Association of Infection with Colorectal Cancer in Kazakhstani Patients

Overview

Journal Front Oncol

Specialty Oncology

Date 2024 Dec 27

PMID 39726700

Authors

Gulmira Kulmambetova

Botakoz Kurentay

Alua Gusmaulemova

Talgat Utupov

Dana Auganova

Pavel Tarlykov

Meiram Mamlin

Saule Khamzina

Sanzhar Shalekenov

Arman Kozhakhmetov

Affiliations

Soon will be listed here.

Abstract

Objectives: is a gram-negative anaerobic bacillus associated with colorectal cancer (CRC). We aimed to determine the abundance of . and other CRC-associated bacteria using quantitative real-time polymerase chain reaction (qPCR) analysis to detect the possible correlations between tumor and normal tissues and the relationships between patients' clinical characteristics, diet, and CRC-associated bacteria.

Methods: A total of 249 biopsy samples of tumor and paired normal tissues were collected from patients with CRC. Biopsy samples were screened for detection of using qPCR targeting gene. , , and were also detected in the samples using species-specific genes.

Results: The frequencies of detection of in the tumor and normal tissues of patients with CRC were 43.37 and 24.1%, respectively (P < 0.05). Statistical analysis using cycle threshold (Ct) values from qPCR data and clinical characteristics showed that tumor size, tumor location, and processed meat consumption were significantly correlated with the abundance of (P < 0.05). The significance of the prevalence of and in tumor tissues was marginally higher than that in normal tissues (P < 0.1), and the consumption of processed/red meat affected the prevalence of these bacteria (P < 0.05).

Conclusions: Our results showed an association between the presence of in tumor tissues and CRC, indicating that may be a potential marker for CRC diagnosis. is enriched in CRC tissues and is associated with CRC development.

References

Mima K, Sukawa Y, Nishihara R, Qian Z, Yamauchi M, Inamura K . Fusobacterium nucleatum and T Cells in Colorectal Carcinoma. JAMA Oncol. 2015; 1(5):653-61. PMC: 4537376. DOI: 10.1001/jamaoncol.2015.1377. View

Zhang S, Cai S, Ma Y . Association between and colorectal cancer: Progress and future directions. J Cancer. 2018; 9(9):1652-1659. PMC: 5950595. DOI: 10.7150/jca.24048. View

Li X, Huang J, Yu T, Fang X, Lou L, Xin S . Promotes the Progression of Colorectal Cancer Through Cdk5-Activated Wnt/β-Catenin Signaling. Front Microbiol. 2021; 11:545251. PMC: 7815597. DOI: 10.3389/fmicb.2020.545251. View

Yu J, Chen Y, Fu X, Zhou X, Peng Y, Shi L . Invasive Fusobacterium nucleatum may play a role in the carcinogenesis of proximal colon cancer through the serrated neoplasia pathway. Int J Cancer. 2016; 139(6):1318-26. DOI: 10.1002/ijc.30168. View

Yun M, You S, Nguyen V, Prakash J, Glasl S, Gujrati V . Reporter gene-based optoacoustic imaging of E. coli targeted colon cancer in vivo. Sci Rep. 2021; 11(1):24430. PMC: 8709855. DOI: 10.1038/s41598-021-04047-4. View

Wang N, Fang J . Fusobacterium nucleatum, a key pathogenic factor and microbial biomarker for colorectal cancer. Trends Microbiol. 2022; 31(2):159-172. DOI: 10.1016/j.tim.2022.08.010. View

Liu L, Tabung F, Zhang X, Nowak J, Qian Z, Hamada T . Diets That Promote Colon Inflammation Associate With Risk of Colorectal Carcinomas That Contain Fusobacterium nucleatum. Clin Gastroenterol Hepatol. 2018; 16(10):1622-1631.e3. PMC: 6151288. DOI: 10.1016/j.cgh.2018.04.030. View

Binder Gallimidi A, Fischman S, Revach B, Bulvik R, Maliutina A, Rubinstein A . Periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum promote tumor progression in an oral-specific chemical carcinogenesis model. Oncotarget. 2015; 6(26):22613-23. PMC: 4673186. DOI: 10.18632/oncotarget.4209. View

Farajzadeh Sheikh A, Masjedi Zadeh A, Saki M, Khani P, Hashemi S, Shahin Zadeh S . Detection of Streptococcus gallolyticus in colorectal cancer and inflammatory bowel disease patients compared to control group in southwest of Iran. Mol Biol Rep. 2020; 47(11):8361-8365. DOI: 10.1007/s11033-020-05807-7. View

10.

Yang Z, Ji G . -positive colorectal cancer. Oncol Lett. 2019; 18(2):975-982. PMC: 6607099. DOI: 10.3892/ol.2019.10433. View

11.

Matsumiya Y, Suenaga M, Ishikawa T, Kudo T, Nakagawa T, Okamoto K . Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer. Anaerobe. 2023; 84:102784. DOI: 10.1016/j.anaerobe.2023.102784. View

12.

Rubinstein M, Wang X, Liu W, Hao Y, Cai G, Han Y . Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin. Cell Host Microbe. 2013; 14(2):195-206. PMC: 3770529. DOI: 10.1016/j.chom.2013.07.012. View

13.

Dougherty M, Jobin C . Intestinal bacteria and colorectal cancer: etiology and treatment. Gut Microbes. 2023; 15(1):2185028. PMC: 10026918. DOI: 10.1080/19490976.2023.2185028. View

14.

Castellarin M, Warren R, Freeman J, Dreolini L, Krzywinski M, Strauss J . Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. Genome Res. 2011; 22(2):299-306. PMC: 3266037. DOI: 10.1101/gr.126516.111. View

15.

Mima K, Nishihara R, Qian Z, Cao Y, Sukawa Y, Nowak J . Fusobacterium nucleatum in colorectal carcinoma tissue and patient prognosis. Gut. 2015; 65(12):1973-1980. PMC: 4769120. DOI: 10.1136/gutjnl-2015-310101. View

16.

Song M, Chan A . Diet, Gut Microbiota, and Colorectal Cancer Prevention: A Review of Potential Mechanisms and Promising Targets for Future Research. Curr Colorectal Cancer Rep. 2018; 13(6):429-439. PMC: 5761320. DOI: 10.1007/s11888-017-0389-y. View

17.

Abu-Ghazaleh N, Chua W, Gopalan V . Intestinal microbiota and its association with colon cancer and red/processed meat consumption. J Gastroenterol Hepatol. 2020; 36(1):75-88. DOI: 10.1111/jgh.15042. View

18.

Shimpoh T, Hirata Y, Ihara S, Suzuki N, Kinoshita H, Hayakawa Y . Prevalence of -positive in Japanese patients with or without colorectal cancer. Gut Pathog. 2017; 9:35. PMC: 5468999. DOI: 10.1186/s13099-017-0185-x. View

19.

Zepeda-Rivera M, Minot S, Bouzek H, Wu H, Blanco-Miguez A, Manghi P . A distinct Fusobacterium nucleatum clade dominates the colorectal cancer niche. Nature. 2024; 628(8007):424-432. PMC: 11006615. DOI: 10.1038/s41586-024-07182-w. View

20.

Gholizadeh P, Eslami H, Kafil H . Carcinogenesis mechanisms of Fusobacterium nucleatum. Biomed Pharmacother. 2017; 89:918-925. DOI: 10.1016/j.biopha.2017.02.102. View