Chitosan-casein As Novel Drug Delivery System for Transferring Phyllanthus Emblica to Inhibit Pseudomonas Aeruginosa

Overview

Journal BMC Biotechnol

Publisher Biomed Central

Specialty Biotechnology

Date 2024 Dec 19

PMID 39696307

Authors

Helia Ramezani

Hossein Sazegar

Leila Rouhi

Affiliations

Soon will be listed here.

Abstract

This study investigated the ability of Phyllanthus emblica encapsulated within chitosan-coated casein (CS-casein-Amla) nanoparticles to inhibit the growth of multi-drug-resistant Pseudomonas aeruginosa (P. aeruginosa) bacteria and prevent the formation of biofilms. The MDR strains underwent screening, and the morphological characteristics of the resulting nanoparticles were assessed using SEM, DLS, and FTIR. In addition, the efficacy of encapsulation, stability, and drug release were evaluated. The PpgL, BdlA, and GacA biofilm gene transcription quantities were quantified by quantitative real-time PCR. Simultaneously, the nanoparticles were assessed for their antibacterial and cytotoxic effects using the well diffusion and MTT procedures. CS-casein-Amla nanoparticles with a size of 500.73 ± 13 nm, encapsulation efficiency of 76.33 ± 0.81%, and stability for 60 days at 4 °C (Humidity 30%) were created. The biological analysis revealed that CS-casein-Amla nanoparticles exhibited strong antibacterial properties. This was shown by their capacity to markedly reduce the transcription of PpgL, BdlA, and GacA biofilm genes at a statistically significant value of p ≤ 0.01. The nanoparticles demonstrated decreased antibiotic resistance compared to unbound Amla and CS-casein. Compared to Amla, CS-casein-Amla nanoparticles showed very little toxicity against HDF cells at dosages ranging from 1.56 to 100 µg/mL (p ≤ 0.01). The results highlight the potential of CS-casein-Amla nanoparticles as a significant advancement in combating highly resistant P. aeruginosa. The powerful antibacterial properties of CS-casein-Amla nanoparticles against P. aeruginosa MDR strains, which are highly resistant pathogens of great concern, may catalyze the development of novel antibacterial research approaches.

References

Qin S, Xiao W, Zhou C, Pu Q, Deng X, Lan L . Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics. Signal Transduct Target Ther. 2022; 7(1):199. PMC: 9233671. DOI: 10.1038/s41392-022-01056-1. View

de Sousa T, Hebraud M, Alves O, Costa E, Maltez L, Pereira J . Study of Antimicrobial Resistance, Biofilm Formation, and Motility of Derived from Urine Samples. Microorganisms. 2023; 11(5). PMC: 10224020. DOI: 10.3390/microorganisms11051345. View

Botelho J, Grosso F, Peixe L . Antibiotic resistance in Pseudomonas aeruginosa - Mechanisms, epidemiology and evolution. Drug Resist Updat. 2019; 44:100640. DOI: 10.1016/j.drup.2019.07.002. View

Alves M, Bouchami O, Tavares A, Cordoba L, Santos C, Miragaia M . New Insights into Antibiofilm Effect of a Nanosized ZnO Coating against the Pathogenic Methicillin Resistant Staphylococcus aureus. ACS Appl Mater Interfaces. 2017; 9(34):28157-28167. DOI: 10.1021/acsami.7b02320. View

Deena S, Kumar G, Vickram A, Singhania R, Dong C, Rohini K . Efficiency of various biofilm carriers and microbial interactions with substrate in moving bed-biofilm reactor for environmental wastewater treatment. Bioresour Technol. 2022; 359:127421. DOI: 10.1016/j.biortech.2022.127421. View

Liu X, Xia W, Jiang Q, Xu Y, Yu P . Effect of kojic acid-grafted-chitosan oligosaccharides as a novel antibacterial agent on cell membrane of gram-positive and gram-negative bacteria. J Biosci Bioeng. 2015; 120(3):335-9. DOI: 10.1016/j.jbiosc.2015.01.010. View

