Enhancing Bone Regeneration Through 3D Printed Biphasic Calcium Phosphate Scaffolds Featuring Graded Pore Sizes

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2024 Dec 30

PMID 39734570

Authors

Yue Wang

Yang Liu

Shangsi Chen

Ming-Fung Francis Siu

Chao Liu

Jiaming Bai

Min Wang

Affiliations

Soon will be listed here.

Abstract

Human long bones exhibit pore size gradients with small pores in the exterior cortical bone and large pores in the interior cancellous bone. However, most current bone tissue engineering (BTE) scaffolds only have homogeneous porous structures that do not resemble the graded architectures of natural bones. Pore-size graded (PSG) scaffolds are attractive for BTE since they can provide biomimicking porous structures that may lead to enhanced bone tissue regeneration. In this study, uniform pore size scaffolds and PSG scaffolds were designed using the gyroid unit of triply periodic minimal surface (TPMS), with small pores (400 μm) in the periphery and large pores (400, 600, 800 or 1000 μm) in the center of BTE scaffolds (designated as 400-400, 400-600, 400-800, and 400-1000 scaffold, respectively). All scaffolds maintained the same porosity of 70 vol%. BTE scaffolds were subsequently fabricated through digital light processing (DLP) 3D printing with the use of biphasic calcium phosphate (BCP). The results showed that DLP 3D printing could produce PSG BCP scaffolds with high fidelity. The PSG BCP scaffolds possessed improved biocompatibility and mass transport properties as compared to uniform pore size BCP scaffolds. In particular, the 400-800 PSG scaffolds promoted osteogenesis and enhanced new bone formation and vascularization while they displayed favorable compressive properties and permeability. This study has revealed the importance of structural design and optimization of BTE scaffolds for achieving balanced mechanical, mass transport and biological performance for bone regeneration.

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References

Liu K, Zhou Q, Zhang X, Ma L, Xu B, He R . Morphologies, mechanical and behaviors of DLP-based 3D printed HA scaffolds with different structural configurations. RSC Adv. 2023; 13(30):20830-20838. PMC: 10333813. DOI: 10.1039/d3ra03080f. View

Daculsi G, Laboux O, Malard O, Weiss P . Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med. 2004; 14(3):195-200. DOI: 10.1023/a:1022842404495. View

Carter D, Spengler D . Mechanical properties and composition of cortical bone. Clin Orthop Relat Res. 1978; (135):192-217. View

Montazerian H, Mohamed M, Montazeri M, Kheiri S, Milani A, Kim K . Permeability and mechanical properties of gradient porous PDMS scaffolds fabricated by 3D-printed sacrificial templates designed with minimal surfaces. Acta Biomater. 2019; 96:149-160. DOI: 10.1016/j.actbio.2019.06.040. View

Zhang L, Wang B, Song B, Yao Y, Choi S, Yang C . 3D Printed Biomimetic Metamaterials with Graded Porosity and Tapering Topology for Improved Cell Seeding and Bone Regeneration. Bioact Mater. 2023; 25:677-688. PMC: 10087492. DOI: 10.1016/j.bioactmat.2022.07.009. View

Bouler J, Pilet P, Gauthier O, Verron E . Biphasic calcium phosphate ceramics for bone reconstruction: A review of biological response. Acta Biomater. 2017; 53:1-12. DOI: 10.1016/j.actbio.2017.01.076. View

Giannitelli S, Accoto D, Trombetta M, Rainer A . Current trends in the design of scaffolds for computer-aided tissue engineering. Acta Biomater. 2013; 10(2):580-94. DOI: 10.1016/j.actbio.2013.10.024. View

Bobbert F, Zadpoor A . Effects of bone substitute architecture and surface properties on cell response, angiogenesis, and structure of new bone. J Mater Chem B. 2020; 5(31):6175-6192. DOI: 10.1039/c7tb00741h. View

Pan J, Wu L, Cai J, Wu L, Liang M . Dexamethasone suppresses osteogenesis of osteoblast the PI3K/Akt signaling pathway and . J Recept Signal Transduct Res. 2019; 39(1):80-86. DOI: 10.1080/10799893.2019.1625061. View

10.

Bonjour J . Calcium and phosphate: a duet of ions playing for bone health. J Am Coll Nutr. 2011; 30(5 Suppl 1):438S-48S. DOI: 10.1080/07315724.2011.10719988. View

11.

Xia D, Qin Y, Guo H, Wen P, Lin H, Voshage M . Additively manufactured pure zinc porous scaffolds for critical-sized bone defects of rabbit femur. Bioact Mater. 2022; 19:12-23. PMC: 8980439. DOI: 10.1016/j.bioactmat.2022.03.010. View

12.

Pranav Adithya S, Saleth Sidharthan D, Abhinandan R, Balagangadharan K, Selvamurugan N . Nanosheets-incorporated bio-composites containing natural and synthetic polymers/ceramics for bone tissue engineering. Int J Biol Macromol. 2020; 164:1960-1972. DOI: 10.1016/j.ijbiomac.2020.08.053. View

13.

Sola A, Bellucci D, Cannillo V . Functionally graded materials for orthopedic applications - an update on design and manufacturing. Biotechnol Adv. 2016; 34(5):504-531. DOI: 10.1016/j.biotechadv.2015.12.013. View

14.

Ma S, Tang Q, Feng Q, Song J, Han X, Guo F . Mechanical behaviours and mass transport properties of bone-mimicking scaffolds consisted of gyroid structures manufactured using selective laser melting. J Mech Behav Biomed Mater. 2019; 93:158-169. DOI: 10.1016/j.jmbbm.2019.01.023. View

15.

Altunbek M, Afghah S, Fallah A, Acar A, Koc B . Design and 3D Printing of Personalized Hybrid and Gradient Structures for Critical Size Bone Defects. ACS Appl Bio Mater. 2023; 6(5):1873-1885. PMC: 10189796. DOI: 10.1021/acsabm.3c00107. View

16.

Visscher L, Dang H, Knackstedt M, Hutmacher D, Tran P . 3D printed Polycaprolactone scaffolds with dual macro-microporosity for applications in local delivery of antibiotics. Mater Sci Eng C Mater Biol Appl. 2018; 87:78-89. DOI: 10.1016/j.msec.2018.02.008. View

17.

Temp R, Packaeser M, Machry R, Dapieve K, Rippe M, Pereira G . Characteristic fatigue strength and reliability of dental glass-ceramics: Effect of distinct surface treatments - Hydrofluoric acid etching and silane treatment vs one-step self-etching ceramic primer. J Mech Behav Biomed Mater. 2023; 150:106338. DOI: 10.1016/j.jmbbm.2023.106338. View

18.

Liu Q, Wei F, Coathup M, Shen W, Wu D . Effect of Porosity and Pore Shape on the Mechanical and Biological Properties of Additively Manufactured Bone Scaffolds. Adv Healthc Mater. 2023; 12(30):e2301111. DOI: 10.1002/adhm.202301111. View

19.

Pagani S, Liverani E, Giavaresi G, De Luca A, Belvedere C, Fortunato A . Mechanical and in vitro biological properties of uniform and graded Cobalt-chrome lattice structures in orthopedic implants. J Biomed Mater Res B Appl Biomater. 2021; 109(12):2091-2103. PMC: 8518749. DOI: 10.1002/jbm.b.34857. View

20.

Chaudhary L, Hofmeister A, Hruska K . Differential growth factor control of bone formation through osteoprogenitor differentiation. Bone. 2004; 34(3):402-11. DOI: 10.1016/j.bone.2003.11.014. View