Effect of Fluorine Atoms and Piperazine Rings on Biotoxicity of Norfloxacin Analogues: Combined Experimental and Theoretical Study

Overview

Journal Environ Health (Wash)

Publisher American Chemical Society

Date 2024 Dec 26

PMID 39722844

Authors

Chuanxi Yang

Xiaoning Wang

Xinyan Zhao

Yongkun Wu

Jingyan Lin

Yuhan Zhao

Yiyong Xu

Kaipeng Sun

Chao Zhang

Ziheng Wan

Weihua Zhao

Yihua Xiao

Haofen Sun

Dong Chen

Wenping Dong

Tieyu Wang

Weiliang Wang

Affiliations

Soon will be listed here.

Abstract

To clarify the effect of the fluorine atom and piperazine ring on norfloxacin (NOR), NOR degradation products (NOR-DPs, P1-P8) were generated via UV combined with hydrogen peroxide (UV/HO) technology. NOR degradation did not significantly affect cytotoxicity of NOR against BV2, A549, HepG2, and Vero E6 cells. Compared with that of NOR, mutagenicity and median lethal concentration of P1-P8 in fathead minnow were increased, and bioaccumulation factor and oral median lethal dose of P1-P8 in rats were decreased. Molecular docking was used to evaluate the inhibitory effect of DNA gyrase A (gyrA) on NOR-DPs to determine the molecular-level mechanism and establish the structure-activity relationship. Results indicated that the most common amino acid residues were Ile13, Ser27, Val28, Gly31, Asp36, Arg46, Arg47, Asp157, and Gly340; hydrogen bonds and hydrophobic interactions played key roles in the inhibitory effect. Binding area (BA) decreased from 350.80 Å (NOR) to 346.21 Å (P1), and the absolute value of binding energy (|BE|) changed from 2.53 kcal/mol (NOR) to 2.54 kcal/mol (P1), indicating that the fluorine atom mainly affects BA. The piperazine ring clearly influenced BA and |BE|. "Yang ChuanXi Rules" were used to explain effects of molecular weight (MW), BA, |BE|, and sum of η + η (η: normalization of BA, η: normalization of |BE|) and predict biotoxicity of NOR-DPs based on half-maximum inhibitory concentration (IC), half-minimal inhibitory concentration (MIC), and half-minimal bactericidal concentration (MBC) values.

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