Accurate, Affordable, and Easy Electrochemical Detection of Ascorbic Acid in Fresh Fruit Juices and Pharmaceutical Samples Using an Electroactive Gelatin Sulfonamide

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2024 Dec 19

PMID 39697253

Authors

Hend S Magar

Asmaa M Fahim

M S Hashem

Affiliations

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Abstract

In this study, we demonstrated how to design and construct a highly specific and sensitive sensor capable of rapidly and accurately detecting ascorbic acid (AA). A sulfonamide derivative (S) acting as a novel monomer was synthesized through an aldol condensation reaction. Subsequently, a free radical-mediated grafting polymerization approach was used to create a new generation of gelatin (Gel) grafted with poly sulfonamide derivative (Gel-g-PS). The graft percentage (GP%) was 60 ± 0.5% with a conversion rate of 98.3%. Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) were utilized to confirm the formation of Gel-g-PS. The developed gelatin sulfonamide modified screen printed electrode (Gel-g-PS/SPE) was employed for the determination of ascorbic acid (AA) in fruit juices and pharmaceutical samples. Gel-g-PS/SPE showed excellent electrochemical catalytic activities toward AA oxidation compared to bare (unmodified) SPE. Ascorbic acid displayed a sensitive oxidation peak at 0.35 V using the differential pulse voltammetry technique. Under optimized experimental conditions, the two linear ranges for AA detection were obtained to be from 0.2-5 ppb and 20-600 ppb, with a limit of detection (LoD) of 0.03 ppb and a limit of quantification (LoQ) of 0.11 ppb. The proposed Gel-g-PS modified SPE surface demonstrated good selectivity, stability, reproducibility, and repeatability as well as a good recovery rate in fresh fruit juices and pharmaceutical samples.

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