BC99 Enhances Intestinal Barrier Function by Modulating Butyrate Formation to Alleviate Acute Alcohol Intoxication in Rats

Overview

Journal Nutrients

Date 2024 Dec 17

PMID 39683538

Authors

Cheng Li

Shirui Zhai

Mengyao Duan

Li Cao

Jie Zhang

Yao Wang

Ying Wu

Shaobin Gu

Affiliations

Soon will be listed here.

Abstract

Probiotics have great potential in improving acute alcohol intoxication. The aim of this study was to investigate the mitigating effect and mechanism of action of BC99 on acute alcohol intoxication (AAI) in SD rats. BC99 was divided into different doses administered by gavage to rats, and a rat model of acute alcohol intoxication was established by multiple gavages of excess alcohol. Our study demonstrated that BC99 intervention significantly prolonged the latency period of intoxication; significantly attenuated alcohol-induced lipid elevation, liver injury, hepatic inflammation, and intestinal barrier damage; and lowered plasma endotoxin (LPS) levels in rats. In addition, BC99 could effectively restore the balance of intestinal flora, increase the abundance of _, _, , and , and thus increase the content of intestinal short-chain fatty acids (SCFAs), especially butyric acid. Moreover, we demonstrated through sodium butyrate validation experiments that butyrate could attenuate intestinal barrier damage and reduce the diffusion of LPS, thereby reducing liver inflammation. In conclusion, BC99 ameliorates acute alcohol intoxication in rats by increasing the abundance of butyrate-producing genera and thereby increasing butyrate abundance to alleviate intestinal barrier injury.

Citing Articles

The Improvement Effects of BC99 on Liver Function and Gut Microbiota of Long-Term Alcohol Drinkers: A Randomized Double-Blind Clinical Trial.

Zhang J, Li C, Duan M, Qu Z, Wang Y, Dong Y Nutrients. 2025; 17(2).

PMID: 39861457 PMC: 11769147. DOI: 10.3390/nu17020320.

BC99 Attenuates Oxidative Stress Induced by Acute Alcoholic Liver Injury via Nrf2/SKN-1 Pathway and Liver Metabolism Regulation.

Wu Y, Li C, Gao Y, Zhang J, Dong Y, Zhao L Antioxidants (Basel). 2025; 14(1.

PMID: 39857451 PMC: 11760874. DOI: 10.3390/antiox14010117.

References

Zhang B, Xu Y, Zhao C, Zhang Y, Lv H, Ji X . Protective effects of bioactive peptides in foxtail millet protein hydrolysates against experimental colitis in mice. Food Funct. 2022; 13(5):2594-2605. DOI: 10.1039/d1fo02482e. View

Rao R . Endotoxemia and gut barrier dysfunction in alcoholic liver disease. Hepatology. 2009; 50(2):638-44. PMC: 6209509. DOI: 10.1002/hep.23009. View

Tithi T, Tahsin M, Anjum J, Zaman T, Aktar F, Bahar N . An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity. PLoS One. 2023; 18(9):e0291125. PMC: 10503776. DOI: 10.1371/journal.pone.0291125. View

Kleczkowska P, Sulejczak D, Zaremba M . Advantages and disadvantages of disulfiram coadministered with popular addictive substances. Eur J Pharmacol. 2021; 904:174143. DOI: 10.1016/j.ejphar.2021.174143. View

Arpaia N, Campbell C, Fan X, Dikiy S, van der Veeken J, deRoos P . Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation. Nature. 2013; 504(7480):451-5. PMC: 3869884. DOI: 10.1038/nature12726. View

Mishra J, Stubbs M, Kuang L, Vara N, Kumar P, Kumar N . Inflammatory Bowel Disease Therapeutics: A Focus on Probiotic Engineering. Mediators Inflamm. 2022; 2022:9621668. PMC: 8786545. DOI: 10.1155/2022/9621668. View

Yu Q, Ji Z . Fine nursing intervention relieves the clinical symptoms and decreases the adverse events in acute alcoholism patients. Am J Transl Res. 2021; 13(10):11671-11679. PMC: 8581946. View

Hill C, Guarner F, Reid G, Gibson G, Merenstein D, Pot B . Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol. 2014; 11(8):506-14. DOI: 10.1038/nrgastro.2014.66. View

Liu Z, Liu T, Zhang Z, Fan Y . regulates gut microbiota and ameliorates the alcoholic-associated liver disease in mice. Front Microbiol. 2024; 15:1337185. PMC: 11002907. DOI: 10.3389/fmicb.2024.1337185. View

10.

