Circulating MicroRNAs As Biomarkers for the Early Diagnosis of Lung Cancer and Its Differentiation from Tuberculosis

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2024 Dec 17

PMID 39682592

Authors

Yeldar Ashirbekov

Nazgul Khamitova

Kantemir Satken

Arman Abaildayev

Ilya Pinskiy

Askar Yeleussizov

Laura Yegenova

Anargul Kairanbayeva

Danara Kadirshe

Gulzhakhan Utegenova

Nurlan Jainakbayev

Kamalidin Sharipov

Affiliations

Soon will be listed here.

Abstract

Background: The differential diagnosis of tuberculosis (TB) and lung cancer (LC) is often challenging due to similar clinicopathological presentations when bacterial shedding is negative, which can lead to delays in treatment. In this study, we tested the potential of plasma-circulating microRNAs (miRNAs) for the early and differential diagnosis of TB and LC.

Methods: We conducted a two-phase study: profiling 188 miRNAs in pooled plasma samples and validating 14 selected miRNAs in individual plasma samples from 68 LC patients, 38 pulmonary TB patients, and 41 healthy controls.

Results: Twelve miRNAs were significantly elevated in LC patients compared to controls and TB patients, while two miRNAs were significantly elevated in TB patients compared to controls. ROC analysis demonstrated that miR-130b-3p, miR-1-3p, miR-423-5p, and miR-200a-3p had good discriminatory ability to distinguish LC patients (including those with stage I tumours) from healthy individuals and miR-130b-3p, miR-423-5p, miR-15b-5p, and miR-18b-5p effectively distinguished LC patients (including those with stage I tumours) from TB patients. Additionally, miR-18b-5p showed good discriminatory ability between SCLC and NSCLC patients.

Conclusions: Circulating miRNAs hold strong potential for the early detection of LC and for distinguishing LC from TB.

Citing Articles

miRNAs and Hematological Markers in Non-Alcoholic Fatty Liver Disease-A New Diagnostic Path?.

Michalak A, Guz M, Kozicka J, Cybulski M, Jeleniewicz W, Telejko I Biomedicines. 2025; 13(1).

PMID: 39857813 PMC: 11762403. DOI: 10.3390/biomedicines13010230.

References

Moreno-Moya J, Vilella F, Simon C . MicroRNA: key gene expression regulators. Fertil Steril. 2013; 101(6):1516-23. DOI: 10.1016/j.fertnstert.2013.10.042. View

Yan W, Wang Y, Chen Y, Guo Y, Li Q, Wei X . Exosomal miR-130b-3p Promotes Progression and Tubular Formation Through Targeting PTEN in Oral Squamous Cell Carcinoma. Front Cell Dev Biol. 2021; 9:616306. PMC: 8019696. DOI: 10.3389/fcell.2021.616306. View

Bray F, Laversanne M, Sung H, Ferlay J, Siegel R, Soerjomataram I . Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024; 74(3):229-263. DOI: 10.3322/caac.21834. View

Wagh V, Urhekar A, Modi D . Levels of microRNA miR-16 and miR-155 are altered in serum of patients with tuberculosis and associate with responses to therapy. Tuberculosis (Edinb). 2017; 102:24-30. DOI: 10.1016/j.tube.2016.10.007. View

Li W, Zhang B, Jia Y, Shi H, Wang H, Guo Q . LncRNA LOXL1-AS1 regulates the tumorigenesis and development of lung adenocarcinoma through sponging miR-423-5p and targeting MYBL2. Cancer Med. 2019; 9(2):689-699. PMC: 6970024. DOI: 10.1002/cam4.2641. View

Zhang X, Guo J, Fan S, Li Y, Wei L, Yang X . Screening and identification of six serum microRNAs as novel potential combination biomarkers for pulmonary tuberculosis diagnosis. PLoS One. 2013; 8(12):e81076. PMC: 3857778. DOI: 10.1371/journal.pone.0081076. View

Qian X, Xie F, Wei H, Cui D . Identification of Key Circulating Exosomal microRNAs in Gastric Cancer. Front Oncol. 2021; 11:693360. PMC: 8323470. DOI: 10.3389/fonc.2021.693360. View

