The Combination of Super-Active Platelet Lysate and Acellular Amniotic Membrane Enhances Endometrial Receptivity, While Simultaneously Facilitating Endometrial Repair in Rats

Overview

Journal J Inflamm Res

Publisher Dove Medical Press

Date 2024 Dec 23

PMID 39713713

Authors

Huaying Wu

Yi Zhang

Chunxiang Liu

Xiaohan Tang

Liqun Wang

Lingqi Meng

Meisong Lu

Affiliations

Soon will be listed here.

Abstract

Purpose: To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.

Methods: The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury. We divided sixty-four rats into four groups: the Injury group (Control group), the AAM group, the sPL group, and the sPL-AAM group. Our study compared the endometrial thickness, gland count, and fibrotic area recovery in rats at 6 days and 18 days post-treatment. Immunohistochemistry was utilized to assess the expressions of CD34 and ANG. Additionally, we used ELISA to detect the levels of IL-6 and TNF-α, while Western Blot was employed to compare the expressions of CK19, Integrin β3, and TGF-β1. One month after the treatment, we evaluated and compared the pregnancy recovery among the groups.

Results: Compared to the Injury group, the sPL-AAM group demonstrated enhanced endometrial regeneration in rats at both 6 days and 18 days post-treatment, resulting in a favorable pregnancy outcome. This was achieved by promoting angiogenesis, suppressing the inflammatory response, and reducing fibrosis. The observed effects were superior to those of the sPL group alone. While sPL, when administered alone, showed some degree of endometrial restoration at 6 days post-treatment, its efficacy was diminished at 18 days post-treatment. The impact of AAM alone appeared inconspicuous compared to the injury group. This suggests that sPL serves as the primary agent in facilitating endometrial repair, while AAM functions as a carrier to extend the duration of sPL's effectiveness.

Conclusion: sPL-AAM can release effective cytokines, repair endometrial damage in rats, enhance endometrial receptivity, and ultimately improve pregnancy outcomes.

References

Nazari H, Heirani-Tabasi A, Esmaeili E, Kajbafzadeh A, Hassannejad Z, Boroomand S . Decellularized human amniotic membrane reinforced by MoS-Polycaprolactone nanofibers, a novel conductive scaffold for cardiac tissue engineering. J Biomater Appl. 2022; 36(9):1527-1539. DOI: 10.1177/08853282211063289. View

Xue S, Liu K, Parolini O, Wang Y, Deng L, Huang Y . Human acellular amniotic membrane implantation for lower third nasal reconstruction: a promising therapy to promote wound healing. Burns Trauma. 2018; 6:34. PMC: 6297938. DOI: 10.1186/s41038-018-0136-x. View

Zhang C, Chen C, Wang J, Wang Y, Wen S, Cao Y . An endometrial receptivity scoring system basing on the endometrial thickness, volume, echo, peristalsis, and blood flow evaluated by ultrasonography. Front Endocrinol (Lausanne). 2022; 13:907874. PMC: 9395662. DOI: 10.3389/fendo.2022.907874. View

Sanders F, Huang J, Alio Del Barrio J, Hamada S, McAlinden C . Amniotic membrane transplantation: structural and biological properties, tissue preparation, application and clinical indications. Eye (Lond). 2023; 38(4):668-679. PMC: 10920809. DOI: 10.1038/s41433-023-02777-5. View

Wang S, Shi C, Cai X, Wang Y, Chen X, Han H . Human Acellular Amniotic Matrix with Previously Seeded Umbilical Cord Mesenchymal Stem Cells Restores Endometrial Function in a Rat Model of Injury. Mediators Inflamm. 2021; 2021:5573594. PMC: 8438588. DOI: 10.1155/2021/5573594. View

Huang Z, Zhao Z, Lang J, Wang W, Fu Y, Wang W . Therapeutic Study of Thermosensitive Hydrogel Loaded with Super-Activated Platelet Lysate Combined with Core Decompression Technology for the Treatment of Femoral Head Necrosis. Stem Cells Int. 2021; 2021:7951616. PMC: 8257358. DOI: 10.1155/2021/7951616. View

Xu X, Kong D, Tian Y, Xie Y, Zhang J, Huang X . Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions. Taiwan J Obstet Gynecol. 2021; 60(6):1031-1037. DOI: 10.1016/j.tjog.2021.09.014. View

Ma J, Zhan H, Li W, Zhang L, Yun F, Wu R . Recent trends in therapeutic strategies for repairing endometrial tissue in intrauterine adhesion. Biomater Res. 2021; 25(1):40. PMC: 8611984. DOI: 10.1186/s40824-021-00242-6. View

Koot Y, Teklenburg G, Salker M, Brosens J, Macklon N . Molecular aspects of implantation failure. Biochim Biophys Acta. 2012; 1822(12):1943-50. DOI: 10.1016/j.bbadis.2012.05.017. View

10.

