Asperuloside Alleviates Osteoporosis by Promoting Autophagy and Regulating Nrf2 Activation

Overview

Journal J Orthop Surg Res

Publisher Biomed Central

Specialty Orthopedics

Date 2024 Dec 20

PMID 39702357

Authors

Fenglan Huang

Yiteng Wang

Jinzhu Liu

Ye Cheng

Xiaonan Zhang

Haoli Jiang

Affiliations

Soon will be listed here.

Abstract

Background: Osteoporosis is a metabolic bone disease that has a common occurrence in postmenopausal women. Asperuloside (ASP) has been reported to exert anti-inflammatory and anti-oxidative effects in numerous diseases, such as rheumatoid arthritis and acute lung injury. However, whether ASP plays a role in osteoporosis has not been addressed.

Methods: In vivo, ovariectomy (OVX) was used to induce mouse osteoporosis. Then, the mice were treated with 20 and 40 mg/kg ASP. In vitro, MC3T3-E1 cells were treated with 0, 1, 10, 20, 40 and 80 µM ASP. We chose 20 and 40 µM for further experiments due to no significant effects on cell viability.

Results: The data indicated that ASP reduced osteoporosis in OVX mice and promoted osteogenic differentiation and mineralization in MC3T3-E1 cells. In addition, we explored that ASP protected against osteoporosis via inducing autophagy and activating Nrf2.

Conclusion: ASP alleviates OVX-induced osteoporosis by promoting autophagy and regulating Nrf2 activation.

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