Optimization of Fermentation Conditions for Whole Cell Catalytic Synthesis of D-allulose by Engineering Escherichia Coli

Overview

Journal Sci Rep

Specialty Science

Date 2024 Dec 27

PMID 39730529

Authors

Haoran Liu

Kang Xu

Shuqi Sun

Yinbiao Wan

Bojia Zhang

Yang Song

Chuanzhuang Guo

Songsen Sui

Ruiming Wang

Piwu Li

Junqing Wang

Zhenshang Xu

Ting Wang

Affiliations

Soon will be listed here.

Abstract

D-allulose/D-psicose is a significant rare sugar with broad applications in the pharmaceutical, food, and other industries. In this study, we cloned the D-allulose 3-epimerase (DPEase) gene from Arthrobacter globiformis M30, using pET22b as the vector. The recombinant E. coli strain pET22b(+) was successfully constructed and expressed, providing an efficient whole-cell catalyst for converting inexpensive D-fructose into D-allulose. Subsequently, we optimized the induction and incubation conditions step by step using the single-factor method and used Lactobacillus plantarum(LAB) 217-8 to enhance the purity of D-allulose in the system. Ultimately, the BL21/pET22b(+)-E. coli strain achieved a conversion rate of up to 33.91% under optimal conditions, converting D-fructose to D-allulose. After purification, the purity of D-allulose reached 64.73%. Efficient production of D-allulose is a significant achievement, paving the way for future probiotic applications in its conversion.

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