti E, Rajshekhar V . Measures for the prevention and control of Taenia solium taeniosis and cysticercosis. Acta Trop. 2003; 87(1):137-43. DOI: 10.1016/s0001-706x(03)00034-2. View

Excler J, Pretat E, Pozzetto B, Charpin B, GARIN J . Sero-epidemiological survey for toxoplasmosis in Burundi. Trop Med Parasitol. 1988; 39(2):139-41. View

Carabin H, Ndimubanzi P, Budke C, Nguyen H, Qian Y, Cowan L . Clinical manifestations associated with neurocysticercosis: a systematic review. PLoS Negl Trop Dis. 2011; 5(5):e1152. PMC: 3101170. DOI: 10.1371/journal.pntd.0001152. View

Lopez-Urena N, Calero-Bernal R, Vazquez-Calvo A, Sanchez-Sanchez R, Ortega-Mora L, Alvarez-Garcia G . A comparative study of serological tests used in the diagnosis of Toxoplasma gondii infection in small ruminants evidenced the importance of cross-reactions for harmonizing diagnostic performance. Res Vet Sci. 2023; 165:105052. DOI: 10.1016/j.rvsc.2023.105052. View

Phiri I, Dorny P, Gabriel S, Willingham 3rd A, Sikasunge C, Siziya S . Assessment of routine inspection methods for porcine cysticercosis in Zambian village pigs. J Helminthol. 2006; 80(1):69-72. DOI: 10.1079/joh2005314. View

Sithole M, Bekker J, Tsotetsi-Khambule A, Mukaratirwa S . Ineffectiveness of meat inspection in the detection of Taenia solium cysticerci in pigs slaughtered at two abattoirs in the Eastern Cape Province of South Africa. Vet Parasitol Reg Stud Reports. 2019; 17:100299. DOI: 10.1016/j.vprsr.2019.100299. View

Smith N, Goulart C, Hayward J, Kupz A, Miller C, van Dooren G . Control of human toxoplasmosis. Int J Parasitol. 2020; 51(2-3):95-121. DOI: 10.1016/j.ijpara.2020.11.001. View

Ndimubanzi P, Carabin H, Budke C, Nguyen H, Qian Y, Rainwater E . A systematic review of the frequency of neurocyticercosis with a focus on people with epilepsy. PLoS Negl Trop Dis. 2010; 4(11):e870. PMC: 2970544. DOI: 10.1371/journal.pntd.0000870. View

Molan A, Nosaka K, Hunter M, Wang W . Global status of Toxoplasma gondii infection: systematic review and prevalence snapshots. Trop Biomed. 2021; 36(4):898-925. View

10.

Lightowlers M, Assana E, Jayashi C, Gauci C, Donadeu M . Sensitivity of partial carcass dissection for assessment of porcine cysticercosis at necropsy. Int J Parasitol. 2015; 45(13):815-8. PMC: 4655835. DOI: 10.1016/j.ijpara.2015.08.004. View

11.

Dubey J . Toxoplasmosis in pigs--the last 20 years. Vet Parasitol. 2009; 164(2-4):89-103. DOI: 10.1016/j.vetpar.2009.05.018. View

12.

Dorny P, Phiri I, Vercruysse J, Gabriel S, Willingham 3rd A, Brandt J . A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. Int J Parasitol. 2004; 34(5):569-76. DOI: 10.1016/j.ijpara.2003.11.014. View

13.

Assana E, Awah-Ndukum J, Djonmaila J, Zoli A . Prevalence of porcine Taenia solium and Taenia hydatigena cysticercosis in Cameroon. Prev Vet Med. 2019; 169:104690. DOI: 10.1016/j.prevetmed.2019.104690. View

14.

Mkupasi E, Ngowi H, Sikasunge C, Leifsson P, Johansen M . Distribution and histopathological changes induced by cysts of Taenia solium in the brain of pigs from Tanzania. J Helminthol. 2014; 89(5):559-64. DOI: 10.1017/S0022149X14000418. View

15.

Nguyen M, Gabriel S, Abatih E, Dorny P . A systematic review on the global occurrence of Taenia hydatigena in pigs and cattle. Vet Parasitol. 2016; 226:97-103. DOI: 10.1016/j.vetpar.2016.06.034. View

16.

Dubey J, Cerqueira-Cezar C, Murata F, Kwok O, Hill D, Yang Y . All about Toxoplasma gondii infections in pigs: 2009-2020. Vet Parasitol. 2020; 288:109185. DOI: 10.1016/j.vetpar.2020.109185. View

17.

Kabululu M, Johansen M, Lightowlers M, Trevisan C, Braae U, Ngowi H . Aggregation of cysticerci in pigs: Implications for transmission and control. Parasite Epidemiol Control. 2023; 22:e00307. PMC: 10227459. DOI: 10.1016/j.parepi.2023.e00307. View

18.

Coral-Almeida M, Gabriel S, Abatih E, Praet N, Benitez W, Dorny P . Taenia solium Human Cysticercosis: A Systematic Review of Sero-epidemiological Data from Endemic Zones around the World. PLoS Negl Trop Dis. 2015; 9(7):e0003919. PMC: 4493064. DOI: 10.1371/journal.pntd.0003919. View

19.

Singh S, Singh B, Kalambhe D, Pathak D, Aulakh R, Dhand N . Prevalence and distribution of Taenia solium cysticercosis in naturally infected pigs in Punjab, India. PLoS Negl Trop Dis. 2018; 12(11):e0006960. PMC: 6264866. DOI: 10.1371/journal.pntd.0006960. View

20.

Dubey J, Kotula A, Sharar A, Andrews C, Lindsay D . Effect of high temperature on infectivity of Toxoplasma gondii tissue cysts in pork. J Parasitol. 1990; 76(2):201-4. View