The GTE4-EML Chromatin Reader Complex Concurrently Recognizes Histone Acetylation and H3K4 Trimethylation in Arabidopsis

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2024 Dec 18

PMID 39692581

Authors

Feng Qian

Qiang-Qiang Zhao

Jin-Xing Zhou

Dan-Yang Yuan

Zhen-Zhen Liu

Yin-Na Su

Lin Li

She Chen

Xin-Jian He

Affiliations

Soon will be listed here.

Abstract

Histone acetylation and H3K4 trimethylation (H3K4me3) are associated with active transcription. However, how they cooperate to regulate transcription in plants remains largely unclear. Our study revealed that GLOBAL TRANSCRIPTION FACTOR GROUP E 4 (GTE4) binds to acetylated histones and forms a complex with the functionally redundant H3K4me3-binding EMSY-like proteins EML1 or EML2 (EML1/2) in Arabidopsis thaliana. The eml1 eml2 (eml1/2) double mutant exhibits a similar morphological phenotype to gte4, and most of the differentially expressed genes in gte4 were coregulated in eml1/2. Through chromatin immunoprecipitation followed by deep sequencing, we found that GTE4 and EML2 co-occupy protein-coding genes enriched with both histone acetylation and H3K4me3, exerting a synergistic effect on the association of the GTE4-EML complex with chromatin. The association of GTE4 with chromatin requires both its bromodomain and EML-interacting domain. This study identified a complex and uncovered how it concurrently recognizes histone acetylation and H3K4me3 to facilitate gene transcription at the whole-genome level in Arabidopsis.

Citing Articles

Unraveled: The role of Arabidopsis GTE4-EML in transcription via two distinct histone modifications.

Ng P Plant Cell. 2025; 37(1.

PMID: 39761353 PMC: 11749275. DOI: 10.1093/plcell/koaf009.

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