Sadiq U, Gill H, Chandrapala J . Casein Micelles as an Emerging Delivery System for Bioactive Food Components. Foods. 2021; 10(8). PMC: 8392355. DOI: 10.3390/foods10081965. View

Yin R, Cheng J, Wang J, Li P, Lin J . Treatment of infectious biofilms: Challenges and strategies. Front Microbiol. 2022; 13:955286. PMC: 9459144. DOI: 10.3389/fmicb.2022.955286. View

Mu H, Guo F, Niu H, Liu Q, Wang S, Duan J . Chitosan improves anti-biofilm efficacy of gentamicin through facilitating antibiotic penetration. Int J Mol Sci. 2014; 15(12):22296-308. PMC: 4284708. DOI: 10.3390/ijms151222296. View

10.

Sahariah P, Masson M, Meyer R . Quaternary Ammoniumyl Chitosan Derivatives for Eradication of Staphylococcus aureus Biofilms. Biomacromolecules. 2018; 19(9):3649-3658. DOI: 10.1021/acs.biomac.8b00718. View

11.

Mangla B, Javed S, Sultan M, Ahsan W, Aggarwal G, Kohli K . Nanocarriers-Assisted Needle-Free Vaccine Delivery Through Oral and Intranasal Transmucosal Routes: A Novel Therapeutic Conduit. Front Pharmacol. 2022; 12:757761. PMC: 8787087. DOI: 10.3389/fphar.2021.757761. View

12.

Siddhardha B, Pandey U, Kaviyarasu K, Pala R, Syed A, Bahkali A . Chrysin-Loaded Chitosan Nanoparticles Potentiates Antibiofilm Activity against . Pathogens. 2020; 9(2). PMC: 7168315. DOI: 10.3390/pathogens9020115. View

13.

Xu J, Lin Q, Sheng M, Ding T, Li B, Gao Y . Antibiofilm Effect of Cinnamaldehyde-Chitosan Nanoparticles against the Biofilm of . Antibiotics (Basel). 2022; 11(10). PMC: 9598764. DOI: 10.3390/antibiotics11101403. View

14.

Chevalier S, Bouffartigues E, Bazire A, Tahrioui A, Duchesne R, Tortuel D . Extracytoplasmic function sigma factors in Pseudomonas aeruginosa. Biochim Biophys Acta Gene Regul Mech. 2018; 1862(7):706-721. DOI: 10.1016/j.bbagrm.2018.04.008. View

15.

Shekunov B, Chattopadhyay P, Tong H, Chow A . Particle size analysis in pharmaceutics: principles, methods and applications. Pharm Res. 2006; 24(2):203-27. DOI: 10.1007/s11095-006-9146-7. View

16.

Elzoghby A, El-Fotoh W, Elgindy N . Casein-based formulations as promising controlled release drug delivery systems. J Control Release. 2011; 153(3):206-16. DOI: 10.1016/j.jconrel.2011.02.010. View

17.

Rashki S, Safardoust-Hojaghan H, Mirzaei H, K Abdulsahib W, Mahdi M, Salavati-Niasari M . Delivery LL37 by chitosan nanoparticles for enhanced antibacterial and antibiofilm efficacy. Carbohydr Polym. 2022; 291:119634. DOI: 10.1016/j.carbpol.2022.119634. View

18.

Sharma G, Rao S, Bansal A, Dang S, Gupta S, Gabrani R . Pseudomonas aeruginosa biofilm: potential therapeutic targets. Biologicals. 2013; 42(1):1-7. DOI: 10.1016/j.biologicals.2013.11.001. View

19.

Yan D, Li Y, Liu Y, Li N, Zhang X, Yan C . Antimicrobial Properties of Chitosan and Chitosan Derivatives in the Treatment of Enteric Infections. Molecules. 2021; 26(23). PMC: 8659174. DOI: 10.3390/molecules26237136. View

20.

Mohanty S, Mishra S, Jena P, Jacob B, Sarkar B, Sonawane A . An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles. Nanomedicine. 2011; 8(6):916-24. DOI: 10.1016/j.nano.2011.11.007. View