Zhang L, Li Y, Guan C, Tian S, Lv X, Li J . Therapeutic effect of human umbilical cord-derived mesenchymal stem cells on injured rat endometrium during its chronic phase. Stem Cell Res Ther. 2018; 9(1):36. PMC: 5810045. DOI: 10.1186/s13287-018-0777-5. View

11.

Li J, Liu N, Huang Z, Wang W, Hou D, Wang W . Intra-articular injection of loaded sPL sustained-release microspheres inhibits osteoarthritis and promotes cartilaginous repairs. J Orthop Surg Res. 2021; 16(1):646. PMC: 8557014. DOI: 10.1186/s13018-021-02777-9. View

12.

Chen X, Sun J, Li X, Mao L, Cui L, Bai W . Transplantation of oral mucosal epithelial cells seeded on decellularized and lyophilized amniotic membrane for the regeneration of injured endometrium. Stem Cell Res Ther. 2019; 10(1):107. PMC: 6429789. DOI: 10.1186/s13287-019-1179-z. View

13.

Huang Z, Wang Q, Zhang T, Fu Y, Wang W . Hyper-activated platelet lysates prevent glucocorticoid-associated femoral head necrosis by regulating autophagy. Biomed Pharmacother. 2021; 139:111711. DOI: 10.1016/j.biopha.2021.111711. View

14.

Zhuang D, Zhang Y, Lu H, Zhang H, Li T, Bi L . Super Activated Platelet Lysate, a Novel Autologous Platelet Lysate, Regulates the Expression of Inflammasome and Cytokine in the Experimental Periodontitis in Rats. Drug Des Devel Ther. 2020; 14:5535-5543. PMC: 7751324. DOI: 10.2147/DDDT.S289753. View

15.

Guo X, Lu H, Liu C, Zhang Y, Bi L . Effects of Super-Activated Platelet Lysate on Early Healing of Tooth Extraction Sockets in Rats. Drug Des Devel Ther. 2022; 16:2213-2227. PMC: 9289175. DOI: 10.2147/DDDT.S363766. View

16.

Chung T, Park M, Lee H, Kim K, Kim C, Choi H . Enhancement of Endometrial Receptivity by through Expressing LIF and Integrins. Evid Based Complement Alternat Med. 2019; 2019:7560631. PMC: 6885254. DOI: 10.1155/2019/7560631. View

17.

Lin P, Lee C, Chen Y, Cheng E, Huang C, Chen C . Factors Affecting the Potential Efficacy of Intrauterine Platelet-Rich Plasma Infusion on Thin Endometrium in Women with Recurrent Implantation Failure. J Pers Med. 2023; 13(9). PMC: 10532920. DOI: 10.3390/jpm13091419. View

18.

Salama N, Zaghlol S, Mohamed H, Kamar S . Suppression of the inflammation and fibrosis in Asherman syndrome rat model by mesenchymal stem cells: histological and immunohistochemical studies. Folia Histochem Cytobiol. 2020; 58(3):208-218. DOI: 10.5603/FHC.a2020.0024. View

19.

Han X, Ma Y, Lu X, Li W, Xia E, Li T . Transplantation of Human Adipose Stem Cells Using Acellular Human Amniotic Membrane Improves Angiogenesis in Injured Endometrial Tissue in a Rat Intrauterine Adhesion Model. Cell Transplant. 2020; 29:963689720952055. PMC: 7784510. DOI: 10.1177/0963689720952055. View

20.

Ouyang X, You S, Zhang Y, Zhang C, Zhang G, Shao X . Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020; 29(20):1346-1362. DOI: 10.1089/scd.2019.0246. View