Antioxidant and Antimicrobial Activities of Methanolic Extract and Its Phytochemical Characterization

Overview

Journal Open Life Sci

Specialty Biology

Date 2024 Dec 23

PMID 39711973

Authors

Helmy A Aamer

Saad F Elalem

Abdulaziz A Al-Askar

Omaima A Sharaf

Mahmoud A Gaber

Przemyslaw Kowalczewski

Said Behiry

Ahmed Abdelkhalek

Affiliations

Soon will be listed here.

Abstract

Methanolic extract from was investigated for its phytochemical content, antioxidant, and antimicrobial properties against phytopathogenic fungi and bacteria. Phytochemical analysis revealed the presence of saponin, tannins, and alkaloids with 1.25%, 18.8 mg catechin/g of extract, and 9.12%, respectively. Total flavonoid content was 20.8 mg quercetin equivalent/g while total phenolic content was 202 mg gallic acid equivalent/g. Antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay resulted in an IC value of 48.61 µg/mL, while the phosphomolybdenum method yielded a value of 215.43 mg ascorbic acid equivalent/g of extract. The highest phenolic acids detected in the extract were gallic acid (712.97 µg/g), syringic acid (742.7 µg/g), and caffeic acid (474.70 µg/g) according to high-performance liquid chromatography analysis. Palmitic acid (28.38%) dominated the fatty acids identified by gas chromatography-mass spectrometry, while stigmasterol (8.34%) was the most abundant steroid. At a concentration of 3 mg/mL, the extract showed strong antibacterial activity against (10.50 mm), (9.93 mm), and (8.37 mm). Additionally, the extract significantly suppressed fungal growth of (38.22%) and (33.56%) but showed lower activity toward (13.33%) at 5 mg/mL. In conclusion, extract exhibited promising antioxidant and antimicrobial properties, making it a potential candidate for further exploration in agricultural applications.

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