MiRNA‑22‑3p Inhibits Cell Viability and Metastasis of Nasopharyngeal Carcinoma by Targeting FOXP1

Overview

Journal Oncol Lett

Specialty Oncology

Date 2024 Dec 19

PMID 39697977

Authors

Ying Jin

Zhijun Wang

Yuanshan Liang

Yiting Jiang

Fayang Yuan

Tian Zhang

Affiliations

Soon will be listed here.

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor with a high incidence rate in certain regions. MicroRNA (miRNA/miR)-22-3p is implicated in the regulation of tumorigenesis and progression. However, the biological role of miRNA-22-3p in the progression of NPC remains unclear. The present study aimed to assess the effects of miRNA-22-3p overexpression on the cell viability and migration of NPC cells. The cell viability and migration of HK-1 cells was evaluated using Transwell, wound healing and Cell Counting Kit-8 assays. To assess the epithelial-mesenchymal transition ability of NPC cells, the expression of E-cadherin, vimentin and N-cadherin was evaluated using western blot analysis. The results revealed expression of miRNA-22-3p was significantly decreased in NPC tissues compared with para-cancerous tissues. Decreased expression of miRNA-22-3p was also observed in NPC cell lines (C666-1 and HK-1). The overexpression of miRNA-22-3p reduced HK-1 cell viability and migration. In addition, a dual luciferase reporter assay revealed that miRNA-22-3p functioned as a molecular sponge for forkhead box protein 1 (FOXP1). Notably, FOXP1 overexpression counteracted the suppressive effects induced by transfection with miRNA-22-3p mimic on HK-1 cell viability and migration. Therefore, these data indicate that miRNA-22-3p may be a clinically valuable biomarker for the therapy of NPC.

References

Djuranovic S, Nahvi A, Green R . A parsimonious model for gene regulation by miRNAs. Science. 2011; 331(6017):550-3. PMC: 3955125. DOI: 10.1126/science.1191138. View

Han S, Wu X, Wan L, Zeng Q, Li J, Liu Z . FOXP1 expression predicts polymorphic histology and poor prognosis in gastric mucosa-associated lymphoid tissue lymphomas. Dig Surg. 2009; 26(2):156-62. DOI: 10.1159/000212058. View

Barrans S, Fenton J, Banham A, Owen R, Jack A . Strong expression of FOXP1 identifies a distinct subset of diffuse large B-cell lymphoma (DLBCL) patients with poor outcome. Blood. 2004; 104(9):2933-5. DOI: 10.1182/blood-2004-03-1209. View

Jia X, Yang M, Hu W, Cai S . Overexpression of miRNA-22-3p attenuates osteoporosis by targeting MAPK14. Exp Ther Med. 2021; 22(1):692. PMC: 8112124. DOI: 10.3892/etm.2021.10124. View

Fan D, Zhao Y, Qi H, Hou J, Zhang T . MiRNA-506 presents multiple tumor suppressor activities by targeting EZH2 in nasopharyngeal carcinoma. Auris Nasus Larynx. 2020; 47(4):632-642. DOI: 10.1016/j.anl.2019.12.007. View

Tang Y, Jin X, Xiang Y, Chen Y, Shen C, Zhang Y . The lncRNA MALAT1 protects the endothelium against ox-LDL-induced dysfunction via upregulating the expression of the miR-22-3p target genes CXCR2 and AKT. FEBS Lett. 2015; 589(20 Pt B):3189-96. DOI: 10.1016/j.febslet.2015.08.046. View

Guan S, Wei J, Huang L, Wu L . Chemotherapy and chemo-resistance in nasopharyngeal carcinoma. Eur J Med Chem. 2020; 207:112758. DOI: 10.1016/j.ejmech.2020.112758. View

Patzelt T, Keppler S, Gorka O, Thoene S, Wartewig T, Reth M . Foxp1 controls mature B cell survival and the development of follicular and B-1 B cells. Proc Natl Acad Sci U S A. 2018; 115(12):3120-3125. PMC: 5866538. DOI: 10.1073/pnas.1711335115. View

Shi C, Sakuma M, Mooroka T, Liscoe A, Gao H, Croce K . Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function. Blood. 2008; 112(12):4699-711. PMC: 2597137. DOI: 10.1182/blood-2008-01-137018. View

10.

Golson M, Kaestner K . Fox transcription factors: from development to disease. Development. 2016; 143(24):4558-4570. PMC: 5201025. DOI: 10.1242/dev.112672. View

11.

Ijichi N, Ikeda K, Horie-Inoue K, Inoue S . FOXP1 and estrogen signaling in breast cancer. Vitam Horm. 2013; 93:203-12. DOI: 10.1016/B978-0-12-416673-8.00006-X. View

12.

Chen L, Heikkinen L, Wang C, Yang Y, Sun H, Wong G . Trends in the development of miRNA bioinformatics tools. Brief Bioinform. 2018; 20(5):1836-1852. PMC: 7414524. DOI: 10.1093/bib/bby054. View

13.

Koon H, Ippolito G, Banham A, Tucker P . FOXP1: a potential therapeutic target in cancer. Expert Opin Ther Targets. 2007; 11(7):955-65. PMC: 4282158. DOI: 10.1517/14728222.11.7.955. View

14.

Fox S, Brown P, Han C, Ashe S, Leek R, Harris A . Expression of the forkhead transcription factor FOXP1 is associated with estrogen receptor alpha and improved survival in primary human breast carcinomas. Clin Cancer Res. 2004; 10(10):3521-7. DOI: 10.1158/1078-0432.CCR-03-0461. View

15.

Zhu Q, Zhang Q, Gu M, Zhang K, Xia T, Zhang S . upregulation suppresses autophagy and accelerates malignant phenotype in nasopharyngeal carcinoma. Autophagy. 2020; 17(7):1667-1683. PMC: 8354606. DOI: 10.1080/15548627.2020.1781368. View

16.

Chen Y, Chan A, Le Q, Blanchard P, Sun Y, Ma J . Nasopharyngeal carcinoma. Lancet. 2019; 394(10192):64-80. DOI: 10.1016/S0140-6736(19)30956-0. View

17.

Chang E, Ye W, Zeng Y, Adami H . The Evolving Epidemiology of Nasopharyngeal Carcinoma. Cancer Epidemiol Biomarkers Prev. 2021; 30(6):1035-1047. DOI: 10.1158/1055-9965.EPI-20-1702. View

18.

Zhao Y, Chen X, Jing M, Du H, Zeng Y . Expression of miRNA-146a in nasopharyngeal carcinoma is upregulated by Epstein-Barr virus latent membrane protein 1. Oncol Rep. 2012; 28(4):1237-42. DOI: 10.3892/or.2012.1933. View

19.

Li C, Li X, Wang H, Guo X, Xue J, Wang X . MicroRNA-22-3p and MicroRNA-149-5p Inhibit Human Hepatocellular Carcinoma Cell Growth and Metastasis Properties by Regulating Methylenetetrahydrofolate Reductase. Curr Issues Mol Biol. 2022; 44(2):952-962. PMC: 8928992. DOI: 10.3390/cimb44020063. View

20.

Hill M, Tran N . miRNA interplay: mechanisms and consequences in cancer. Dis Model Mech. 2021; 14(4). PMC: 8077553. DOI: 10.1242/dmm.047662. View