A Comparison of Digestive Strategies for With Different Diet Compositions: Digestive Enzyme Activities, Gut Microbiota, and Metabolites

Overview

Journal Ecol Evol

Date 2024 Dec 24

PMID 39717646

Authors

Ziyi Wang

Ruichen Wu

Yi Yang

Affiliations

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Abstract

Animal gut microbiota play important roles in host immunity, nutrient metabolism, and energy acquisition. The gut microbiota and its metabolic products interact with the host in many different ways, influencing gut homoeostasis and health. is an endemic species which displays special frugivorous behavior, and it has been observed consuming grapes. To explore the effects of grape intake on the gut microbiota and metabolites of , 16S rRNA sequencing and liquid chromatography mass spectrometry metabolomics were applied to investigate the gut microbiota and metabolite profiles of fed with mealworms (LC group) and a mixture of mealworms and grapes (FG group). Our results demonstrated that a notable shift in microbiota composition occurred, particularly in terms of an increase in the probiotic in the FG group. The metabolite analysis revealed a significant enrichment of the pathways related to glucose metabolism in the FG group. In addition, the digestive enzyme activity analysis showed that the α-amylase and cellulase activities in the FG group were significantly higher than those of the LC group, which was consistent with the food type. A strong correlation between diet, gut microbiota, and fecal metabolites was observed, which may imply that different diets promote the establishment of host intestinal adaptation strategies. Our study provides a theoretical basis for host health and the scientific captive breeding of the desert lizards .

Citing Articles

Correction to "A Comparison of Digestive Strategies for With Different Diet Compositions: Digestive Enzyme Activities, Gut Microbiota, and Metabolites"Wang Z, Wu R, Yang Y. A Comparison of Digestive Strategies for With Different Diet Compositions:....

Ecol Evol. 2025; 15(1):e70843.

PMID: 39781255 PMC: 11707255. DOI: 10.1002/ece3.70843.

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