Blastocyst Complementation-based Rat-derived Heart Generation Reveals Cardiac Anomaly Barriers to Interspecies Chimera Development

Overview

Journal iScience

Publisher Cell Press

Date 2024 Dec 17

PMID 39687030

Authors

Shunsuke Yuri

Norie Arisawa

Kohei Kitamuro

Ayako Isotani

Affiliations

Soon will be listed here.

Abstract

The use of pluripotent stem cells (PSCs) to generate functional organs via blastocyst complementation is a cutting-edge strategy in regenerative medicine. However, existing models that use this method for heart generation do not meet expectations owing to the complexity of heart development. Here, we investigated a Mesp1/2 deficient mouse model, which is characterized by abnormalities in the cardiac mesodermal cells. The injection of either mouse or rat PSCs into Mesp1/2 deficient mouse blastocysts led to successful heart generation. In chimeras, the resulting hearts were predominantly composed of rat cells; however, their functionality was limited to the embryonic developmental stage on day 12.5. These results present the functional limitation of the xenogeneic heart, which poses a significant challenge to the development in mouse-rat chimeras.

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