Biological and Chemical Management of and Ochratoxin A in Vineyards

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2024 Dec 27

PMID 39728785

Authors

Maria K Iliadi

Maria Varveri

Dimitrios I Tsitsigiannis

Affiliations

Soon will be listed here.

Abstract

Ochratoxin A (OTA) is a widely distributed mycotoxin and potent carcinogen produced by several fungal genera, but mainly by . Grape contamination occurs in vineyards during the period between veraison and pre-harvest, and it is the main cause of OTA's presence in wine. The aim of the current study was the evaluation of 6 chemical and 11 biological plant protection products (PPPs) and biocontrol agents in commercial vineyards of the two important Greek white wine varieties cv. Malagousia and cv. Savatiano. The PPPs were applied in a 4-year vineyard study as single treatments or/and in combinations as part of IPM systems. Subsequently, nine strains of were investigated for their sensitivity against seven active compounds of synthetic fungicides. During the multi-year field trials, various novel management systems, including consortia of biocontrol agents, were revealed to be effective against Aspergillus sour rot and OTA production. However, expected variability was observed in the experimental results, indicating the dynamic character of biological systems and highlighting the possible inconsistency of PPPs' efficacy in a changing environment. Furthermore, the IPM systems developed effectuated an optimized control of , leading to 100% inhibition of OTA contamination, showing the importance of using both chemical and biological PPPs for disease management and prevention of fungal fungicide resistance. Finally, the majority of tested strains were found to be sensitive against the pure active compounds used (fludioxonil, azoxystrobin, chlorothalonil, tebuconazole, cyprodinil, pyrimethanil and boscalid), with only a few exceptions of developed resistance towards boscalid.

References

Wang G, Li E, Gallo A, Perrone G, Varga E, Ma J . Impact of environmental factors on ochratoxin A: From natural occurrence to control strategy. Environ Pollut. 2022; 317:120767. DOI: 10.1016/j.envpol.2022.120767. View

Chang X, Wu Z, Wu S, Dai Y, Sun C . Degradation of ochratoxin A by Bacillus amyloliquefaciens ASAG1. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2014; 32(4):564-71. DOI: 10.1080/19440049.2014.991948. View

Venkatesh N, Keller N . Mycotoxins in Conversation With Bacteria and Fungi. Front Microbiol. 2019; 10:403. PMC: 6433837. DOI: 10.3389/fmicb.2019.00403. View

Alshannaq A, Yu J . Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food. Int J Environ Res Public Health. 2017; 14(6). PMC: 5486318. DOI: 10.3390/ijerph14060632. View

Khoury R, Atoui A, Verheecke C, Maroun R, El Khoury A, Mathieu F . Essential Oils Modulate Gene Expression and Ochratoxin A Production in Aspergillus carbonarius. Toxins (Basel). 2016; 8(8). PMC: 4999858. DOI: 10.3390/toxins8080242. View

Passamani F, Hernandes T, Lopes N, Bastos S, Santiago W, Cardoso M . Effect of temperature, water activity, and pH on growth and production of ochratoxin A by Aspergillus niger and Aspergillus carbonarius from Brazilian grapes. J Food Prot. 2014; 77(11):1947-52. DOI: 10.4315/0362-028X.JFP-13-495. View

Kontaxakis E, Fysarakis I, Lydakis D, Magan N . Farming System Effect on the Incidence of Aspergillus carbonarius on Kotsifali Grapes and Ochratoxin A Occurrence in Wines of Crete. J Food Prot. 2020; 83(10):1796-1800. DOI: 10.4315/JFP-20-133. View

Masiello M, Somma S, Haidukowski M, Logrieco A, Moretti A . Genetic polymorphisms associated to SDHI fungicides resistance in selected Aspergillus flavus strains and relation with aflatoxin production. Int J Food Microbiol. 2020; 334:108799. DOI: 10.1016/j.ijfoodmicro.2020.108799. View

BELLI N, Marin S, Sanchis V, Ramos A . Impact of fungicides on Aspergillus carbonarius growth and ochratoxin A production on synthetic grape-like medium and on grapes. Food Addit Contam. 2006; 23(10):1021-9. DOI: 10.1080/02652030600778702. View

10.

Ben Miri Y, Benabdallah A, Chentir I, Djenane D, Luvisi A, De Bellis L . Comprehensive Insights into Ochratoxin A: Occurrence, Analysis, and Control Strategies. Foods. 2024; 13(8). PMC: 11049263. DOI: 10.3390/foods13081184. View

11.

De Curtis F, de Felice D, Ianiri G, De Cicco V, Castoria R . Environmental factors affect the activity of biocontrol agents against ochratoxigenic Aspergillus carbonarius on wine grape. Int J Food Microbiol. 2012; 159(1):17-24. DOI: 10.1016/j.ijfoodmicro.2012.07.023. View

12.

Visconti A, Perrone G, Cozzi G, Solfrizzo M . Managing ochratoxin A risk in the grape-wine food chain. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008; 25(2):193-202. DOI: 10.1080/02652030701744546. View

13.

Kalagatur N, Gurunathan S, Kamasani J, Gunti L, Kadirvelu K, Mohan C . Inhibitory effect of , , , , and essential oils on growth and ochratoxin A content of and in maize grains. Biotechnol Rep (Amst). 2020; 27:e00490. PMC: 7327888. DOI: 10.1016/j.btre.2020.e00490. View

14.

Leong S, Hocking A, Pitt J, Kazi B, Emmett R, Scott E . Australian research on ochratoxigenic fungi and ochratoxin A. Int J Food Microbiol. 2006; 111 Suppl 1:S10-7. DOI: 10.1016/j.ijfoodmicro.2006.02.005. View

15.

Hua H, Xing F, Selvaraj J, Wang Y, Zhao Y, Zhou L . Inhibitory effect of essential oils on Aspergillus ochraceus growth and ochratoxin A production. PLoS One. 2014; 9(9):e108285. PMC: 4178002. DOI: 10.1371/journal.pone.0108285. View

16.

Valero A, Marin S, Ramos A, Sanchis V . Effect of preharvest fungicides and interacting fungi on Aspergillus carbonarius growth and ochratoxin A synthesis in dehydrating grapes. Lett Appl Microbiol. 2007; 45(2):194-9. DOI: 10.1111/j.1472-765X.2007.02168.x. View

17.

Mwabulili F, Xie Y, Li Q, Sun S, Yang Y, Ma W . Research progress of ochratoxin a bio-detoxification. Toxicon. 2022; 222:107005. DOI: 10.1016/j.toxicon.2022.107005. View

18.

Kizis D, Natskoulis P, Nychas G, Panagou E . Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece. PLoS One. 2014; 9(4):e93923. PMC: 3977974. DOI: 10.1371/journal.pone.0093923. View

19.

Terra M, Lira N, Passamani F, Santiago W, Cardoso M, Batista L . Effect of Fungicides on Growth and Ochratoxin A Production by Aspergillus carbonarius from Brazilian Wine Grapes. J Food Prot. 2017; 79(9):1508-1516. DOI: 10.4315/0362-028X.JFP-16-037. View

20.

de Felice D, Solfrizzo M, De Curtis F, Lima G, Visconti A, Castoria R . Strains of Aureobasidium pullulans can lower ochratoxin A contamination in wine grapes. Phytopathology. 2008; 98(12):1261-70. DOI: 10.1094/PHYTO-98-12-1261. View