Li H, Shi J, Zhao L, Guan J, Liu F, Huo G . KLDS1.0344 and KLDS1.0901 Mixture Prevents Chronic Alcoholic Liver Injury in Mice by Protecting the Intestinal Barrier and Regulating Gut Microbiota and Liver-Related Pathways. J Agric Food Chem. 2020; 69(1):183-197. DOI: 10.1021/acs.jafc.0c06346. View

11.

Xiao C, Jia R, Li X, Zhao M, Liao W, Zhao S . peptides alleviated alcoholic liver injury the gut-liver axis. Food Funct. 2024; 15(13):7124-7135. DOI: 10.1039/d4fo01070a. View

12.

Geng X, Du X, Wang W, Zhang C, Liu X, Qu Y . Confined Cascade Metabolic Reprogramming Nanoreactor for Targeted Alcohol Detoxification and Alcoholic Liver Injury Management. ACS Nano. 2023; 17(8):7443-7455. DOI: 10.1021/acsnano.2c12075. View

13.

Anand S, Sharma A, Singh N, Kakkar P . Activation of autophagic flux via LKB1/AMPK/mTOR axis against xenoestrogen Bisphenol-A exposure in primary rat hepatocytes. Food Chem Toxicol. 2020; 141:111314. DOI: 10.1016/j.fct.2020.111314. View

14.

Gan Y, Chen X, Yi R, Zhao X . Antioxidative and Anti-Inflammatory Effects of ZS62 on Alcohol-Induced Subacute Hepatic Damage. Oxid Med Cell Longev. 2021; 2021:7337988. PMC: 8668337. DOI: 10.1155/2021/7337988. View

15.

. Global burden of 87 risk factors in 204 countries and territories, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet. 2020; 396(10258):1223-1249. PMC: 7566194. DOI: 10.1016/S0140-6736(20)30752-2. View

16.

Taheri M, Amiri-Farahani L, Haghani S, Shokrpour M, Shojaii A . The effect of olive cream on pain and healing of caesarean section wounds: a randomised controlled clinical trial. J Wound Care. 2022; 31(3):244-253. DOI: 10.12968/jowc.2022.31.3.244. View

17.

Dukic M, Radonjic T, Jovanovic I, Zdravkovic M, Todorovic Z, Kraisnik N . Alcohol, Inflammation, and Microbiota in Alcoholic Liver Disease. Int J Mol Sci. 2023; 24(4). PMC: 9966185. DOI: 10.3390/ijms24043735. View

18.

Peng L, Li Z, Green R, Holzman I, Lin J . Butyrate enhances the intestinal barrier by facilitating tight junction assembly via activation of AMP-activated protein kinase in Caco-2 cell monolayers. J Nutr. 2009; 139(9):1619-25. PMC: 2728689. DOI: 10.3945/jn.109.104638. View

19.

Zhou Y, Zhang M, Zhao X, Feng J . Ammonia exposure induced intestinal inflammation injury mediated by intestinal microbiota in broiler chickens via TLR4/TNF-α signaling pathway. Ecotoxicol Environ Saf. 2021; 226:112832. DOI: 10.1016/j.ecoenv.2021.112832. View

20.

Zhang L, Chen C, Chai D, Li C, Qiu Z, Kuang T . Characterization of the intestinal fungal microbiome in patients with hepatocellular carcinoma. J Transl Med. 2023; 21(1):126. PMC: 9933289. DOI: 10.1186/s12967-023-03940-y. View