Hydrogen Gas Inhalation Prior to High-intensity Training Reduces Attenuation of Nitric Oxide Bioavailability in Male Rugby Players

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2024 Dec 20

PMID 39703911

Authors

Yuqi Zhao

Chaoqun Li

Shi Zhou

Zhiguang Xu

Xin Huang

Li Wen

Affiliations

Soon will be listed here.

Abstract

Background: Inhalation of hydrogen gas (H) as an antioxidant supplement may alleviate exercise-induced oxidative damage and protect post-exercise hydrogen peroxide signaling, which may help mediate beneficial exercise adaptation. The aims of this study were to determine the effects of H inhalation on plasma nitric oxide (NO) level and its synthesis precursor in professional athletes.

Methods: A randomized, placebo-controlled, double-blind, crossover trial was conducted with professional male rugby players for 3 weeks. Participants underwent 1 week of H supplementation and 1 week of placebo treatment prior to daily sessions of high-intensity exercise training, separated by 1 week of low-intensity training as a washout.

Results: Two-way (supplementation and time) repeated-measures analyses of variance showed that NO, L-arginine, and tetrahydrobiopterin levels in the H inhalation group were significantly higher than those in the placebo group after exercise (D6) and remained higher after 24 h of rest (D7). Levels of hydroxydeoxyguanosine and interleukin 6 were lower in the H inhalation week than in the placebo week on D6 and D7. In addition, total antioxidant levels were significantly higher with H inhalation than with placebo.

Significance: These results suggest that H inhalation helps to maintain NO signaling after exercise and to alleviate inflammation and oxidative stress induced by high-intensity exercise training in professional athletes.

References

Dobashi S, Takeuchi K, Koyama K . Hydrogen-rich water suppresses the reduction in blood total antioxidant capacity induced by 3 consecutive days of severe exercise in physically active males. Med Gas Res. 2020; 10(1):21-26. PMC: 7871940. DOI: 10.4103/2045-9912.279979. View

Waehre T, Yndestad A, Smith C, Haug T, Haugen Tunheim S, Gullestad L . Increased expression of interleukin-1 in coronary artery disease with downregulatory effects of HMG-CoA reductase inhibitors. Circulation. 2004; 109(16):1966-72. DOI: 10.1161/01.CIR.0000125700.33637.B1. View

Hayashi Y, Sawa Y, Nishimura M, Fukuyama N, Ichikawa H, Ohtake S . Peroxynitrite, a product between nitric oxide and superoxide anion, plays a cytotoxic role in the development of post-bypass systemic inflammatory response. Eur J Cardiothorac Surg. 2004; 26(2):276-80. DOI: 10.1016/j.ejcts.2004.03.033. View

Merry T, Ristow M . Do antioxidant supplements interfere with skeletal muscle adaptation to exercise training?. J Physiol. 2015; 594(18):5135-47. PMC: 5023714. DOI: 10.1113/JP270654. View

Zaryski C, Smith D . Training principles and issues for ultra-endurance athletes. Curr Sports Med Rep. 2005; 4(3):165-70. DOI: 10.1097/01.csmr.0000306201.49315.73. View

Fuhrmann D, Brune B . Mitochondrial composition and function under the control of hypoxia. Redox Biol. 2017; 12:208-215. PMC: 5333533. DOI: 10.1016/j.redox.2017.02.012. View

Roque F, Briones A, Garcia-Redondo A, Galan M, Martinez-Revelles S, Avendano M . Aerobic exercise reduces oxidative stress and improves vascular changes of small mesenteric and coronary arteries in hypertension. Br J Pharmacol. 2012; 168(3):686-703. PMC: 3579288. DOI: 10.1111/j.1476-5381.2012.02224.x. View

Cadenas S . ROS and redox signaling in myocardial ischemia-reperfusion injury and cardioprotection. Free Radic Biol Med. 2018; 117:76-89. DOI: 10.1016/j.freeradbiomed.2018.01.024. View

Van Tassell B, Valle Raleigh J, Abbate A . Targeting interleukin-1 in heart failure and inflammatory heart disease. Curr Heart Fail Rep. 2014; 12(1):33-41. DOI: 10.1007/s11897-014-0231-7. View

10.

Ignarro L . Biological actions and properties of endothelium-derived nitric oxide formed and released from artery and vein. Circ Res. 1989; 65(1):1-21. DOI: 10.1161/01.res.65.1.1. View

11.

Peters P, Alessio H, Hagerman A, Ashton T, Nagy S, Wiley R . Short-term isometric exercise reduces systolic blood pressure in hypertensive adults: possible role of reactive oxygen species. Int J Cardiol. 2005; 110(2):199-205. DOI: 10.1016/j.ijcard.2005.07.035. View

12.

Gliemann L, Nyberg M, Hellsten Y . Nitric oxide and reactive oxygen species in limb vascular function: what is the effect of physical activity?. Free Radic Res. 2013; 48(1):71-83. DOI: 10.3109/10715762.2013.835045. View

13.

Powers S, Deminice R, Ozdemir M, Yoshihara T, Bomkamp M, Hyatt H . Exercise-induced oxidative stress: Friend or foe?. J Sport Health Sci. 2020; 9(5):415-425. PMC: 7498668. DOI: 10.1016/j.jshs.2020.04.001. View

14.

Suzuki K, Tominaga T, Ruhee R, Ma S . Characterization and Modulation of Systemic Inflammatory Response to Exhaustive Exercise in Relation to Oxidative Stress. Antioxidants (Basel). 2020; 9(5). PMC: 7278761. DOI: 10.3390/antiox9050401. View

15.

Sies H, Jones D . Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nat Rev Mol Cell Biol. 2020; 21(7):363-383. DOI: 10.1038/s41580-020-0230-3. View

16.

Aoki K, Nakao A, Adachi T, Matsui Y, Miyakawa S . Pilot study: Effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes. Med Gas Res. 2012; 2:12. PMC: 3395574. DOI: 10.1186/2045-9912-2-12. View

17.

Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U . Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013; 99(1):55-64. DOI: 10.1093/cvr/cvt080. View

18.

Heinonen I, Ilkka H, Saltin B, Bengt S, Kemppainen J, Jukka K . Skeletal muscle blood flow and oxygen uptake at rest and during exercise in humans: a pet study with nitric oxide and cyclooxygenase inhibition. Am J Physiol Heart Circ Physiol. 2011; 300(4):H1510-7. DOI: 10.1152/ajpheart.00996.2010. View

19.

Ashor A, Lara J, Siervo M, Celis-Morales C, Oggioni C, Jakovljevic D . Exercise modalities and endothelial function: a systematic review and dose-response meta-analysis of randomized controlled trials. Sports Med. 2014; 45(2):279-96. DOI: 10.1007/s40279-014-0272-9. View

20.

Li H, Forstermann U . Uncoupling of endothelial NO synthase in atherosclerosis and vascular disease. Curr Opin Pharmacol. 2013; 13(2):161-7. DOI: 10.1016/j.coph.2013.01.006. View