Internal State of Vesicles Affects Higher Order State of Vesicle Assembly and Interaction

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Dec 23

PMID 39713690

Authors

Silvia Holler

Federica Casiraghi

Martin Michael Hanczyc

Affiliations

Soon will be listed here.

Abstract

Dynamic soft matter systems composed of functionalized vesicles and liposomes are typically produced and then manipulated through external means, including the addition of exogenous molecules. In biology, natural cells possess greater autonomy, as their internal states are continuously updated, enabling them to effect higher order properties of the system. Therefore, a conceptual and technical gap exists between the natural and artificial systems. We engineered functionalized vesicles to form multicore aggregates capable of self-assembly due to the presence of complementary ssDNA strands. A dynamic process was then triggered through an exogenously triggered on-demand release of an endogenously produced displacer molecule, resulting in multicore aggregate disassembly. This approach explores how internal states of vesicles can affect the external organization, demonstrating a very simple programmable strategy for assembly and then endogenous disassembly. This framework supports the exploration of larger and more complex multicore entities, opening a path toward community behavior and a higher degree of autonomy.

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