Wang J, Ye J, Dang Y, Xu S . LncRNA PGM5-AS1 inhibits non-small cell lung cancer progression by targeting miRNA-423-5p/SLIT2 axis. Cancer Cell Int. 2024; 24(1):216. PMC: 11191156. DOI: 10.1186/s12935-024-03402-5. View

Chen Y, Chen X, Li Z, Zhu Y, Liu F, Cai J . CircDENND2D Inhibits PD-L1-Mediated Non-Small Cell Lung Cancer Metastasis and Immune Escape by Regulating miR-130b-3p/STK11 Axis. Biochem Genet. 2023; 61(6):2691-2709. DOI: 10.1007/s10528-023-10401-0. View

10.

Varol Y, Varol U, Unlu M, Kayaalp I, Ayranci A, Dereli M . Primary lung cancer coexisting with active pulmonary tuberculosis. Int J Tuberc Lung Dis. 2014; 18(9):1121-5. DOI: 10.5588/ijtld.14.0152. View

11.

Schwarzenbach H . Clinical Relevance of Circulating, Cell-Free and Exosomal microRNAs in Plasma and Serum of Breast Cancer Patients. Oncol Res Treat. 2017; 40(7-8):423-429. DOI: 10.1159/000478019. View

12.

Zhu W, Zhou K, Zha Y, Chen D, He J, Ma H . Diagnostic Value of Serum miR-182, miR-183, miR-210, and miR-126 Levels in Patients with Early-Stage Non-Small Cell Lung Cancer. PLoS One. 2016; 11(4):e0153046. PMC: 4836744. DOI: 10.1371/journal.pone.0153046. View

13.

Li X, Xu Y, Liao P . Diagnostic performance of microRNA-29a in active pulmonary tuberculosis: a systematic review and meta-analysis. Clinics (Sao Paulo). 2023; 78:100290. PMC: 10589768. DOI: 10.1016/j.clinsp.2023.100290. View

14.

Uno S, Nishimura T, Nishio K, Kohsaka A, Tamizu E, Nakano Y . Potential biomarker enhancing the activity of tuberculosis, hsa-miR-346. Tuberculosis (Edinb). 2021; 129:102101. DOI: 10.1016/j.tube.2021.102101. View

15.

Kang H, Yun S, Shin S, Youn D, Son G, Lee J . Tuberculous Pleural Effusion-Derived Exosomal miR-130b-3p and miR-423-5p Promote the Proliferation of Lung Cancer Cells via Cyclin D1. Int J Mol Sci. 2024; 25(18). PMC: 11431986. DOI: 10.3390/ijms251810119. View

16.

Arab A, Karimipoor M, Irani S, Kiani A, Zeinali S, Tafsiri E . Potential circulating miRNA signature for early detection of NSCLC. Cancer Genet. 2017; 216-217:150-158. DOI: 10.1016/j.cancergen.2017.07.006. View

17.

Nassar F, Talhouk R, Zgheib N, Tfayli A, El Sabban M, El Saghir N . microRNA Expression in Ethnic Specific Early Stage Breast Cancer: an Integration and Comparative Analysis. Sci Rep. 2017; 7(1):16829. PMC: 5715135. DOI: 10.1038/s41598-017-16978-y. View

18.

Li H, Liu P, Li D, Wang Z, Ding Z, Zhou M . STAT3/miR-130b-3p/MBNL1 feedback loop regulated by mTORC1 signaling promotes angiogenesis and tumor growth. J Exp Clin Cancer Res. 2022; 41(1):297. PMC: 9552455. DOI: 10.1186/s13046-022-02513-z. View

19.

Wang C, Liu C, Wei L, Shi L, Pan Z, Mao L . A Group of Novel Serum Diagnostic Biomarkers for Multidrug-Resistant Tuberculosis by iTRAQ-2D LC-MS/MS and Solexa Sequencing. Int J Biol Sci. 2016; 12(2):246-56. PMC: 4737680. DOI: 10.7150/ijbs.13805. View

20.

Yu H, Guan Z, Cuk K, Zhang Y, Brenner H . Circulating MicroRNA Biomarkers for Lung Cancer Detection in East Asian Populations. Cancers (Basel). 2019; 11(3). PMC: 6468694. DOI: 10.3390/cancers11030415. View