Effects of Cell-Free Fat Extract and Platelet-Rich Fibrin on Scar Maturation in an Experimental Rabbit Ear Wound Model

Overview

Journal Clin Cosmet Investig Dermatol

Publisher Dove Medical Press

Specialty Dermatology

Date 2024 Dec 23

PMID 39712941

Authors

Zhenni Wei

Mingliang Zhang

Minjian Chen

Yong Song

Yi Wang

Affiliations

Soon will be listed here.

Abstract

Background: Multiple methods have been used to treat hypertrophic scarring; however, an optimal treatment method remains to be established. We aimed to research and compare the effects of cell-free fat extract (CEFFE) and platelet-rich fibrin (PRF) on hypertrophic scar formation based on histomorphological analysis in this study.

Methods: Twelve rabbits were divided into four groups randomly. (CEFFE+PRF group, n=3; CEFFE group, n=3, PRF group, n=3 and Control group, n=3). After the ear hypertrophic scar model were established, the two ears of each rabbit in the four groups were injected with CEFFE 0.05 mL/cm + PRF 0.05 mL/cm, CEFFE 0.1 mL/cm, PRF 0.1 mL/cm, and saline 0.1 mL/cm, respectively. The scar elevation index and histological analysis using hematoxylin-eosin and Masson staining were evaluated after injection on day 40.

Results: The CEFFE+PRF group was significantly more effective in the prevention of pathological scar formation than the CEFFE-only, PRF-only, and control groups in terms of capillary count, collagen organization, fibroblast count, and scar elevation index (<0.05).

Discussion: CEFFE combined with PRF was the most effective treatment for the prevention of hypertrophic scar formation in our study.

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